##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211572_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5273100 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01001839525137 33.0 31.0 34.0 30.0 34.0 2 32.26096565587605 34.0 31.0 34.0 30.0 34.0 3 32.35268608598358 34.0 31.0 34.0 30.0 34.0 4 35.99665661565303 37.0 35.0 37.0 35.0 37.0 5 35.89187498814739 37.0 35.0 37.0 35.0 37.0 6 35.92423489029224 37.0 35.0 37.0 35.0 37.0 7 35.83116762435759 37.0 35.0 37.0 35.0 37.0 8 35.813040905729075 37.0 35.0 37.0 35.0 37.0 9 37.44547268210351 39.0 37.0 39.0 34.0 39.0 10-11 37.40056731334509 39.0 37.0 39.0 34.0 39.0 12-13 37.305108949194974 39.0 37.0 39.0 34.0 39.0 14-15 38.52381398039104 40.0 38.0 41.0 34.0 41.0 16-17 38.45174925565607 40.0 38.0 41.0 34.0 41.0 18-19 38.44913409569324 40.0 38.0 41.0 34.0 41.0 20-21 38.385083726839994 40.0 38.0 41.0 34.0 41.0 22-23 38.26791403538715 40.0 38.0 41.0 34.0 41.0 24-25 38.154895412565665 40.0 37.5 41.0 33.0 41.0 26-27 37.94372958980486 40.0 37.0 41.0 33.0 41.0 28-29 37.953630976086174 40.0 37.0 41.0 33.0 41.0 30-31 37.833336841706014 40.0 37.0 41.0 33.0 41.0 32-33 37.63764540782462 40.0 37.0 41.0 32.5 41.0 34-35 37.4795101553166 40.0 36.0 41.0 32.0 41.0 36-37 37.37216523487133 40.0 36.0 41.0 32.0 41.0 38-39 37.290792133659515 40.0 36.0 41.0 31.0 41.0 40-41 37.13221975687925 39.5 36.0 41.0 31.0 41.0 42-43 36.938367848134874 39.0 36.0 41.0 30.5 41.0 44-45 36.73288852856953 39.0 35.0 41.0 30.0 41.0 46-47 36.47603525440443 39.0 35.0 41.0 29.5 41.0 48-49 36.220614439324116 39.0 35.0 40.0 28.5 41.0 50-51 36.00461995789953 39.0 35.0 40.0 28.0 41.0 52-53 35.79380980827217 38.5 35.0 40.0 27.0 41.0 54-55 35.570785211735036 38.0 34.0 40.0 27.0 41.0 56-57 35.34997288122736 38.0 34.0 40.0 26.0 41.0 58-59 35.07706538848116 38.0 34.0 40.0 26.0 41.0 60-61 34.75621456069485 37.5 33.0 40.0 26.0 41.0 62-63 34.41249597011246 37.0 33.0 40.0 25.0 41.0 64-65 34.083143217462215 36.5 32.5 39.5 24.5 41.0 66-67 33.71881881625609 36.0 32.0 39.0 23.0 40.5 68-69 33.32072025942993 35.5 31.5 39.0 22.5 40.0 70-71 32.9407130530428 35.0 31.0 38.0 22.0 40.0 72-73 32.52152471980429 35.0 31.0 37.5 21.5 40.0 74-75 32.12741603610779 35.0 30.5 37.0 20.5 39.0 76-77 30.657614780679296 33.5 28.5 35.5 20.0 37.5 78-79 31.519689556427906 34.0 30.0 36.0 20.0 38.5 80-81 31.47837856289469 34.0 30.0 36.0 20.0 37.5 82-83 31.200032997667407 34.0 30.0 35.0 20.0 37.0 84-85 30.874721511065598 34.0 30.0 35.0 19.5 36.5 86-87 30.569109537084447 34.0 29.0 35.0 19.0 36.0 88-89 30.283863097608616 34.0 29.0 35.0 18.0 36.0 90-91 30.001387608806965 34.0 29.0 35.0 18.0 35.5 92-93 29.759171170658625 34.0 29.0 35.0 17.0 35.0 94-95 29.5347283381692 34.0 29.0 35.0 16.5 35.0 96-97 29.294093607176045 33.0 27.0 35.0 15.0 35.0 98-99 29.082262141814113 33.0 27.0 35.0 13.5 35.0 100 27.322969031499497 31.0 24.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22008348210111706 1101 2 0.11586692929248699 1101 3 0.11190714073254071 1101 4 0.11780239609737464 1101 5 0.09235459155530634 1101 6 0.03685533488871329 1101 7 0.05822558576888781 1101 8 0.048171806495361125 1101 9 0.03915248052435061 1101 10-11 0.12309893333988953 1101 12-13 0.08774084686788086 1101 14-15 0.0902638471930004 1101 16-17 0.0730417541040822 1101 18-19 0.12990875649749967 1101 20-21 -0.013114565684823276 1101 22-23 -0.09304885116872441 1101 24-25 -0.13021458962565902 1101 26-27 -0.11129194811945098 