FastQCFastQC Report
Mon 6 Jun 2016
ERR1211577_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211577_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5076394
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217260.4279809644405064No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190080.3744390210846518No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136550.2689901532465762No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68440.13482011049575743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104450.044.7818371
TACCTGG52200.040.9879652
GTACCTG55600.040.5370181
GTATCAA173550.036.270651
ACCTGGG63150.033.4823953
TCAACGC218500.028.3203664
CAACGCA220300.028.1316265
ATCAACG220200.028.0812193
TATCAAC225800.027.4894122
AACGCAG226200.027.3989476
GTACATG188400.026.504141
TAACGCA5150.026.4578954
TACATGG195350.024.8898662
ACATGGG189500.024.696053
CATGGGG107350.024.422874
ACGCAGA256150.024.0297937
CGCAGAG262500.023.4127038
TATAACG5250.023.2878552
CCTGGGG56700.022.5398184
ATGGGGG64050.021.273725
Produced by FastQC (version 0.11.5)