##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1211571_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5118392
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.847841665898198	31.0	31.0	34.0	30.0	34.0
2	32.13081413068792	33.0	31.0	34.0	30.0	34.0
3	32.21580117349355	34.0	31.0	34.0	30.0	34.0
4	35.90643741237482	37.0	35.0	37.0	35.0	37.0
5	35.79090698797591	37.0	35.0	37.0	35.0	37.0
6	35.820956659825974	37.0	35.0	37.0	35.0	37.0
7	35.71930442217009	37.0	35.0	37.0	33.0	37.0
8	35.69946244836269	37.0	35.0	37.0	33.0	37.0
9	37.302637039132605	39.0	37.0	39.0	34.0	39.0
10-11	37.24935848993199	39.0	37.0	39.0	34.0	39.0
12-13	37.15213215400462	39.0	37.0	39.0	33.0	39.0
14-15	38.303695867764716	40.0	38.0	41.0	33.0	41.0
16-17	38.2242079152984	40.0	38.0	41.0	33.0	41.0
18-19	38.21634890410895	40.0	38.0	41.0	33.0	41.0
20-21	38.14463028623052	40.0	37.5	41.0	33.0	41.0
22-23	38.02651789859003	40.0	37.0	41.0	33.0	41.0
24-25	37.90458341213412	40.0	37.0	41.0	33.0	41.0
26-27	37.67679986214421	39.5	37.0	41.0	32.0	41.0
28-29	37.68013127951122	40.0	37.0	41.0	32.0	41.0
30-31	37.54556001181621	39.0	37.0	41.0	32.0	41.0
32-33	37.3422544619482	39.0	36.0	41.0	31.5	41.0
34-35	37.179211752441006	39.0	36.0	41.0	31.0	41.0
36-37	37.06610972743002	39.0	36.0	41.0	31.0	41.0
38-39	36.97842164101538	39.0	36.0	41.0	30.5	41.0
40-41	36.81299116988304	39.0	35.0	40.5	30.0	41.0
42-43	36.60487356185302	39.0	35.0	40.0	30.0	41.0
44-45	36.385852920214006	39.0	35.0	40.0	30.0	41.0
46-47	36.111573810681165	38.5	35.0	40.0	28.5	41.0
48-49	35.84308382398222	38.0	35.0	40.0	27.5	41.0
50-51	35.624471220648985	38.0	34.0	40.0	27.0	41.0
52-53	35.40147100886372	38.0	34.0	40.0	26.0	41.0
54-55	35.17367475957293	38.0	34.0	40.0	26.0	41.0
56-57	34.95175662590908	38.0	33.5	40.0	25.5	41.0
58-59	34.668798423411104	38.0	33.0	40.0	25.0	41.0
60-61	34.338519890621896	37.0	33.0	40.0	24.5	41.0
62-63	33.98235676360857	36.5	32.0	39.5	23.0	41.0
64-65	33.64373488783196	36.0	32.0	39.0	23.0	40.0
66-67	33.27354450382073	36.0	31.0	39.0	22.0	40.0
68-69	32.86612865915701	35.0	31.0	38.5	22.0	40.0
70-71	32.487719287620024	35.0	30.5	38.0	21.0	40.0
72-73	32.05060163426326	35.0	30.0	37.0	20.0	39.5
74-75	31.642356036817812	34.0	30.0	37.0	20.0	39.0
76-77	30.234423819043165	32.5	28.5	35.0	19.5	37.5
78-79	31.128887060623725	34.0	29.5	36.0	20.0	38.0
80-81	31.092974609994698	34.0	29.5	35.5	20.0	37.5
82-83	30.79246685287098	34.0	29.0	35.0	19.0	37.0
84-85	30.445279396341665	34.0	29.0	35.0	18.0	36.5
86-87	30.12814688675662	34.0	29.0	35.0	18.0	36.0
88-89	29.819423561149673	34.0	29.0	35.0	17.5	36.0
90-91	29.50570569819584	33.0	27.5	35.0	16.5	35.0
92-93	29.242224901883247	33.0	27.0	35.0	15.0	35.0
94-95	28.99637151667946	33.0	27.0	35.0	13.5	35.0
96-97	28.733112372010584	33.0	26.0	34.5	11.0	35.0
98-99	28.495377161421008	32.0	25.5	34.0	10.5	35.0
100	26.67278981367586	30.0	23.0	34.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.1332213377024054
1101	2	0.09910357069339426
1101	3	0.03532958953363163
1101	4	0.04646321817113375
1101	5	0.01971866272629086
1101	6	0.028215098370786507
1101	7	0.03724240833651038
1101	8	0.037729505204993075
1101	9	0.006394211290732699
1101	10-11	0.03683151908219173
1101	12-13	0.049382490199811
1101	14-15	0.021876827490189044
1101	16-17	0.003740808258875461
1101	18-19	0.05959267283801495
1101	20-21	-0.027830423879997568
1101	22-23	-0.061109290901079305
1101	24-25	-0.10708591071516338
1101	26-27	-0.12026001933683261