1101 28-29 0.03006946876747918 1101 30-31 0.09576508378217596 1101 32-33 0.03548983724910926 1101 34-35 -0.007987783632984247 1101 36-37 -0.13510904449119465 1101 38-39 -0.14202908949286552 1101 40-41 -0.12179967294762406 1101 42-43 -0.05916065859224773 1101 44-45 -0.06668755339504884 1101 46-47 -0.2764060610641792 1101 48-49 -0.17603784390795596 1101 50-51 -0.21378984066416962 1101 52-53 -0.2725369887563218 1101 54-55 -0.3925713706122096 1101 56-57 -0.41675455416002194 1101 58-59 -0.4302579594956484 1101 60-61 -0.4169356613744384 1101 62-63 -0.36837260589415877 1101 64-65 -0.4517949581066816 1101 66-67 -0.49628883143773095 1101 68-69 -0.49905478975376383 1101 70-71 -0.6760833869926657 1101 72-73 -0.6412357174096108 1101 74-75 -0.8425789393445626 1101 76-77 -0.7046619974727335 1101 78-79 -0.6958015711058998 1101 80-81 -0.6003109945220046 1101 82-83 -0.6994039964327357 1101 84-85 -0.863160783422348 1101 86-87 -0.896162648275336 1101 88-89 -0.8835203164612508 1101 90-91 -1.0454216561146623 1101 92-93 -1.0812057606860286 1101 94-95 -1.1891566255895576 1101 96-97 -1.2380117222416587 1101 98-99 -1.304911569198591 1101 100 -1.3450764995917055 1102 1 0.23795496689432838 1102 2 0.1572608662556121 1102 3 0.07672017010021648 1102 4 0.08761592227949677 1102 5 0.0589442042342867 1102 6 0.11387596868978989 1102 7 0.10682298285248493 1102 8 0.08129276970328192 1102 9 0.062336875268094616 1102 10-11 0.11958423315238065 1102 12-13 0.21664361783474106 1102 14-15 0.0888313655342472 1102 16-17 0.11916021479263605 1102 18-19 0.028455507855071005 1102 20-21 0.06132993497053718 1102 22-23 0.015733144275671407 1102 24-25 0.01870777721234873 1102 26-27 0.06405392487577188 1102 28-29 0.03262635122676727 1102 30-31 0.07399940523355752 1102 32-33 0.05433877477193505 1102 34-35 3.1698345187436416E-4 1102 36-37 -0.04319788246106526 1102 38-39 0.03798037583556635 1102 40-41 1.8294790999817678E-4 1102 42-43 -0.07475647467391155 1102 44-45 -0.03391184478461895 1102 46-47 -0.08783038965544421 1102 48-49 -0.08442791494194068 1102 50-51 -0.12228699582498592 1102 52-53 -0.10898815301408149 1102 54-55 -0.2446501442812945 1102 56-57 -0.26229207734644433 1102 58-59 -0.27001286995794516 1102 60-61 -0.252743412490382 1102 62-63 -0.2727533983069108 1102 64-65 -0.33338031746703933 1102 66-67 -0.33762128499255795 1102 68-69 -0.42119411329512246 1102 70-71 -0.3721601180626095 1102 72-73 -0.42271635443334077 1102 74-75 -0.40127040415619675 1102 76-77 -0.312029958377142 1102 78-79 -0.35758500360880063 1102 80-81 -0.3956297656252623 1102 82-83 -0.5002548418348383 1102 84-85 -0.531049303850228 1102 86-87 -0.4849244539072721 1102 88-89 -0.543921647504721 1102 90-91 -0.4910770731599925 1102 92-93 -0.7145648734019758 1102 94-95 -0.6914776434680299 1102 96-97 -0.7811590788044782 1102 98-99 -0.7670857871420722 1102 100 -0.6499662158680302 1103 1 0.2869810314045722 1103 2 0.18040485324731748 1103 3 0.1474301686305992 1103 4 0.1204042224926738 1103 5 0.1534941883314147 1103 6 0.13336542414097607 1103 7 0.14374533515935184 1103 8 0.13455265746299716 1103 9 0.10704774165800046 1103 10-11 0.13223772998011896 1103 12-13 0.149373985399464 1103 14-15 0.20331892328172074 1103 16-17 0.13676763292587424 1103 18-19 0.13510209254826577 1103 20-21 0.15251473778914004 1103 22-23 0.040633646108240384 1103 24-25 0.02247406009797004 1103 26-27 0.0962763741439332 1103 28-29 0.11654914233198355 1103 30-31 