FastQCFastQC Report
Mon 6 Jun 2016
ERR1211582_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211582_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5433488
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190360.350345855185472No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167250.3078133235961872No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110940.20417823688945297No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69090.12715588955013796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89500.050.594941
GTACCTG58450.041.3184781
TACCTGG57350.040.937732
GTATCAA153050.037.1683121
ACCTGGG69700.032.5634843
TCAACGC181500.030.5757984
ATCAACG183050.030.1893753
CAACGCA184750.029.9851285
AACGCAG192700.029.1317676
TATCAAC191550.029.0332
GTACATG169550.027.9871731
TACATGG173450.026.8545252
TATAACG6050.026.441142
ACGCAGA216450.025.7401897
ACATGGG176150.025.6897683
CGCAGAG221850.024.9879828
TAACGCA6800.024.1859784
CATGGGG109400.024.1392354
GCAGAGT244400.021.8941029
CCTGGGG67200.021.7468154
Produced by FastQC (version 0.11.5)