FastQCFastQC Report
Mon 6 Jun 2016
ERR1211575_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211575_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7030400
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225450.3206787664997724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196230.2791164087391898No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124680.17734410559854347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107450.049.6552121
GTATCAA191850.040.646181
TCAACGC231900.032.5590364
ATCAACG235650.032.1428873
CAACGCA235400.032.01215
AACGCAG243000.031.143136
TATCAAC252700.030.3685112
GTACATG236800.028.7072071
ACGCAGA279100.026.8963287
TACATGG244700.026.861932
ACATGGG244250.026.2786733
CGCAGAG285500.026.1628848
TACCTGG55100.025.4471662
GTACCTG58050.025.1923181
CATGGGG141600.024.0232474
GCAGAGT322050.022.5809529
ATGGGGG81800.020.8482485
GAGTACT203800.020.44939412-13
AGAGTAC294650.020.35433210-11
CAGAGTA309600.019.75843610-11
Produced by FastQC (version 0.11.5)