0.18964951081571968 1103 32-33 0.20826000375783593 1103 34-35 0.24506531358040462 1103 36-37 0.15663849145243347 1103 38-39 0.1432815105003371 1103 40-41 0.09053572744954153 1103 42-43 0.06277456862217434 1103 44-45 0.14890153210350832 1103 46-47 0.057488628702110134 1103 48-49 0.11502284988795708 1103 50-51 0.10118483104712794 1103 52-53 -0.032059079977493354 1103 54-55 -0.03890426812675685 1103 56-57 -0.06506698478600015 1103 58-59 -0.07533489779469704 1103 60-61 -0.1485654770849152 1103 62-63 -0.25206547184087214 1103 64-65 -0.16410726700897982 1103 66-67 -0.1766666242190098 1103 68-69 -0.2535448218789824 1103 70-71 -0.29147352558803163 1103 72-73 -0.27615859493252515 1103 74-75 -0.21448989798641804 1103 76-77 -0.1529305577204525 1103 78-79 -0.11203123613659116 1103 80-81 -0.18541719151066616 1103 82-83 -0.18892703554764978 1103 84-85 -0.22705980691176464 1103 86-87 -0.2947822961809301 1103 88-89 -0.20312029768340167 1103 90-91 -0.22762947582956272 1103 92-93 -0.28954084521094003 1103 94-95 -0.3749647451684659 1103 96-97 -0.4232221459986327 1103 98-99 -0.39506959326456226 1103 100 -0.38581862077019125 1104 1 0.2709006397108311 1104 2 0.17664785836327468 1104 3 0.16555733343508905 1104 4 0.12697000791038704 1104 5 0.18218660298443723 1104 6 0.14439803126668949 1104 7 0.17685623019483643 1104 8 0.1398586901309784 1104 9 0.15224968997294042 1104 10-11 0.19778171848834347 1104 12-13 0.17032885766236916 1104 14-15 0.16079813261044507 1104 16-17 0.14024801763543593 1104 18-19 0.2383018929686429 1104 20-21 0.03953474856641748 1104 22-23 0.12758758809538762 1104 24-25 0.03280948058132793 1104 26-27 0.12203489687740898 1104 28-29 0.1070110416168859 1104 30-31 0.1255368104710115 1104 32-33 0.04483849420527264 1104 34-35 0.12908471169900082 1104 36-37 0.23115439070286214 1104 38-39 0.18008401478906677 1104 40-41 0.11994396824454867 1104 42-43 0.10896349144670125 1104 44-45 0.1279666171095144 1104 46-47 0.12322617324227281 1104 48-49 0.0725853907843188 1104 50-51 -0.13484283531075647 1104 52-53 -0.07261238910248125 1104 54-55 -0.04123075510540275 1104 56-57 0.02168095171735729 1104 58-59 -0.08881005962976474 1104 60-61 -0.15493597256373448 1104 62-63 -0.10126196722654157 1104 64-65 -0.11382344551554269 1104 66-67 -0.019257211580331557 1104 68-69 -0.21073840471712657 1104 70-71 -0.04505809209620537 1104 72-73 -0.1202458734272227 1104 74-75 -0.1586606953106937 1104 76-77 0.07640958561331246 1104 78-79 0.02855159795580775 1104 80-81 0.028291312585047024 1104 82-83 0.023559168188288737 1104 84-85 -0.17642126273609549 1104 86-87 -0.07866385216543748 1104 88-89 -0.17052449438321204 1104 90-91 -0.14720397090459159 1104 92-93 -0.24054661700569113 1104 94-95 -0.22333532866040073 1104 96-97 -0.3283456884287759 1104 98-99 -0.3126819298876171 1104 100 -0.3228810931945709 1105 1 0.2583062898822348 1105 2 0.20249517101409253 1105 3 0.13638571378795916 1105 4 0.09019038084370834 1105 5 0.10129680842911881 1105 6 0.09181270451370693 1105 7 0.10028161426176041 1105 8 0.06213884805909231 1105 9 0.1652609150717197 1105 10-11 0.11634706093723679 1105 12-13 0.08153243453045889 1105 14-15 0.10565013294878156 1105 16-17 0.15937716398142499 1105 18-19 0.14351459163175662 1105 20-21 0.09465415468716998 1105 22-23 0.048008271789051093 1105 24-25 0.06397654138901032 1105 26-27 0.21382885091066584 1105 28-29 0.12616157829319263 1105 30-31 0.2349718489994146 1105 32-33 