FastQCFastQC Report
Mon 6 Jun 2016
ERR1211571_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211571_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5118392
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152710.2983554209994076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129950.25388833055381455No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90240.17630537090555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80900.044.5841981
TACCTGG56550.040.7613872
GTACCTG59300.040.3107871
GTATCAA135400.035.204691
ACCTGGG66150.033.742223
TCAACGC161950.028.8993634
ATCAACG162600.028.7549383
CAACGCA164250.028.5491145
AACGCAG171600.027.5144656
GTACATG167100.027.2564131
TATCAAC175050.027.0078142
TACATGG168100.026.277462
ACATGGG165150.025.5509853
TATAACG4700.025.0223222
CATGGGG95950.024.7318334
ACGCAGA192800.024.1478757
CGCAGAG196100.023.6703328
ATGGGGG57100.021.7237745
TAACGCA5850.021.6879144
CCTGGGG62550.021.5607814
Produced by FastQC (version 0.11.5)