##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1211581_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4424891
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.96509066551018	33.0	31.0	34.0	30.0	34.0
2	32.21423058782691	33.0	31.0	34.0	30.0	34.0
3	32.30057350565246	34.0	31.0	34.0	30.0	34.0
4	35.97160472427456	37.0	35.0	37.0	35.0	37.0
5	35.87992382185234	37.0	35.0	37.0	35.0	37.0
6	35.91498186057012	37.0	35.0	37.0	35.0	37.0
7	35.8313816091741	37.0	35.0	37.0	35.0	37.0
8	35.80975599172952	37.0	35.0	37.0	35.0	37.0
9	37.434800088860946	39.0	37.0	39.0	34.0	39.0
10-11	37.37981647457531	39.0	37.0	39.0	34.0	39.0
12-13	37.29755252728259	39.0	37.0	39.0	34.0	39.0
14-15	38.48080336894175	40.0	38.0	41.0	34.0	41.0
16-17	38.42442005916078	40.0	38.0	41.0	34.0	41.0
18-19	38.463393335564646	40.0	38.0	41.0	34.0	41.0
20-21	38.40789976521456	40.0	38.0	41.0	34.0	41.0
22-23	38.27914778013741	40.0	38.0	41.0	34.0	41.0
24-25	38.17136343019523	40.0	37.0	41.0	33.0	41.0
26-27	37.953214327765366	40.0	37.0	41.0	33.0	41.0
28-29	37.95260052733502	40.0	37.0	41.0	33.0	41.0
30-31	37.7866203935871	40.0	37.0	41.0	33.0	41.0
32-33	37.52619465654634	40.0	36.0	41.0	32.5	41.0
34-35	37.33346075643445	40.0	36.0	41.0	31.5	41.0
36-37	37.190211804087376	40.0	36.0	41.0	31.0	41.0
38-39	37.09451724347561	40.0	36.0	41.0	31.0	41.0
40-41	36.94049943829125	39.0	36.0	41.0	30.5	41.0
42-43	36.745034058466075	39.0	35.0	41.0	30.0	41.0
44-45	36.52129815175108	39.0	35.0	41.0	30.0	41.0
46-47	36.22447920186056	39.0	35.0	41.0	28.5	41.0
48-49	35.93568157950106	39.0	35.0	40.0	27.0	41.0
50-51	35.725876637413215	39.0	35.0	40.0	26.5	41.0
52-53	35.54681132258399	39.0	35.0	40.0	26.0	41.0
54-55	35.35064027565877	38.0	34.0	40.0	25.0	41.0
56-57	35.15678239305782	38.0	34.0	40.0	25.0	41.0
58-59	34.92181841315413	38.0	34.0	40.0	24.0	41.0
60-61	34.6234928724798	38.0	33.5	40.0	23.5	41.0
62-63	34.29433278695453	37.5	33.0	40.0	23.0	41.0
64-65	34.010216070859144	37.0	32.5	40.0	22.0	41.0
66-67	33.66889771974044	36.5	32.0	39.0	22.0	41.0
68-69	33.29327818470557	36.0	31.5	39.0	21.5	40.0
70-71	32.92819145149564	35.5	31.0	39.0	20.0	40.0
72-73	32.50756120320251	35.0	31.0	38.0	20.0	40.0
74-75	32.099754999614674	35.0	30.5	37.5	19.0	39.5
76-77	30.608797821234468	33.5	28.5	36.0	18.5	38.5
78-79	31.458294678897175	34.0	30.0	36.0	19.0	39.0
80-81	31.397783357827347	34.0	30.0	36.0	19.0	38.5
82-83	31.09390547699367	34.0	30.0	36.0	18.5	37.0
84-85	30.7382961523798	34.0	29.5	35.0	18.0	37.0
86-87	30.43405430777843	34.0	29.0	35.0	18.0	36.5
88-89	30.12505223292506	34.0	29.0	35.0	17.0	36.0
90-91	29.816421127661677	34.0	29.0	35.0	15.0	36.0
92-93	29.55651822835862	34.0	29.0	35.0	13.0	35.0
94-95	29.334732878165813	34.0	27.5	35.0	11.5	35.0
96-97	29.08996956083212	33.0	27.0	35.0	11.0	35.0
98-99	28.875844060339567	33.0	27.0	35.0	10.0	35.0
100	27.159729358305096	31.0	24.0	34.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.14966281798763603
1101	2	0.06929684476575915
1101	3	0.09116008914915597
1101	4	0.053199538545911196
1101	5	0.01554913060934382
1101	6	0.07560846835934854
1101	7	0.0673118534696755
1101	8	0.10334088872495073
1101	9	0.06780836804323798
1101	10-11	0.12804194979345596
1101	12-13	0.06913781399342156
1101	14-15	0.06495402820694096
1101	16-17	0.10950397427185266
1101	18-19	0.0972829691528716
1101	20-21	-0.00579092723762642
1101	22-23	-0.02659655416718465
1101	24-25	-0.06236977141382738