0.24263995624446721 1105 34-35 0.2128681388557041 1105 36-37 0.07737205229373245 1105 38-39 0.004851426155248362 1105 40-41 0.09455496197688262 1105 42-43 0.16317191916262175 1105 44-45 0.13976879933481712 1105 46-47 0.198731171443832 1105 48-49 0.1329937078980592 1105 50-51 0.10603676138772755 1105 52-53 0.002644781541853547 1105 54-55 0.0260547387654384 1105 56-57 0.11147129982676063 1105 58-59 0.2234884254928744 1105 60-61 0.19105987400092772 1105 62-63 0.1836999456708881 1105 64-65 0.14318700750703073 1105 66-67 0.05629087093006291 1105 68-69 0.06549918868042681 1105 70-71 0.062177993485491356 1105 72-73 0.1267240462689614 1105 74-75 0.1597887074992883 1105 76-77 0.09409922008529392 1105 78-79 0.10696085644650921 1105 80-81 0.1250331300498857 1105 82-83 0.12759179876604776 1105 84-85 0.09525202554236145 1105 86-87 0.08874825964013056 1105 88-89 0.06387074514141844 1105 90-91 0.1007089789532003 1105 92-93 0.014313888519339457 1105 94-95 -0.027632042096879417 1105 96-97 0.12281546405927912 1105 98-99 0.16822028387998955 1105 100 0.08830897132764193 1106 1 0.2993198417462182 1106 2 0.18075152440501085 1106 3 0.16501426141313402 1106 4 0.12930858913775012 1106 5 0.11570286101000704 1106 6 0.13001276800889627 1106 7 0.12449682706497356 1106 8 0.10494018669804461 1106 9 0.10162313389210453 1106 10-11 0.15542228547138137 1106 12-13 0.18566327125187598 1106 14-15 0.2102715169602618 1106 16-17 0.18167057417820587 1106 18-19 0.16131826045149467 1106 20-21 0.1293607650407793 1106 22-23 0.10585584183172614 1106 24-25 0.1564565228553434 1106 26-27 0.22998636184709653 1106 28-29 0.19732502368309213 1106 30-31 0.21424674543501254 1106 32-33 0.20572740894407104 1106 34-35 0.2638267335153017 1106 36-37 0.2671770543892009 1106 38-39 0.21993466125196903 1106 40-41 0.2588942777254246 1106 42-43 0.2636031657458773 1106 44-45 0.25685412641607286 1106 46-47 0.3257570124426863 1106 48-49 0.29924696970476816 1106 50-51 0.26560242867440564 1106 52-53 0.2865344001554533 1106 54-55 0.2384632574583918 1106 56-57 0.2683774118999054 1106 58-59 0.33553802979067626 1106 60-61 0.3077362547592486 1106 62-63 0.2251621973426836 1106 64-65 0.1988539146641486 1106 66-67 0.24390725619649345 1106 68-69 0.27259361280047045 1106 70-71 0.2933364080872991 1106 72-73 0.17972248654933765 1106 74-75 0.13042950088599525 1106 76-77 0.249942571680986 1106 78-79 0.336525803982461 1106 80-81 0.23662674613043677 1106 82-83 0.20532594817182215 1106 84-85 0.21591430593925764 1106 86-87 0.32681292104513915 1106 88-89 0.2021550035632913 1106 90-91 0.22459625878277123 1106 92-93 0.18922730659799214 1106 94-95 0.31031764099691017 1106 96-97 0.26542571001761317 1106 98-99 0.23315827509889786 1106 100 0.2273122175430018 1107 1 0.22534219901838526 1107 2 0.16053006182723806 1107 3 0.14613320324543366 1107 4 0.09927191463852836 1107 5 0.0421334881279023 1107 6 0.061993801188926057 1107 7 0.07880867292657001 1107 8 0.08216154724142655 1107 9 0.10635306441446346 1107 10-11 0.10253842491352572 1107 12-13 0.1849536774893039 1107 14-15 0.17376354711547748 1107 16-17 0.11139145473000411 1107 18-19 0.16920799914920792 1107 20-21 0.1058321811077434 1107 22-23 0.1437112278499697 1107 24-25 0.17937852310637936 1107 26-27 0.18496895564059201 1107 28-29 0.13688984123419345 1107 30-31 0.17435308610318856 1107 32-33 0.11101543614309861 1107 34-35 0.17941522304420943 1107 36-37 0.12577419567342218 1107 38-39 