FastQCFastQC Report
Mon 6 Jun 2016
ERR1211580_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211580_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5199094
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182120.35029180084068495No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157650.3032259082063144No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105100.2021506054708763No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57340.11028844641008606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92450.048.1122471
TACCTGG54000.040.075582
GTACCTG58800.037.7026141
GTATCAA154000.036.968981
ACCTGGG68250.030.8435483
TCAACGC188300.029.6704884
CAACGCA190250.029.412665
ATCAACG190800.029.3306963
AACGCAG196200.028.7086726
TATCAAC198850.028.0591722
GTACATG192150.026.609691
TACATGG198250.025.4388432
TATAACG4650.025.2931422
ACGCAGA222600.025.1141747
ACATGGG197900.024.6694093
CGCAGAG231500.023.9060658
ATAACGC6100.023.879263
CATGGGG131850.023.627984
TAACGCA6300.021.6297054
GCAGAGT254200.021.2166089
Produced by FastQC (version 0.11.5)