FastQCFastQC Report
Mon 6 Jun 2016
ERR1211582_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211582_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5433488
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227770.41919665599703176No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201160.37022258998271457No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141180.2598330943217322No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75320.1386218208266955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103750.052.2074741
TACCTGG59700.042.6181372
GTACCTG61700.042.556981
TATAACG4900.036.4719542
GTATCAA174350.036.447581
ACCTGGG70850.035.1495443
TCAACGC217500.028.7756184
ATCAACG218850.028.5554353
CAACGCA221100.028.3078695
TATCAAC227200.027.6961442
AACGCAG229450.027.1967936
GTACATG183800.026.5970821
TAACGCA6600.026.3413164
TACATGG186600.025.5310862
ACATGGG186350.024.6852173
ACGCAGA256700.024.291417
ATAACGC7000.024.1650753
CCTGGGG68950.023.9195254
CGCAGAG263750.023.5886558
CATGGGG120650.023.094424
Produced by FastQC (version 0.11.5)