0.19618381764917814 1107 40-41 0.1832719630530235 1107 42-43 0.15838598381658642 1107 44-45 0.1795801445728955 1107 46-47 0.19644708075941963 1107 48-49 0.2768446502663622 1107 50-51 0.25141424121358824 1107 52-53 0.30711473602884354 1107 54-55 0.28369314986254324 1107 56-57 0.23586725517871798 1107 58-59 0.24383476497142453 1107 60-61 0.25671543957891174 1107 62-63 0.22541136300072395 1107 64-65 0.2817982136457431 1107 66-67 0.3310101758186974 1107 68-69 0.34543860768304313 1107 70-71 0.2243229615426543 1107 72-73 0.31833332392422165 1107 74-75 0.2743678397033307 1107 76-77 0.18014713160932416 1107 78-79 0.26527859814536825 1107 80-81 0.20775820826005287 1107 82-83 0.3553932462169094 1107 84-85 0.2251655835602726 1107 86-87 0.24178712056718155 1107 88-89 0.285892657113056 1107 90-91 0.1260160085719626 1107 92-93 0.12881094345744515 1107 94-95 0.29784077030722145 1107 96-97 0.2842353110400069 1107 98-99 0.24614467277404017 1107 100 0.2290770982330521 1108 1 0.31955501407927045 1108 2 0.20899734215620214 1108 3 0.16352014321451946 1108 4 0.11695979790129485 1108 5 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2316 74-75 -0.2143549500498878 2316 76-77 -0.1087187866329451 2316 78-79 -0.362678415006652 2316 80-81 -0.0793941825566904 2316 82-83 -0.1448917472512221 2316 84-85 0.029997245648448967 2316 86-87 0.18796824967806813 2316 88-89 0.19342899821851844 2316 90-91 0.07797281764313269 2316 92-93 0.21597832046194654 2316 94-95 0.3260912696335616 2316 96-97 0.3268934545056439 2316 98-99 0.32422127154596936 2316 100 0.10818257796379172 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 4.0 10 10.0 11 35.0 12 91.0 13 240.0 14 697.0 15 1749.0 16 4078.0 17 8586.0 18 15187.0 19 23020.0 20 31105.0 21 40829.0 22 51500.0 23 57920.0 24 62451.0 25 68212.0 26 78371.0 27 91957.0 28 108955.0 29 128835.0 30 152998.0 31 181562.0 32 220792.0 33 273004.0 34 354354.0 35 482160.0 36 688934.0 37 1029742.0 38 969688.0 39 146015.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.51931085683758 19.72624772689033 13.21117557199662 23.54326584427547 2 15.422667443329875 21.135602321106962 39.64134938209533 23.800380853467836 3 17.439986072513204 26.91318040153794 30.69176977318147 24.955063752767387 4 11.895678414030916 17.161615090798648 38.04696379186128 32.89574270330915 5 12.340157611720654 37.99811685189979 35.44226956326325 14.219455973116307 6 30.8081046571189 37.56589855581202 18.078207616174797 13.547789170894282 7 27.12222411863989 32.757827463920655 22.536648271415295 17.58330014602416 8 26.274782740620985 35.829056101041616 20.221919847527296 17.674241310810107 9 26.432098765432098 15.243765526919647 20.522235497145893 37.80190021050236 10-11 24.759540462164384 26.31954888608281 28.894836708827032 20.02607394292577 12-13 25.0997836066152 24.10000842967964 29.12228639462033 21.67792156908483 14-15 22.399133792102138 25.516551083148638 26.70582327394195 25.37849185080728 16-17 21.986256083119283 28.399072537360166 27.38394793268054 22.230723446840013 18-19 21.753091928788372 27.366842317913743 29.515079858529518 21.364985894768363 20-21 22.449493955773818 26.58333029110996 29.677257238871746 21.28991851424448 22-23 22.228432990081735 26.51823405586847 29.46578862528683 21.787544328762966 24-25 21.942457367100445 26.699735772076345 29.592806810568128 21.76500005025508 26-27 22.00070452016535 26.777920167340135 29.509524060122217 21.711851252372302 