FastQCFastQC Report
Mon 6 Jun 2016
ERR1211573_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211573_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9597198
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT273190.2846560006368526No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234900.24475893901532508No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159870.1665798704997021No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA170750.040.3790131
GTATCAA269800.033.315781
TCAACGC320050.027.3813344
ATCAACG322800.027.1340773
GTACATG307400.027.1236061
CAACGCA324100.027.0101785
AACGCAG337050.025.973086
TACATGG314650.025.8874682
TATCAAC344500.025.6375052
ACATGGG318950.024.4708863
CATGGGG193900.023.3368434
ACGCAGA386800.022.5107177
CGCAGAG397650.021.7783378
GTACCTG72250.021.5399721
ATGGGGG112900.020.3104485
GAGTACT258600.019.27877212-13
GCAGAGT446150.019.2211849
TACCTGG76850.019.1544552
AGAGTAC388050.018.85986110-11
TATAACG9650.018.5193482
Produced by FastQC (version 0.11.5)