1101	26-27	-0.05142793409746815
1101	28-29	0.018270258148447738
1101	30-31	0.0627214328210286
1101	32-33	-0.028607611485689688
1101	34-35	-0.11461948621299456
1101	36-37	-0.12771386529562534
1101	38-39	-0.24350183571991124
1101	40-41	-0.10842378394322338
1101	42-43	-0.06969727472161935
1101	44-45	-0.21285081638171732
1101	46-47	-0.24199024088613896
1101	48-49	-0.28785740927418857
1101	50-51	-0.3511941198040347
1101	52-53	-0.3141965671343243
1101	54-55	-0.35805954098692894
1101	56-57	-0.4644677898945133
1101	58-59	-0.5886412335925044
1101	60-61	-0.5357288645199745
1101	62-63	-0.6120694845491457
1101	64-65	-0.48361204930509416
1101	66-67	-0.6299887925161514
1101	68-69	-0.5850570912764113
1101	70-71	-0.6116871719782822
1101	72-73	-0.7571113688645568
1101	74-75	-0.7198537673202665
1101	76-77	-0.6413488749410199
1101	78-79	-0.3747068853969999
1101	80-81	-0.4227125877405342
1101	82-83	-0.5873122045580246
1101	84-85	-0.6197746752483404
1101	86-87	-0.6135775823685741
1101	88-89	-0.7128575900304703
1101	90-91	-0.8186736456020611
1101	92-93	-0.9338604424027928
1101	94-95	-0.8971915500329253
1101	96-97	-0.8848561964429678
1101	98-99	-0.9925088975815086
1101	100	-0.8117928753585133
1102	1	0.2386214457889686
1102	2	0.22251381031383488
1102	3	0.17150156247159032
1102	4	0.09658884768494858
1102	5	0.06426883377908865
1102	6	0.10233730039418276
1102	7	0.08229565857602239
1102	8	0.06489260025053056
1102	9	0.06983214689320505
1102	10-11	0.13763898044800982
1102	12-13	0.2066820503124731
1102	14-15	0.09813604942573306
1102	16-17	0.1914966744487998
1102	18-19	0.09781568767137827
1102	20-21	0.10247993764204466
1102	22-23	0.07194245840181424
1102	24-25	0.1637145138310956
1102	26-27	0.03684269713812682
1102	28-29	0.14554756714228745
1102	30-31	0.06417692009000575
1102	32-33	0.12805680748486026
1102	34-35	0.09652361140705779
1102	36-37	0.0868174223785445
1102	38-39	0.09112102739569394
1102	40-41	0.08313041393635956
1102	42-43	0.14121982490105012
1102	44-45	0.07665507503385527
1102	46-47	0.022110517469386082
1102	48-49	0.04771805482892688
1102	50-51	0.1148663708475226
1102	52-53	-3.684528631211492E-4
1102	54-55	0.047926244729261214
1102	56-57	0.12051891370195733
1102	58-59	0.09763173077471521
1102	60-61	-0.037306613822593704
1102	62-63	0.02364440614764618
1102	64-65	0.06626659866984141
1102	66-67	-0.1391631830999529
1102	68-69	-0.08013682783219167
1102	70-71	-0.18826023210447573
1102	72-73	-0.2723561935104186
1102	74-75	-0.3679805008803356
1102	76-77	-0.16556884091021118
1102	78-79	-0.10015601975322497
1102	80-81	-0.030811679100732903
1102	82-83	-0.023118319484733973
1102	84-85	-0.11027879843953414
1102	86-87	-0.03152539320130998
1102	88-89	-0.2571643783384552
1102	90-91	-0.42751668777426843
1102	92-93	-0.431144304962519
1102	94-95	-0.2692403082422459
1102	96-97	-0.34983299974780024
1102	98-99	-0.38103354906201403
1102	100	-0.45804852599784596
1103	1	0.2776923659457715
1103	2	0.20596501596267558
1103	3	0.16496698445959623
1103	4	0.11149876595643349
1103	5	0.11883515714894344
1103	6	0.08252746481925044
1103	7	0.09915097062865641
1103	8	0.12826618887275743
1103	9	0.1754217741896582
1103	10-11	0.14747110780882622
1103	12-13	0.11143807291107777
1103	14-15	0.14950222862989193
1103	16-17	0.15519525313102633
1103	18-19	0.12904872738905482
1103	20-21	0.0667947885300677
1103	22-23	0.06774304545246679
1103	24-25	0.035855908820849436
1103	26-27	-0.05117929565750501