28-29 22.03945893055407 26.694246151835916 29.62923488643043 21.637060031179587 30-31 22.254410296070436 26.72690053976843 29.552264535458168 21.466424628702967 32-33 22.02327128567462 26.886226193528618 29.450006599738742 21.640495921058022 34-35 22.26884465163136 26.92006257330587 29.432790259471364 21.37830251559141 36-37 22.30591693533093 26.835748150957713 29.25696946709653 21.60136544661483 38-39 22.183520291694112 27.079080077247507 29.18751295534297 21.549886675715417 40-41 22.25557488650901 26.909091805634432 29.267972816214154 21.5673604916424 42-43 22.084813830099982 26.94637513319255 29.19577873023544 21.77303230647203 44-45 22.12604457663181 27.195392526017038 28.94958617112968 21.72897672622147 46-47 22.107916618937043 27.0838248536439 28.759864860046626 22.04839366737243 48-49 22.09431345222154 27.175989236015198 28.747601947629597 21.98209536413366 50-51 22.122661320001566 27.433159811368473 28.514298659379484 21.929880209250477 52-53 22.18683803805277 27.45142178960042 28.34150828730487 22.020231885041945 54-55 22.09500115593255 27.462510897912097 28.448619035165194 21.99386891099016 56-57 22.16019868351594 27.509153629348965 28.2644957711669 22.0661519159682 58-59 22.182217506786237 27.409072216055048 28.418063415449623 21.99064686170909 60-61 22.28548122448937 27.58332836274232 28.316513453927932 21.814676958840376 62-63 22.145420499311193 27.565149780745408 28.417540481327396 21.871889238616006 64-65 22.112024788780264 27.31094724001405 28.444691039215524 22.132336931990164 66-67 22.042539923554866 27.45407663915278 28.28615973632337 22.217223700968987 68-69 21.983018516857157 27.484740620420077 28.343639653883645 22.188601208839128 70-71 22.05034294043198 27.332121529239995 28.30004127745228 22.317494252875743 72-73 21.997310338411623 27.554038138722316 28.096930237127783 22.35172128573828 74-75 21.955617240423177 27.605857971983117 28.02454464315856 22.413980144435143 76-77 21.936067567280528 27.52569389445704 28.211736807129657 22.32650173113278 78-79 21.888305499581204 27.51759398633147 28.151895507350172 22.442205006737154 80-81 21.906514944346366 27.75354362285386 27.9054758274459 22.43446560535387 82-83 22.003545499005796 27.706890241930566 27.971906477197837 22.317657781865798 84-85 21.94319946766336 27.738003646536356 27.895348731337677 22.423448154462605 86-87 21.87246166193768 27.69675169544979 27.99890787152829 22.43187877108424 88-89 21.796234276847358 27.616055487600054 28.016833828347377 22.57087640720521 90-91 21.910749825642124 27.763549762206495 27.928692965199282 22.397007446952095 92-93 21.892389977663246 27.679943366640398 27.939427571716667 22.48823908397969 94-95 21.70748488760888 27.580782479073328 27.971240454807756 22.74049217851003 96-97 21.77947731295358 27.625704869948475 27.941059457503236 22.653758359594704 98-99 21.716423508676993 27.567844441099847 27.898001390124538 22.81773066009862 100 20.881798467362746 27.959638010663323 28.14404309183703 23.014520430136898 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1931.0 1 1570.5 2 3378.5 3 4839.0 4 2911.5 5 1963.0 6 2984.0 7 5481.0 8 8382.5 9 9610.5 10 8089.0 11 6189.0 12 6034.0 13 6483.5 14 6966.0 15 7390.0 16 7963.0 17 8444.5 18 8906.5 19 9579.5 20 10445.0 21 11787.0 22 13827.5 23 16883.5 24 21081.5 25 26023.5 26 31972.5 27 38970.5 28 46696.0 29 55377.5 30 65675.5 31 75794.5 32 85731.0 33 97132.5 34 109046.0 35 119398.5 36 132818.5 37 