FastQCFastQC Report
Mon 6 Jun 2016
ERR1211572_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211572_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5273100
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175860.3335040109233658No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154260.2925413893155829No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101870.19318806774003908No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71790.13614382431586733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83750.050.2379041
TACCTGG56300.042.364992
GTACCTG59350.041.009121
GTATCAA138750.036.5109181
ACCTGGG68550.033.8632243
TCAACGC169400.029.3207114
ATCAACG171800.028.9108353
CAACGCA172600.028.8554975
AACGCAG179750.027.8608346
TATCAAC182300.027.4059852
GTACATG164050.026.3085161
TATAACG4700.025.0235962
TACATGG167850.024.720362
ACGCAGA202350.024.6794747
CGCAGAG208750.023.67558
ACATGGG168200.023.606933
TAACGCA5300.023.0520944
CCTGGGG66050.021.8413014
CATGGGG99300.021.2476064
GCAGAGT232750.020.5683089
Produced by FastQC (version 0.11.5)