1103	28-29	0.07897857664688956
1103	30-31	0.1754494698791902
1103	32-33	0.22535532667014735
1103	34-35	0.13886739088172817
1103	36-37	0.1447327075702347
1103	38-39	0.15891827165703631
1103	40-41	0.19305434605865202
1103	42-43	0.1242501709062438
1103	44-45	0.11339090540249686
1103	46-47	0.22283211373407852
1103	48-49	0.1600294677806815
1103	50-51	0.1474729440865019
1103	52-53	0.05887045886655784
1103	54-55	0.07986596130878354
1103	56-57	-0.01441707362904765
1103	58-59	-0.07707146569605783
1103	60-61	0.009560409434364203
1103	62-63	-0.035101382433119
1103	64-65	-0.18820971147127352
1103	66-67	-0.21357012930896246
1103	68-69	-0.14493415805698362
1103	70-71	-0.01997614258625191
1103	72-73	-0.1407992777951037
1103	74-75	-0.21381948544560458
1103	76-77	-0.04235105842727549
1103	78-79	0.011618234476113543
1103	80-81	-0.15124791882708166
1103	82-83	-0.20776060835900267
1103	84-85	-0.20651286113745826
1103	86-87	-0.0813605646622797
1103	88-89	-0.19463190680764697
1103	90-91	-0.16083228643901748
1103	92-93	-0.27300300060323934
1103	94-95	-0.25815696839766744
1103	96-97	-0.3130093240555709
1103	98-99	-0.2963660131255992
1103	100	-0.30221724502333913
1104	1	0.2260764918667384
1104	2	0.10881286386459266
1104	3	0.15982275703748172
1104	4	0.10955720834108007
1104	5	0.0534492283101784
1104	6	0.12644927916432636
1104	7	0.16523640036407983
1104	8	0.16097798370023497
1104	9	0.17231153092779294
1104	10-11	0.1981831222993904
1104	12-13	0.1579479805565569
1104	14-15	0.1977741673306852
1104	16-17	0.16622852188582016
1104	18-19	0.20017823078242714
1104	20-21	0.11162713938122693
1104	22-23	0.21208480040449018
1104	24-25	0.07274467827544129
1104	26-27	0.07428103654990537
1104	28-29	0.19212434711216986
1104	30-31	0.14620982225239487
1104	32-33	0.09930457489424072
1104	34-35	0.14168017371295605
1104	36-37	0.15787505705299765
1104	38-39	0.14826303647348738
1104	40-41	0.17042312137091642
1104	42-43	0.04931715014268434
1104	44-45	0.08718820498694413
1104	46-47	0.2228869544843448
1104	48-49	0.06715609454789018
1104	50-51	0.009864672991042767
1104	52-53	-0.028669182518449077
1104	54-55	-0.10898930292310638
1104	56-57	0.0996432094842632
1104	58-59	-0.015439014856170274
1104	60-61	-0.04980466521507054
1104	62-63	0.055384730178417385
1104	64-65	-0.02068449823003249
1104	66-67	0.01140035721477517
1104	68-69	-0.09237106193577915
1104	70-71	0.09128103547528355
1104	72-73	0.0848619321043742
1104	74-75	-0.1010688534640991
1104	76-77	0.1019482343296012
1104	78-79	0.15893189295882948
1104	80-81	0.19791436057956346
1104	82-83	0.04632468993866823
1104	84-85	-0.06099926963397806
1104	86-87	-0.15016470021001282
1104	88-89	-0.08768824258728003
1104	90-91	-0.039246141898306774
1104	92-93	-0.05196476842295894
1104	94-95	-0.15464103862263912
1104	96-97	-0.1578601902243335
1104	98-99	-0.17401900006344562
1104	100	-0.03880924424551324
1105	1	0.25652800966538436
1105	2	0.1624871197201756
1105	3	0.08330989952449386
1105	4	0.1045352560123547
1105	5	0.1225954925005226
1105	6	0.07129214526412397
1105	7	0.09186355949243108
1105	8	0.10282329433997006
1105	9	0.047495918356453615
1105	10-11	0.11459867178077587
1105	12-13	0.13015361818384008
1105	14-15	0.1822081551635648
1105	16-17	0.15588315919295326
1105	18-19	0.16668852771223186
1105	20-21	0.12127303822126834
1105	22-23	0.1396778287300009
1105	24-25	0.14359927804953543
1105	26-27	0.15064071893974784
1105	28-29	0.1041183897691198