1101	28-29	-0.034008834862774506
1101	30-31	7.597277380000378E-4
1101	32-33	-0.028354171767652758
1101	34-35	-0.14961057515336051
1101	36-37	-0.2195491325115313
1101	38-39	-0.29475508767622927
1101	40-41	-0.34400574072214596
1101	42-43	-0.3279005660074148
1101	44-45	-0.3006584524258784
1101	46-47	-0.44482643769726593
1101	48-49	-0.3907855858841174
1101	50-51	-0.4924758506402753
1101	52-53	-0.4673322559722166
1101	54-55	-0.42286004704762803
1101	56-57	-0.5200975163411599
1101	58-59	-0.6243883530510814
1101	60-61	-0.7296530581332874
1101	62-63	-0.895385241170942
1101	64-65	-0.8290702519643887
1101	66-67	-0.8710861738831994
1101	68-69	-0.8393961676552166
1101	70-71	-0.9742943054022888
1101	72-73	-1.1053872351177247
1101	74-75	-0.9589896513255773
1101	76-77	-0.8783913515308868
1101	78-79	-0.7625695694114079
1101	80-81	-0.6954138016972706
1101	82-83	-0.8534525814395799
1101	84-85	-0.9443645078102847
1101	86-87	-1.103474953711931
1101	88-89	-0.9755411335615669
1101	90-91	-1.1472898221869805
1101	92-93	-1.2943254630452685
1101	94-95	-1.412257156493915
1101	96-97	-1.4541377297891422
1101	98-99	-1.467627266477944
1101	100	-1.6273508258358618
1102	1	0.18634729236906722
1102	2	0.0958235020628706
1102	3	0.07899611113654004
1102	4	0.04241011858577792
1102	5	0.07272585783652374
1102	6	0.07355214502376128
1102	7	0.09172312045631514
1102	8	0.026942230916510823
1102	9	0.04964487911790627
1102	10-11	0.08839555118789377
1102	12-13	0.13248476327334657
1102	14-15	0.06559147796597387
1102	16-17	0.1069036408930657
1102	18-19	-0.04639042580195252
1102	20-21	-0.05703558648292528
1102	22-23	-0.06079799632035332
1102	24-25	-0.0014164896601940313
1102	26-27	-0.020419075104598505
1102	28-29	0.05380405164293478
1102	30-31	0.017205836292049526
1102	32-33	0.06632375934712087
1102	34-35	0.0954914330551091
1102	36-37	-0.02610587106158846
1102	38-39	-0.05856030012691349
1102	40-41	-0.10543898495976833
1102	42-43	-0.17242938356070425
1102	44-45	-0.0868743158898937
1102	46-47	-0.13789449317360436
1102	48-49	-0.21319205687357368
1102	50-51	-0.15178384573675885
1102	52-53	-0.18662123000684971
1102	54-55	-0.2135498252094692
1102	56-57	-0.25781174256405137
1102	58-59	-0.2290693547254392
1102	60-61	-0.2731050116889193
1102	62-63	-0.3396201092892355
1102	64-65	-0.404222627507103
1102	66-67	-0.5289633311856932
1102	68-69	-0.6594420190010695
1102	70-71	-0.534410627262563
1102	72-73	-0.5855013966479099
1102	74-75	-0.5475468164724617
1102	76-77	-0.5003179987532818
1102	78-79	-0.6400479524375413
1102	80-81	-0.42595440379259486
1102	82-83	-0.6814541120314921
1102	84-85	-0.6848434486938082
1102	86-87	-0.6843771083384382
1102	88-89	-0.7438066268004739
1102	90-91	-0.6720173002574796
1102	92-93	-0.8906711260165849
1102	94-95	-0.9063021851173119
1102	96-97	-0.9661503956520967
1102	98-99	-0.9776051396974275
1102	100	-0.924206940038804
1103	1	0.2076715535463869
1103	2	0.14106507970955562
1103	3	0.11650534057745432
1103	4	0.13797801003131127
1103	5	0.07701722220611629
1103	6	0.12387930169392547
1103	7	0.09630052470076578
1103	8	0.1274419156826383
1103	9	0.1790596475106625
1103	10-11	0.0697533065388285
1103	12-13	0.12491616197691258
1103	14-15	0.1571863805254523
1103	16-17	0.17879826542760924
1103	18-19	0.14959891662662272
1103	20-21	0.1508665160059337
1103	22-23	0.0057406790598690804
1103	24-25	0.0022158642771117343
1103	26-27	0.049808848026764
1103	28-29	0.1078391524825264
1103	30-31	0.24534202253534687
1103	32-33	0.2612593593810786
