FastQCFastQC Report
Mon 6 Jun 2016
ERR1211575_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211575_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7030400
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265120.3771051433773327No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237790.3382311106053709No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161800.23014337733272644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123750.050.818661
GTATCAA215650.038.562191
TCAACGC266600.030.3848614
ATCAACG269300.030.0634173
CAACGCA270050.030.031485
AACGCAG280500.028.9304436
TATCAAC286200.028.5079082
GTACATG253600.027.3820571
TACATGG261750.026.068512
TACCTGG55850.025.7652132
ACATGGG261350.025.350473
GTACCTG58750.025.2764031
ACGCAGA320800.025.1787417
CGCAGAG328150.024.5288648
CATGGGG150550.022.6899624
GCAGAGT361400.022.1941189
AGAGTAC334750.020.42379610-11
GAGTACT229550.020.3572212-13
AGTACTT242050.019.7524212-13
CAGAGTA352650.019.65360310-11
Produced by FastQC (version 0.11.5)