FastQCFastQC Report
Mon 6 Jun 2016
ERR1211577_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211577_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5076394
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183940.3623438212242785No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154960.30525605380512233No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105980.20877024123817026No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61840.1218187555969848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85150.046.2315031
GTATCAA147500.038.4192281
GTACCTG51850.037.6433071
TACCTGG52150.036.803932
TCAACGC175950.030.8698484
ACCTGGG62900.030.476633
ATCAACG178950.030.4567583
CAACGCA179250.030.3241635
AACGCAG185000.029.4552276
TATCAAC188150.029.2029632
GTACATG168100.028.6908951
TACATGG173200.027.4594352
ACATGGG170500.026.8956993
ACGCAGA207400.026.0706147
CATGGGG102400.025.282364
CGCAGAG212600.025.2790228
ATGGGGG65450.022.0365375
GCAGAGT236350.021.8642489
GAGTACT153650.020.55078112-13
AGAGTAC218250.019.83812510-11
Produced by FastQC (version 0.11.5)