FastQCFastQC Report
Mon 6 Jun 2016
ERR1211579_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211579_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8302503
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269000.32399867847081776No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232260.27974696305439456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163240.1966154062214732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA137600.046.2176321
GTATCAA233300.037.1200561
TCAACGC287700.029.4490384
GTACCTG69700.029.2866271
ATCAACG289050.029.2627263
CAACGCA290450.029.1693345
TATCAAC301500.028.4011652
TACCTGG70600.028.1740442
AACGCAG301950.028.1673346
GTACATG286300.027.6467151
TACATGG291400.026.6261222
ACATGGG288750.025.8755953
TAACGCA10000.024.9052624
CATGGGG179450.024.5626144
ACGCAGA344350.024.5488197
TATAACG9500.023.7591632
CGCAGAG355100.023.6997878
ACCTGGG89700.021.688183
ATGGGGG106300.021.3066865
GCAGAGT399500.020.818449
Produced by FastQC (version 0.11.5)