FastQCFastQC Report
Mon 6 Jun 2016
ERR1211580_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211580_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5199094
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209110.402204691817459No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183110.35219597876091485No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126550.24340779374252514No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59640.11471229410354958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97000.046.611651
GTACCTG58950.038.6287731
TACCTGG58450.038.2966542
GTATCAA161800.037.2100221
TATAACG5300.031.9422172
ACCTGGG72000.030.4766773
TCAACGC204500.028.9967484
CAACGCA207000.028.6468225
ATCAACG207050.028.594243
GTACATG195450.028.5121121
TATCAAC212450.028.0451832
AACGCAG215650.027.6072376
TAACGCA6850.027.4380134
TACATGG201500.027.3754562
ACATGGG202400.026.1403353
CATGGGG130150.025.741194
ATAACGC6900.024.5152683
ACGCAGA244200.024.1871917
CGCAGAG252250.023.3221688
CCTGGGG69800.022.8879414
Produced by FastQC (version 0.11.5)