145616.5 38 154346.0 39 162289.0 40 165850.0 41 168892.5 42 172741.5 43 176843.5 44 181174.0 45 187164.0 46 191834.5 47 193407.0 48 195662.0 49 196731.0 50 194845.5 51 188286.0 52 179572.5 53 172683.5 54 165343.0 55 154362.5 56 140509.5 57 125924.0 58 112458.5 59 97554.5 60 80929.0 61 66459.0 62 53803.0 63 41676.0 64 31116.5 65 23171.0 66 17321.5 67 13243.5 68 10289.5 69 7896.0 70 6198.5 71 4856.5 72 3773.5 73 3010.5 74 2368.5 75 1775.5 76 1314.0 77 915.0 78 618.0 79 448.5 80 308.0 81 217.0 82 145.5 83 100.5 84 81.0 85 61.0 86 37.5 87 21.0 88 17.0 89 14.0 90 8.5 91 6.5 92 5.0 93 3.0 94 1.0 95 1.0 96 2.0 97 2.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2509908782310216 2 0.08357512658587928 3 0.0014033490735999696 4 0.0011188864235459218 5 2.844626500540479E-4 6 6.258178301189054E-4 7 0.0 8 4.7410441675674656E-4 9 0.0 10-11 3.698014450702623E-4 12-13 0.001223189395232406 14-15 1.896417667026986E-5 16-17 6.637461834594451E-5 18-19 0.0010714759818702471 20-21 0.001754186341999962 22-23 0.0 24-25 1.896417667026986E-5 26-27 1.4223132502702395E-4 28-29 0.0018679714020215811 30-31 0.0018584893136864465 32-33 0.003110124973924257 34-35 0.004636741195880981 36-37 0.0018964176670269862 38-39 0.003394587623978305 40-41 0.00615387532950257 42-43 0.012478428249037568 44-45 0.008524397413286302 46-47 0.006523676774572832 48-49 0.006286624566194458 50-51 0.005319451556010695 52-53 0.003517854772335059 54-55 0.005708217177751228 56-57 0.0036316398323566782 58-59 0.0020955415220648196 60-61 0.007974436289848476 62-63 0.010932847850410574 64-65 0.007433957254745786 66-67 0.008145113879880905 68-69 0.014583451859437522 70-71 0.004655705372551251 72-73 0.01307579981415107 74-75 0.008002882554853881 76-77 0.004778972520908005 78-79 0.016005765109707762 80-81 0.006703836452940396 82-83 0.014820504067815897 84-85 0.023667292484496786 86-87 0.01567389201797804 88-89 0.01629022775976181 90-91 0.03061766323415069 92-93 0.024568090876334606 94-95 0.030304754319091236 96-97 0.017816843981718535 98-99 0.017266882858280708 100 0.01917278261364283 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5273100.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.75624678119216 #Duplication Level Percentage of deduplicated Percentage of total 1 74.52385444203452 46.02313546007928 2 16.184667207573053 19.990086042846993 3 4.835305171947959 8.958308983835789 4 1.816093489461738 4.4862047085166195 5 0.8441789680819979 2.6066662340181996 6 0.46518343538083434 1.7236789820340936 7 0.2935721403523956 1.2690939483379726 8 0.18487307544262424 0.9133653816186128 9 0.13162093479660025 0.7315573437783044 >10 0.6634516605314283 7.11672547358728 >50 0.036192605658585446 1.5427627161262265 >100 0.019311932061081914 2.166987407913985 >500 0.001170313473628527 0.4836387787795148 >1k 3.63200679443396E-4 0.479377037845644 >5k 4.035563104926316E-5 0.1870405338721822 >10k+ 1.2106689314778949E-4 1.3213709668092073 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21131 0.4007320172194724 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18874 0.35792987047467334 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13015 0.2468187593635622 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7505 0.1423261459103753 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 2.8446265005404792E-5 0.0 0.0 0.0 14-15 0.0 3.792835334053972E-5 0.0 0.0 0.0 16-17 0.0 3.792835334053972E-5 