1105	30-31	0.23365109615279067
1105	32-33	0.19338408975912813
1105	34-35	0.21660450588703384
1105	36-37	0.21812216947444796
1105	38-39	0.12774693855569552
1105	40-41	0.13995073829280358
1105	42-43	0.13295076738079104
1105	44-45	0.17726053436815903
1105	46-47	0.14301874966744066
1105	48-49	0.05161676939120241
1105	50-51	0.13117873453584394
1105	52-53	0.07317774450776682
1105	54-55	0.012165443773866969
1105	56-57	-0.05742515955438421
1105	58-59	0.028697973702314528
1105	60-61	0.04836901864321419
1105	62-63	0.031244971650117748
1105	64-65	0.11508423639374854
1105	66-67	0.021279908047191043
1105	68-69	-0.01166875962219649
1105	70-71	0.030905355751507102
1105	72-73	0.059533195101018066
1105	74-75	0.06860197314733796
1105	76-77	0.009258400891770435
1105	78-79	0.14092860578448452
1105	80-81	0.08506944992699061
1105	82-83	0.09257895398968685
1105	84-85	0.05035155981102335
1105	86-87	0.08674988315756949
1105	88-89	-0.03098120735318588
1105	90-91	-0.01944122467842746
1105	92-93	0.03075376076710512
1105	94-95	0.03954979360896971
1105	96-97	0.004912007497694049
1105	98-99	-0.02801348536763726
1105	100	0.1573396953971269
1106	1	0.30833782315642466
1106	2	0.19238168938788647
1106	3	0.18505044036331952
1106	4	0.1364999477847917
1106	5	0.16291493523213063
1106	6	0.13499233347399553
1106	7	0.14597278606075292
1106	8	0.09733063985201085
1106	9	0.10393506637336714
1106	10-11	0.17194666322293983
1106	12-13	0.1563460352468553
1106	14-15	0.20070634185213265
1106	16-17	0.17263600882068886
1106	18-19	0.15139826279484936
1106	20-21	0.16930435645117115
1106	22-23	0.19838956349767756
1106	24-25	0.1715892263541008
1106	26-27	0.16394612260830144
1106	28-29	0.19668853499422312
1106	30-31	0.36279009109706095
1106	32-33	0.3295049350437651
1106	34-35	0.35358235905693647
1106	36-37	0.3142497438155232
1106	38-39	0.3291425961241856
1106	40-41	0.3253989815820546
1106	42-43	0.3978957210499132
1106	44-45	0.3069718003684869
1106	46-47	0.26179214290556985
1106	48-49	0.23463180270199047
1106	50-51	0.26351458120745974
1106	52-53	0.21255810800975183
1106	54-55	0.17120807004158678
1106	56-57	0.311244861471053
1106	58-59	0.2288011139940167
1106	60-61	0.11144349832843403
1106	62-63	0.3630689614918836
1106	64-65	0.36743929855742863
1106	66-67	0.43319862972921896
1106	68-69	0.3271340679778234
1106	70-71	0.24691853995851432
1106	72-73	0.3369987747961787
1106	74-75	0.3769102968687008
1106	76-77	0.3263245160231918
1106	78-79	0.3742849144398761
1106	80-81	0.35565498542995755
1106	82-83	0.40902158416897905
1106	84-85	0.27129320882101027
1106	86-87	0.24184162752056082
1106	88-89	0.3185000762152903
1106	90-91	0.3275053774947523
1106	92-93	0.2999713688027228
1106	94-95	0.24766202692551786
1106	96-97	0.30271880467675416
1106	98-99	0.2260145162484477
1106	100	0.12357515317308909
1107	1	0.20996117477215748
1107	2	0.13578144699677352
1107	3	0.08079141331719342
1107	4	0.07201423416216102
1107	5	-0.04324724597846341
1107	6	0.04931519972610232
1107	7	0.06056684436963877
1107	8	0.030190184709667278
1107	9	0.08426475307686587
1107	10-11	0.04170682210189369
1107	12-13	0.12146107396596051
1107	14-15	0.13900697765912184
1107	16-17	0.11569271616568244
1107	18-19	0.0816677889712949
1107	20-21	0.060412433068599114
1107	22-23	0.1346124136003013
1107	24-25	0.04189241801650212
1107	26-27	0.13699590744450063
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2316	68-69	-0.1902654678518232
