FastQCFastQC Report
Mon 6 Jun 2016
ERR1211581_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211581_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4424891
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253710.5733700558951621No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207730.4694578917311183No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159920.36141003247311626No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101700.22983616997571238No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA83490.1886826138768164No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45330.10244320142575264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG72650.065.9525762
GTACCTG77250.062.488631
ACCTGGG78350.059.1332323
TATAACG7150.054.583982
TAACGCA7350.053.7014054
CCTGGGG48050.038.040824
GTACCCG4000.036.5343361
TATCACG4550.029.9695172
GTACACG5800.028.4472031
GTATAAC19100.027.6429351
GGTATCA193650.026.5343531
ATAACGC15250.026.4985623
CCTGGGA70600.026.4893974
GTATCAA276000.025.7226331
TACCCGG5500.025.6478942
ATCACGC5700.025.5552753
CATGGGG77800.025.064684
GGGTATG16200.023.7852237
GGATGAC31150.022.9294668
GTACATG291450.022.9195081
Produced by FastQC (version 0.11.5)