1103	34-35	0.13431397094370823
1103	36-37	0.17752793108188314
1103	38-39	0.1434902065613457
1103	40-41	0.11076344606500044
1103	42-43	0.05738653027884055
1103	44-45	0.02183336469068564
1103	46-47	0.048788592115471374
1103	48-49	0.06126542517249334
1103	50-51	0.09167597090961266
1103	52-53	-0.02044018492610178
1103	54-55	0.045981861694798454
1103	56-57	-0.07969966244039739
1103	58-59	-0.15719534222581188
1103	60-61	-0.07361871110629892
1103	62-63	-0.034177911411866546
1103	64-65	-0.14041754471721646
1103	66-67	-0.14770980070424855
1103	68-69	-0.10882326716782131
1103	70-71	-0.2196712905808269
1103	72-73	-0.23067697747053728
1103	74-75	-0.30299574965789944
1103	76-77	-0.2161581100490757
1103	78-79	-0.03868164270899754
1103	80-81	-0.06695953396717158
1103	82-83	-0.032367531676893435
1103	84-85	-0.22838698544927993
1103	86-87	-0.2960381333864248
1103	88-89	-0.20295388009740378
1103	90-91	-0.2667425119484399
1103	92-93	-0.34352055977563367
1103	94-95	-0.38294384557993766
1103	96-97	-0.49024820152013504
1103	98-99	-0.5057942812467822
1103	100	-0.47687513347746346
1104	1	0.3188626938262935
1104	2	0.15236911375388473
1104	3	0.1670923549083554
1104	4	0.09706675139049992
1104	5	0.1670093886995616
1104	6	0.12315390145825234
1104	7	0.1707922760969396
1104	8	0.18115288830374965
1104	9	0.2284032588962006
1104	10-11	0.2084379544072945
1104	12-13	0.1811747619854387
1104	14-15	0.1651720541541195
1104	16-17	0.22843468887578666
1104	18-19	0.24842364886848145
1104	20-21	0.1265470216660347
1104	22-23	0.1661294213559401
1104	24-25	0.07401288337400302
1104	26-27	0.11084008443636861
1104	28-29	0.14739197621684497
1104	30-31	0.2592620231518694
1104	32-33	0.11256330427091399
1104	34-35	0.22801537985667863
1104	36-37	0.28659322783185104
1104	38-39	0.27856757301622537
1104	40-41	0.1692072377843985
1104	42-43	0.008443358560946024
1104	44-45	0.05617291728020035
1104	46-47	0.22732284240024114
1104	48-49	0.1467678318904504
1104	50-51	-0.04205368939968679
1104	52-53	-0.021157727477728372
1104	54-55	-0.04554606647872106
1104	56-57	0.024662168194481637
1104	58-59	-0.05518617019300365
1104	60-61	-0.07219069036322168
1104	62-63	0.04393729009391478
1104	64-65	0.03977667909924065
1104	66-67	0.056322180976465575
1104	68-69	-0.1465168041924656
1104	70-71	-0.08490804260109286
1104	72-73	0.05668687682020135
1104	74-75	-0.042105339568390576
1104	76-77	0.10635048278175319
1104	78-79	0.08703061101683218
1104	80-81	0.19367606805079873
1104	82-83	0.12918807669976218
1104	84-85	-0.09918705332758293
1104	86-87	-0.1610306172094198
1104	88-89	-0.06920563334315588
1104	90-91	-0.02166425545973283
1104	92-93	-0.1193673536743205
1104	94-95	-0.15735585659398765
1104	96-97	-0.3505324970621366
1104	98-99	-0.32950219088220933
1104	100	-0.0777007040737665
1105	1	0.3415093202998314
1105	2	0.20744820701960265
1105	3	0.12885842427579774
1105	4	0.10980291952991905
1105	5	0.08721985862330683
1105	6	0.09863696996475113
1105	7	0.06793525656961208
1105	8	0.13265992367811918
1105	9	0.1291651879298641
1105	10-11	0.15836383403604515
1105	12-13	0.2110953553268402
1105	14-15	0.16044174350491147
1105	16-17	0.151372194197549
1105	18-19	0.20042863891087137
1105	20-21	0.11606876048795556
1105	22-23	0.16041377098886045
1105	24-25	0.16662602115806635
1105	26-27	0.1996770598285238
1105	28-29	0.21999024853514726
1105	30-31	0.26720276292461875
1105	32-33	0.29255126229755035
1105	34-35	0.29282620882221977
1105	36-37	0.33468595132235635
