FastQCFastQC Report
Mon 6 Jun 2016
ERR1211576_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211576_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9966243
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT343850.3450146660080433No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302720.3037453531887593No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201840.20252365911607814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA157350.048.4240261
GTATCAA276050.039.7461361
TCAACGC343800.031.2984584
ATCAACG347350.030.9379983
CAACGCA348200.030.902655
AACGCAG363300.029.6968736
TATCAAC367450.029.5632022
GTACATG345100.027.8858781
TACATGG353700.026.4579242
ACGCAGA420700.025.522067
ACATGGG358450.025.3787443
CGCAGAG434150.024.6989158
CATGGGG222700.023.4837954
TACCTGG77950.022.5628132
GTACCTG83000.021.9846291
GCAGAGT484450.021.6783549
GAGTACT297950.020.26540412-13
AGAGTAC443500.019.8605710-11
CAGAGTA468050.018.89916410-11
AGTACTT323350.018.59357612-13
Produced by FastQC (version 0.11.5)