FastQCFastQC Report
Mon 6 Jun 2016
ERR1211571_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211571_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5118392
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185780.36296555637004746No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158770.3101950768913362No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115080.22483623763088095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94750.045.625981
TACCTGG56650.040.508022
GTACCTG58000.039.990721
GTATCAA159450.036.0709341
ACCTGGG65650.033.9996453
TCAACGC196050.028.8580254
ATCAACG197450.028.653973
CAACGCA199200.028.4252785
AACGCAG206300.027.7203276
TATCAAC206500.027.5085662
TAACGCA6800.026.9502434
TATAACG6850.026.772882
GTACATG174150.024.363581
ACGCAGA234200.024.2775847
ATAACGC7750.023.6471273
CGCAGAG240050.023.6076418
TACATGG176650.023.3457052
CCTGGGG59100.023.2963094
ACATGGG173300.023.0210233
CATGGGG102350.022.2669584
Produced by FastQC (version 0.11.5)