FastQCFastQC Report
Mon 6 Jun 2016
ERR1211576_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211576_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9966243
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT287430.28840356391069333No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249030.24987349796708752No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160260.16080282208651744No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA151700.049.256311
GTATCAA260100.037.6331021
TCAACGC310450.030.7116624
CAACGCA313350.030.3793955
ATCAACG316150.030.0842343
AACGCAG330900.028.835396
GTACATG336700.028.3569581
TATCAAC337750.028.3186052
TACATGG347750.026.7331182
ACATGGG345600.025.8209883
ACGCAGA375600.025.2537357
CGCAGAG388600.024.2893568
CATGGGG222100.023.0193614
GTACCTG85100.021.7848831
TACCTGG80950.021.213182
GCAGAGT434350.021.1576719
GAGTACT264950.020.04893312-13
AGAGTAC392600.019.5798310-11
CAGAGTA416750.019.29643810-11
GAGTACA246700.018.8156071
Produced by FastQC (version 0.11.5)