2316	70-71	-0.3502504957267192
2316	72-73	-0.5072048184469118
2316	74-75	-0.24440365552865018
2316	76-77	-0.19503836251324103
2316	78-79	-0.425416656279868
2316	80-81	-0.20352345788000648
2316	82-83	-0.08717667056037826
2316	84-85	-0.04048336419401011
2316	86-87	0.0629868640356328
2316	88-89	0.07424448577134868
2316	90-91	0.1300595749738349
2316	92-93	0.15839088399757983
2316	94-95	0.2576989672147718
2316	96-97	0.3412360383064943
2316	98-99	0.30642087180616784
2316	100	0.08121324816322684
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	10.0
11	20.0
12	96.0
13	211.0
14	713.0
15	2042.0
16	5266.0
17	11995.0
18	21784.0
19	29949.0
20	36549.0
21	45483.0
22	54068.0
23	55307.0
24	53186.0
25	54803.0
26	61862.0
27	73295.0
28	86411.0
29	102288.0
30	121374.0
31	144642.0
32	175131.0
33	217654.0
34	280545.0
35	377163.0
36	536081.0
37	836346.0
38	895280.0
39	145290.0
40	44.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.00587733498884	20.58997658355547	13.173092500053812	22.231053581401884
2	14.29916836108102	22.761982091604676	41.94043049810128	20.99841904921303
3	16.04816177409473	31.52183910955364	31.117101036716154	21.312898079635474
4	10.284702205777663	18.727346607804947	40.90221237678069	30.0857388096367
5	10.4893941732644	39.06185909264411	36.436951157308485	14.011795576783005
6	26.11544671203454	40.03637173933481	21.27848449127476	12.569697057355885
7	25.12466408777075	33.51366169245751	25.258294498101762	16.10337972166998
8	25.633568354057918	35.4947887593229	22.421374878018685	16.450268008600503
9	27.170251199408074	15.191605849726017	22.34479900182852	35.293343949037386
10-11	22.743331732534816	27.599887002457695	31.03583717054154	18.620944094465948
12-13	24.172768609796556	25.77423488413088	30.63636486907942	19.41663163699314
14-15	21.594767687957823	26.683449008551403	28.999959095019108	22.72182420847167
16-17	19.983818797653047	29.244381919314332	29.913607973084062	20.858191309948555
18-19	19.838929787412678	27.989619060845243	32.40302108064686	19.76843007109522
20-21	20.698682209260017	27.58188450177747	32.45777803893031	19.261655250032206
22-23	20.249429929331576	27.786476047992153	31.858204384741768	20.105889637934503
24-25	19.967942705920372	27.989023455950317	32.23266567078504	19.810368167344272
26-27	19.996282387159432	27.842027443666584	32.298123170502265	19.86356699867172
28-29	20.047328489594715	27.422455020194047	32.67898641729752	19.851230072913715
30-31	20.458413628810142	27.795791134656167	32.31751786463713	19.428277371896556
32-33	20.02790674969083	27.951916227318417	32.48757665994084	19.53260036304991
34-35	20.194483417617842	28.141323155308296	32.246045830041204	19.418147597032657
36-37	20.548545029401797	27.83984503757163	31.79062082715558	19.820989105870993
38-39	20.342397403328533	28.436565164315248	31.680912884967405	19.540124547388814
40-41	20.368716941119807	28.164213763252228	32.0200317023975	19.447037593230462
42-43	20.30533855961912	27.995150421467613	31.605403519018367	20.094107499894896
44-45	20.22856082530241	28.452463057221117	31.377893356136603	19.941082761339867
46-47	20.25865629585516	28.369326249449813	31.096116652418665	20.275900802276364
48-49	20.123132187593686	28.533134505320206	31.129688833632095	20.214044473454006
50-51	20.220130281417582	28.70841269455624	30.7627435148862	20.308713509139984
52-53	20.268086444606386	28.859300890596817	30.355109843437887	20.51750282135891
