FastQCFastQC Report
Mon 6 Jun 2016
ERR1211572_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211572_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5273100
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211310.4007320172194724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188740.35792987047467334No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130150.2468187593635622No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75050.1423261459103753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97650.049.6447871
GTACCTG56000.043.3682561
TACCTGG55550.042.9260562
GTATCAA164750.037.182321
ACCTGGG69950.033.655153
TCAACGC204300.029.233364
ATCAACG205050.029.1035183
CAACGCA206250.029.0022625
TATCAAC213550.028.1687812
AACGCAG213900.028.075126
TATAACG5350.027.2524152
GTACATG178700.026.6004331
TACATGG185900.024.6926382
ACGCAGA244500.024.561197
ACATGGG182800.024.1888393
CGCAGAG251300.023.7469948
CCTGGGG63500.023.0877954
TAACGCA6600.022.7828254
CATGGGG108150.022.4628874
GCAGAGT277900.021.2877869
Produced by FastQC (version 0.11.5)