FastQCFastQC Report
Mon 6 Jun 2016
ERR1211574_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211574_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8842731
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264480.29909311953513No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220820.24971923266692156No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147370.16665665844635555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA133050.048.56951
GTATCAA232650.037.5821721
TCAACGC279750.030.5877694
CAACGCA282050.030.3379965
ATCAACG283850.030.0966283
AACGCAG292600.029.3568746
TATCAAC300950.028.6261772
GTACATG301450.027.9102651
TACATGG309000.026.7998982
ACATGGG311100.025.8743953
ACGCAGA340200.025.1386857
CATGGGG187200.024.599614
CGCAGAG350350.024.3567438
GTACCTG74100.022.008931
ATGGGGG104200.021.8263285
TACCTGG71650.021.5929472
GCAGAGT393350.021.2639489
TATAACG7650.020.9001642
TAACGCA7550.020.5387944
GAGTACT237350.019.6690312-13
Produced by FastQC (version 0.11.5)