54-55	20.35004076614359	28.929825166073304	30.35064533681084	20.369488730972268
56-57	20.46929060611957	28.913484036620613	30.18895428660926	20.428271070650556
58-59	20.373698415159293	28.781777156832923	30.559361127897816	20.28516330010997
60-61	20.711114817741695	29.33348340666925	30.10959308767639	19.845808687912665
62-63	20.201784706474527	29.02130224671586	30.678600722498505	20.098312324311106
64-65	20.17373052444727	28.7627440825385	30.39160900642291	20.671916386591324
66-67	20.29010401475506	28.902898203679428	30.093706284065163	20.71329149750035
68-69	20.320630311808426	28.992977976019745	30.253538304918177	20.432853407253656
70-71	20.38492861817115	28.807077014765213	30.276467924705695	20.531526442357944
72-73	20.398542457540987	29.02812910481926	29.849513928372566	20.72381450926718
74-75	20.389780852801	29.04600763356622	29.825546737935888	20.738664775696893
76-77	20.128965083624013	28.86618395068034	30.459991963271516	20.544859002424126
78-79	20.24257144994746	28.80026834345159	30.10417069283558	20.852989513765372
80-81	19.948178331269492	29.338852781394554	29.71953151891573	20.99343736842022
82-83	20.382462322853588	29.229281087057423	29.617498121977064	20.77075846811192
84-85	19.933925845720353	29.39654495240819	29.578322342637325	21.091206859234134
86-87	19.933271340761895	29.164268862113847	30.06860471056145	20.833855086562817
88-89	20.191134825874638	28.964845147247907	29.970811371980794	20.873208654896658
90-91	20.47030988294587	29.257496706799962	29.789992514992058	20.48220089526211
92-93	20.228228195644576	29.292864218193426	29.76683263190303	20.71207495425897
94-95	19.87058696802381	29.009219551050013	29.845508912051834	21.274684568874342
96-97	20.359605602896337	29.009662823974942	29.802982277175154	20.827749295953566
98-99	20.232001464701373	28.802685737922427	29.825806772435588	21.13950602494061
100	19.232052433688228	29.328760726089765	30.054660619083162	21.384526221138845
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2190.0
1	1664.0
2	3564.5
3	5101.5
4	3115.0
5	2238.0
6	3081.5
7	5495.0
8	8291.5
9	9401.0
10	8452.0
11	7342.5
12	7693.0
13	8402.5
14	9065.5
15	9823.5
16	10481.0
17	11169.0
18	11876.5
19	12684.0
20	14055.5
21	15805.5
22	18058.0
23	21214.0
24	24859.0
25	29698.5
26	36812.5
27	44181.0
28	50931.5
29	60321.5
30	73648.5
31	86136.0
32	100614.5
33	119864.0
34	140098.5
35	155012.5
36	170709.5
37	185354.5
38	190350.0
39	193260.0
40	185977.5
41	173819.0
42	165198.5
43	158714.0
44	156513.5
45	156791.0
46	151197.5
47	140872.5
48	136015.0
49	134157.5
50	138261.5
51	129530.0
52	107775.5
53	95466.0
54	87048.5
55	77907.0
56	68098.0
57	59237.5
58	51293.5
59	42377.0
60	33251.5
61	25612.0
62	19750.5
63	14786.0
64	10826.5
65	8037.0
66	5930.5
67	4494.5
68	3508.5
69	2760.0
70	2169.5
71	1650.5
72	1250.5
73	968.5
74	738.5
75	585.0
76	445.0
77	281.5
78	184.5
79	121.0
80	78.5
81	54.5
82	39.5
83	24.0
84	16.5
85	13.0
86	9.5
87	6.0
88	5.0
89	4.5
90	3.0
91	4.5
92	3.0
93	1.0
94	2.0
95	1.0
96	0.5
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2559611072905525
2	0.07912059302703728
3	0.0012203690441188269
4	0.0012881673243476505
5	2.7119312091529484E-4
6	6.101845220594134E-4
7	0.0
8	7.457810825170609E-4
9	0.0
10-11	3.615908278870598E-4
12-13	0.0016045592987488277
14-15	4.5198853485882476E-5
16-17	7.909799360029433E-5
18-19	0.0011412710505185326
20-21	0.0013785650313194154
22-23	2.2599426742941238E-5
