FastQCFastQC Report
Mon 6 Jun 2016
ERR1211578_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211578_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6801944
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221720.325965635706498No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197020.2896524875829616No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130660.19209214306968714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107150.047.5314061
GTATCAA185450.037.9903071
GTACCTG62800.034.917441
TACCTGG62150.033.989332
TCAACGC222700.030.6558954
CAACGCA223950.030.3555555
ATCAACG224350.030.3471093
AACGCAG231950.029.4068326
TATCAAC237100.029.0500552
ACCTGGG73300.027.6920283
GTACATG212400.027.6194461
TACATGG218650.026.3371852
ACGCAGA264750.025.586177
CGCAGAG268000.025.0838178
ACATGGG218600.025.0624433
CATGGGG134700.024.4871444
GCAGAGT300550.021.8201359
ATGGGGG87400.020.9639855
GAGTACT194600.019.93082612-13
AGAGTAC272750.019.72320210-11
Produced by FastQC (version 0.11.5)