FastQCFastQC Report
Mon 6 Jun 2016
ERR1211578_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211578_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6801944
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263550.3874627606460741No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238660.3508702806138951No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161220.23702047532293707No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70200.10320578940373516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117500.052.1263051
GTATCAA201200.037.44851
TACCTGG61400.033.7047962
GTACCTG64500.032.8956451
TCAACGC252850.029.1593424
CAACGCA256050.028.8491295
ATCAACG256250.028.7549533
TATCAAC265550.027.8782712
AACGCAG268100.027.6229936
GTACATG224950.027.3323651
ACCTGGG73900.027.2163493
TACATGG233100.025.8270662
ACATGGG227100.025.472493
TAACGCA7400.024.7657014
CATGGGG146750.024.2081414
ACGCAGA307100.023.9769637
CGCAGAG314150.023.3192188
ATGGGGG90400.021.8835935
TATAACG6950.021.6557182
GCAGAGT350150.020.6529969
Produced by FastQC (version 0.11.5)