FastQCFastQC Report
Mon 6 Jun 2016
ERR1211579_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211579_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8302503
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227010.2734235687719715No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195090.23497733153483957No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127200.1532068100427064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118650.047.3053321
GTATCAA207650.038.0464131
TCAACGC243050.031.5884284
CAACGCA244750.031.4239625
ATCAACG245900.031.202083
AACGCAG255350.030.3375516
TATCAAC262000.029.4103322
GTACATG272200.029.249281
GTACCTG73650.028.754411
TACCTGG73800.027.600622
TACATGG282150.027.5600592
ACATGGG275150.027.1337783
ACGCAGA290750.026.4339437
CGCAGAG300500.025.5301348
CATGGGG177800.024.92024
GCAGAGT341550.021.9664279
ACCTGGG91450.021.8867763
ATGGGGG109400.021.7304215
GAGTACT204000.020.45213712-13
CAGAGTA329700.019.09516710-11
Produced by FastQC (version 0.11.5)