FastQCFastQC Report
Mon 6 Jun 2016
ERR1211574_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211574_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8842731
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234020.2646467477072411No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185880.2102065527041363No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116450.13169008533675852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120800.048.095281
GTATCAA210350.038.583091
TCAACGC248200.031.6939744
ATCAACG251400.031.347693
CAACGCA249500.031.3367675
AACGCAG261150.029.9877096
TATCAAC271950.029.1837182
GTACATG278450.029.1638321
TACATGG283250.027.8533692
ACATGGG287700.026.5430763
ACGCAGA298800.025.9735957
CGCAGAG309450.024.7926468
CATGGGG180650.024.6860284
TACCTGG71950.024.1273822
GTACCTG76650.023.6868441
GCAGAGT346350.021.5816489
ATGGGGG105050.019.8590455
AGAGTAC315450.019.50452210-11
GAGTACT215000.019.43933312-13
CAGAGTA338000.019.09291810-11
Produced by FastQC (version 0.11.5)