FastQCFastQC Report
Mon 6 Jun 2016
ERR1211583_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211583_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10834136
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285610.2636204677511894No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235680.21753465158643018No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156680.1446169773021125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA153850.045.8034671
GTATCAA260600.037.3758351
TCAACGC308100.030.6379724
CAACGCA311600.030.3049955
ATCAACG312100.030.2146383
AACGCAG324050.029.266976
TATCAAC334800.028.5735242
GTACATG337850.027.5313971
TACATGG342900.026.4027672
ACGCAGA369500.025.4131137
ACATGGG348050.025.1363243
GTACCTG91950.024.776541
TACCTGG88850.024.679732
CGCAGAG381100.024.542458
CATGGGG211350.023.1874944
GCAGAGT433350.020.8461599
GAGTACT262900.019.9261312-13
AGAGTAC392850.019.0148110-11
ATGGGGG131400.018.9851825
ACCTGGG116550.018.5039393
Produced by FastQC (version 0.11.5)