0.0 0.0 0.0 18-19 0.0 3.792835334053972E-5 0.0 5.6892530010809584E-5 1.896417667026986E-5 20-21 0.0 3.792835334053972E-5 0.0 5.6892530010809584E-5 1.896417667026986E-5 22-23 0.0 3.792835334053972E-5 0.0 1.1378506002161917E-4 1.896417667026986E-5 24-25 0.0 7.585670668107944E-5 0.0 3.034268267243178E-4 1.896417667026986E-5 26-27 0.0 1.1378506002161917E-4 0.0 5.689253001080958E-4 1.896417667026986E-5 28-29 0.0 1.1378506002161917E-4 0.0 0.0017257400769945573 1.896417667026986E-5 30-31 0.0 1.1378506002161917E-4 0.0 0.005613396294399879 2.8446265005404792E-5 32-33 0.0 1.1378506002161917E-4 0.0 0.012573249132388917 3.792835334053972E-5 34-35 0.0 1.517134133621589E-4 0.0 0.022301871764237355 3.792835334053972E-5 36-37 0.0 1.517134133621589E-4 0.0 0.03845935028730728 3.792835334053972E-5 38-39 0.0 1.517134133621589E-4 0.0 0.06380497240712295 3.792835334053972E-5 40-41 0.0 1.517134133621589E-4 0.0 0.09331323130606284 3.792835334053972E-5 42-43 0.0 1.517134133621589E-4 0.0 0.12070698450626766 3.792835334053972E-5 44-45 0.0 1.517134133621589E-4 0.0 0.15354345641083994 3.792835334053972E-5 46-47 0.0 1.8015967836756367E-4 0.0 0.1922777872598661 3.792835334053972E-5 48-49 0.0 1.896417667026986E-4 0.0 0.23419809978949763 3.792835334053972E-5 50-51 0.0 1.9912385503783356E-4 0.0 0.2781949896645237 3.792835334053972E-5 52-53 0.0 2.0860594337296847E-4 0.0 0.320238569342512 5.6892530010809584E-5 54-55 0.0 2.0860594337296847E-4 0.0 0.36997212266029467 7.585670668107944E-5 56-57 0.0 2.0860594337296847E-4 0.0 0.4256793916292124 7.585670668107944E-5 58-59 0.0 2.0860594337296847E-4 0.0 0.4880620507860651 7.585670668107944E-5 60-61 0.0 2.2757012004323833E-4 0.0 0.5524075022282908 7.585670668107944E-5 62-63 0.0 2.2757012004323833E-4 0.0 0.6149703210635111 7.585670668107944E-5 64-65 0.0 2.2757012004323833E-4 0.0 0.6812121901727637 7.585670668107944E-5 66-67 0.0 2.2757012004323833E-4 0.0 0.7559310462536268 7.585670668107944E-5 68-69 0.0 2.2757012004323833E-4 0.0 0.8426352619901007 9.48208833513493E-5 70-71 0.0 2.2757012004323833E-4 0.0 0.9340331114524663 9.48208833513493E-5 72-73 0.0 2.2757012004323833E-4 0.0 1.0214010733723995 1.517134133621589E-4 74-75 0.0 2.2757012004323833E-4 0.0 1.1100775634825815 1.517134133621589E-4 76-77 0.0 2.6549847338377806E-4 0.0 1.208842995581347 1.517134133621589E-4 78-79 0.0 3.034268267243178E-4 0.0 1.3159526654150309 1.517134133621589E-4 80-81 0.0 3.034268267243178E-4 0.0 1.428324894274715 1.7067759003242875E-4 82-83 0.0 3.034268267243178E-4 0.0 1.5472018357323016 2.0860594337296847E-4 84-85 0.0 3.034268267243178E-4 0.0 1.6660882592782233 2.0860594337296847E-4 86-87 0.0 3.034268267243178E-4 0.0 1.7897252090800477 2.0860594337296847E-4 88 0.0 3.034268267243178E-4 0.0 1.8905387722592024 2.0860594337296847E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9765 0.0 49.644787 1 GTACCTG 5600 0.0 43.368256 1 TACCTGG 5555 0.0 42.926056 2 GTATCAA 16475 0.0 37.18232 1 ACCTGGG 6995 0.0 33.65515 3 TCAACGC 20430 0.0 29.23336 4 ATCAACG 20505 0.0 29.103518 3 CAACGCA 20625 0.0 29.002262 5 TATCAAC 21355 0.0 28.168781 2 AACGCAG 21390 0.0 28.07512 6 TATAACG 535 0.0 27.252415 2 GTACATG 17870 0.0 26.600433 1 TACATGG 18590 0.0 24.692638 2 ACGCAGA 24450 0.0 24.56119 7 ACATGGG 18280 0.0 24.188839 3 CGCAGAG 25130 0.0 23.746994 8 CCTGGGG 6350 0.0 23.087795 4 TAACGCA 660 0.0 22.782825 4 CATGGGG 10815 0.0 22.462887 4 GCAGAGT 27790 0.0 21.287786 9 >>END_MODULE