24-25	2.2599426742941238E-5
26-27	1.9209512731500054E-4
28-29	0.0016271587254917692
30-31	0.001988749553378829
32-33	0.0035029111451558917
34-35	0.004406888214873541
36-37	0.0017288561458350048
38-39	0.003378614298069715
40-41	0.006881525443225608
42-43	0.01331106235159239
44-45	0.0085764824489462
46-47	0.006632931749053253
48-49	0.006700730029282078
50-51	0.005389963278191485
52-53	0.00364980741898501
54-55	0.005954948946765016
56-57	0.0038419025463000103
58-59	0.0021017466870935355
60-61	0.00875727786288973
62-63	0.011796900759815326
64-65	0.007627306525742668
66-67	0.008463485315231493
68-69	0.015096417064284747
70-71	0.004531185061959719
72-73	0.013435359198678566
74-75	0.007988897353629728
76-77	0.004632882482302954
78-79	0.01604559298748828
80-81	0.006644231462424724
82-83	0.015604904166000924
84-85	0.02426048460854742
86-87	0.015932595853773573
88-89	0.016644477796176223
90-91	0.031232407758744793
92-93	0.024249184895175947
94-95	0.02943575333268096
96-97	0.0185767287826977
98-99	0.017650152286237106
100	0.01954850413264417
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4424891.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.1938801888549
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.76338929998037	44.08965599624629
2	16.184966146498002	18.837319615799544
3	4.443388595632481	7.757340707002824
4	1.573387838961013	3.6624617356439395
5	0.7163199436845611	2.0842718489833314
6	0.35216760013118936	1.2296399477058597
7	0.21985825063864073	0.8956083287337402
8	0.1369546197847287	0.6375936588050142
9	0.10102692492881592	0.5291233888640356
>10	0.41029182295303535	4.375389888685696
>50	0.04120922628452047	1.697521006453745
>100	0.0470304792276813	5.814309345161674
>500	0.006620702681185838	2.6479048131213485
>1k	0.0031278910281281654	3.2950005430292606
>5k	5.2131517135469415E-5	0.2532865570448136
>10k+	2.0852606854187766E-4	2.1935726187186844
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25371	0.5733700558951621	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20773	0.4694578917311183	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15992	0.36141003247311626	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10170	0.22983616997571238	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	8349	0.1886826138768164	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4533	0.10244320142575264	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	2.2599426742941238E-5	0.0	0.0	0.0
8	0.0	2.2599426742941238E-5	0.0	0.0	0.0
9	0.0	2.2599426742941238E-5	0.0	2.2599426742941238E-5	0.0
10-11	0.0	2.2599426742941238E-5	0.0	2.2599426742941238E-5	0.0
12-13	0.0	2.2599426742941238E-5	0.0	2.2599426742941238E-5	0.0
14-15	0.0	2.2599426742941238E-5	0.0	2.2599426742941238E-5	0.0
16-17	0.0	2.2599426742941238E-5	0.0	2.2599426742941238E-5	0.0
18-19	0.0	2.2599426742941238E-5	0.0	3.3899140114411855E-5	0.0
20-21	0.0	2.2599426742941238E-5	0.0	4.5198853485882476E-5	0.0
22-23	2.2599426742941238E-5	2.2599426742941238E-5	0.0	1.9209512731500054E-4	1.1299713371470619E-5
24-25	2.2599426742941238E-5	2.2599426742941238E-5	0.0	4.180893947444129E-4	2.2599426742941238E-5
26-27	2.2599426742941238E-5	2.2599426742941238E-5	0.0	7.796802226314728E-4	2.2599426742941238E-5
28-29	2.2599426742941238E-5	2.2599426742941238E-5	0.0	0.0020678475469791233	2.2599426742941238E-5
30-31	2.2599426742941238E-5	2.2599426742941238E-5	0.0	0.006768528309510901	2.2599426742941238E-5
