FastQCFastQC Report
Mon 6 Jun 2016
ERR1211573_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211573_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9597198
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228220.23779857412548955No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195880.20410123871571684No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126080.13137167744168662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA143100.041.821371
GTATCAA232850.035.463871
GTACATG289700.030.0021971
TCAACGC273950.029.1415944
ATCAACG277700.028.7471733
CAACGCA277900.028.657215
TACATGG296250.028.4289742
AACGCAG288700.027.613696
ACATGGG296850.027.4308433
TATCAAC298050.027.0101742
CATGGGG187450.024.2650474
ACGCAGA327850.024.087467
CGCAGAG338550.023.174378
GTACCTG75350.020.2160131
GCAGAGT381900.019.9778339
ATGGGGG107700.019.9366995
TACCTGG74900.019.7248782
GAGTACT227900.019.40115212-13
AGAGTAC329150.019.01404210-11
GAGTACA212150.018.6507021
Produced by FastQC (version 0.11.5)