FastQCFastQC Report
Mon 6 Jun 2016
ERR1211581_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211581_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4424891
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215470.48694984803015484No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174710.3948345846259264No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122670.27722716785566015No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96540.2181748657763547No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA63410.14330296497699038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG75550.065.94622
GTACCTG80650.062.6719741
ACCTGGG79150.060.7306183
TATCACG4000.043.518112
TAACGCA6500.041.9273644
TATAACG6050.041.214382
CCTGGGG48950.038.012414
ATCACGC4900.035.4804043
GGTATCA157500.030.7748181
TCACGCA6350.028.8585054
CCTGGGA72200.028.1795234
GTATCAA236900.027.6452241
CATGGGG81200.025.6348424
GTACATG267400.025.1620221
GTACACG6200.025.096041
ACATGGG263200.024.1721733
TACATGG275700.024.0778922
TCAACGC266500.023.6789114
ATCAACG267900.023.5551683
CAACGCA268750.023.4949655
Produced by FastQC (version 0.11.5)