32-33	2.2599426742941238E-5	2.2599426742941238E-5	0.0	0.015729201013087103	2.2599426742941238E-5
34-35	2.2599426742941238E-5	2.2599426742941238E-5	0.0	0.02783119403393214	2.2599426742941238E-5
36-37	2.2599426742941238E-5	2.2599426742941238E-5	0.0	0.04667911593754513	2.2599426742941238E-5
38-39	2.2599426742941238E-5	2.2599426742941238E-5	0.0	0.07879290133926463	2.2599426742941238E-5
40-41	2.2599426742941238E-5	2.2599426742941238E-5	0.0	0.11720062708889326	2.2599426742941238E-5
42-43	2.2599426742941238E-5	2.2599426742941238E-5	0.0	0.1519472457061654	2.2599426742941238E-5
44-45	2.2599426742941238E-5	2.2599426742941238E-5	0.0	0.191530141646427	2.2599426742941238E-5
46-47	2.2599426742941238E-5	2.2599426742941238E-5	0.0	0.23803976188340004	2.2599426742941238E-5
48-49	2.2599426742941238E-5	2.2599426742941238E-5	0.0	0.28826698781958693	2.2599426742941238E-5
50-51	2.2599426742941238E-5	2.2599426742941238E-5	0.0	0.34195192604744384	2.2599426742941238E-5
52-53	2.2599426742941238E-5	2.2599426742941238E-5	0.0	0.39494758175964106	2.2599426742941238E-5
54-55	2.2599426742941238E-5	2.2599426742941238E-5	0.0	0.4585197691875348	2.2599426742941238E-5
56-57	2.2599426742941238E-5	2.2599426742941238E-5	0.0	0.529979156548715	2.2599426742941238E-5
58-59	2.2599426742941238E-5	2.2599426742941238E-5	0.0	0.6041391754056766	2.2599426742941238E-5
60-61	4.5198853485882476E-5	2.2599426742941238E-5	0.0	0.6777455083074363	2.2599426742941238E-5
62-63	6.779828022882371E-5	2.2599426742941238E-5	0.0	0.7523462159858854	2.2599426742941238E-5
64-65	6.779828022882371E-5	2.2599426742941238E-5	0.0	0.8299300479944025	2.2599426742941238E-5
66-67	6.779828022882371E-5	2.2599426742941238E-5	0.0	0.9129829412747116	2.2599426742941238E-5
68-69	6.779828022882371E-5	2.2599426742941238E-5	0.0	1.0100926779891302	2.2599426742941238E-5
70-71	7.909799360029433E-5	2.2599426742941238E-5	0.0	1.1202083847941111	2.2599426742941238E-5
72-73	9.039770697176495E-5	2.2599426742941238E-5	0.0	1.222143099118148	2.2599426742941238E-5
74-75	9.039770697176495E-5	2.2599426742941238E-5	0.0	1.325942266148477	2.2599426742941238E-5
76-77	9.039770697176495E-5	2.2599426742941238E-5	0.0	1.43392232712625	2.2599426742941238E-5
78-79	9.039770697176495E-5	2.2599426742941238E-5	0.0	1.5563886206462487	2.2599426742941238E-5
80-81	9.039770697176495E-5	2.2599426742941238E-5	0.0	1.6857251398961015	6.779828022882371E-5
82-83	9.039770697176495E-5	2.2599426742941238E-5	0.0	1.822044882009523	1.129971337147062E-4
84-85	9.039770697176495E-5	2.2599426742941238E-5	0.0	1.9532684533924112	1.129971337147062E-4
86-87	9.039770697176495E-5	2.2599426742941238E-5	0.0	2.091418749072011	1.129971337147062E-4
88	9.039770697176495E-5	2.2599426742941238E-5	0.0	2.2016361532973354	1.129971337147062E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	7265	0.0	65.952576	2
GTACCTG	7725	0.0	62.48863	1
ACCTGGG	7835	0.0	59.133232	3
TATAACG	715	0.0	54.58398	2
TAACGCA	735	0.0	53.701405	4
CCTGGGG	4805	0.0	38.04082	4
GTACCCG	400	0.0	36.534336	1
TATCACG	455	0.0	29.969517	2
GTACACG	580	0.0	28.447203	1
GTATAAC	1910	0.0	27.642935	1
GGTATCA	19365	0.0	26.534353	1
ATAACGC	1525	0.0	26.498562	3
CCTGGGA	7060	0.0	26.489397	4
GTATCAA	27600	0.0	25.722633	1
TACCCGG	550	0.0	25.647894	2
ATCACGC	570	0.0	25.555275	3
CATGGGG	7780	0.0	25.06468	4
GGGTATG	1620	0.0	23.785223	7
GGATGAC	3115	0.0	22.929466	8
GTACATG	29145	0.0	22.919508	1
>>END_MODULE