FastQCFastQC Report
Mon 6 Jun 2016
ERR1211583_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211583_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10834136
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340830.3145889990673922No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285560.26357431732442715No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201170.18568162703514152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA176400.043.9367031
GTATCAA292750.036.8131981
TCAACGC358300.029.520764
CAACGCA362950.029.1683065
ATCAACG362950.029.1169243
AACGCAG376450.028.1732626
TATCAAC383800.027.7918782
GTACATG363050.026.334541
TACATGG368900.025.370222
ACATGGG369050.024.4593543
ACGCAGA433700.024.334997
CGCAGAG443700.023.6488558
TACCTGG86150.023.4197272
CATGGGG221400.023.091394
GTACCTG90850.022.9362071
GCAGAGT498150.020.7620759
GAGTACT310400.019.80896812-13
ATGGGGG125000.019.5850435
AGAGTAC461550.019.19639210-11
AGTACTT335650.018.29779612-13
Produced by FastQC (version 0.11.5)