1105	38-39	0.2449255132090471
1105	40-41	0.17936382778609783
1105	42-43	0.24052977753292026
1105	44-45	0.14914800905109615
1105	46-47	0.2222795823574728
1105	48-49	0.282431467910051
1105	50-51	0.16915869458582478
1105	52-53	0.15124634473744436
1105	54-55	0.07525143855922067
1105	56-57	0.16858725428882337
1105	58-59	0.20366385239493923
1105	60-61	0.08192424676898469
1105	62-63	0.09949955534490584
1105	64-65	0.1314806818949421
1105	66-67	0.0725622884051802
1105	68-69	0.06463105338288244
1105	70-71	0.016545804680795584
1105	72-73	0.047904263265543534
1105	74-75	0.0821830959980332
1105	76-77	0.1586027575409723
1105	78-79	0.1706418341478262
1105	80-81	0.11485376890606602
1105	82-83	0.07565458682767101
1105	84-85	0.11250177652255644
1105	86-87	0.17123301656321743
1105	88-89	0.200282672963489
1105	90-91	0.2689340913632883
1105	92-93	0.2129136497505293
1105	94-95	0.1179743441903156
1105	96-97	0.07683064781761217
1105	98-99	0.2460130743072959
1105	100	0.27255618938684734
1106	1	0.2188626138174179
1106	2	0.13493205128425956
1106	3	0.0979630100807043
1106	4	0.05875642691376015
1106	5	0.11304438138311212
1106	6	0.08307596262664418
1106	7	0.06703006946950296
1106	8	0.1103001158433301
1106	9	0.07353927180934505
1106	10-11	0.08826199887943176
1106	12-13	0.10651273040287634
1106	14-15	0.17483509021179344
1106	16-17	0.10040689830062632
1106	18-19	0.05481194330756267
1106	20-21	0.07692833307557834
1106	22-23	0.07252388738904614
1106	24-25	0.0296428681999501
1106	26-27	0.08391240504446529
1106	28-29	0.06858048411961448
1106	30-31	0.16446082921573435
1106	32-33	0.09883845378931966
1106	34-35	0.11749560508022228
1106	36-37	0.1403690177849768
1106	38-39	0.07589497095144537
1106	40-41	0.18785123420173733
1106	42-43	0.24615246722274975
1106	44-45	0.13735670902320152
1106	46-47	0.16074158036344954
1106	48-49	0.12028671114934042
1106	50-51	0.16296864617006435
1106	52-53	0.14097561182960305
1106	54-55	0.026581374719484074
1106	56-57	0.11239612325014292
1106	58-59	0.09447009347669422
1106	60-61	0.10986331432122398
1106	62-63	0.11750316365922231
1106	64-65	0.09017245393214068
1106	66-67	0.11626656098547983
1106	68-69	0.09783827841524584
1106	70-71	0.1109674596546526
1106	72-73	-0.007440367410687543
1106	74-75	0.1073782503922125
1106	76-77	0.10742751140325879
1106	78-79	0.24323629246191203
1106	80-81	0.19218673912600792
1106	82-83	0.22556900959456883
1106	84-85	0.14564191823682293
1106	86-87	0.12643985042211625
1106	88-89	0.17288852561586765
1106	90-91	0.06794523501899263
1106	92-93	0.016826270432616752
1106	94-95	0.05819624758862574
1106	96-97	0.06744114495805675
1106	98-99	0.029589534497642234
1106	100	-0.009949403093017395
1107	1	0.2826245375174814
1107	2	0.20817002765151216
1107	3	0.13770029288244956
1107	4	0.11335804532467364
1107	5	0.12564718396239272
1107	6	0.14564050989584842
1107	7	0.20768504181783953
1107	8	0.112211510699467
1107	9	0.16937915680880167
1107	10-11	0.14475477284279492
1107	12-13	0.21123597866266408
1107	14-15	0.24919286791275397
1107	16-17	0.2519257841698561
1107	18-19	0.18859267264947022
1107	20-21	0.14960614176670362
1107	22-23	0.2659005013845146
1107	24-25	0.15025306202341682
1107	26-27	0.19245990266727375
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2316	86-87	0.3150921612887174
2316	88-89	0.4209357368262836
2316	90-91	0.3746721682615082
2316	92-93	0.33613770818369204
2316	94-95	0.3752416592166057
2316	96-97	0.3767837176055977
2316	98-99	0.48819882368245615
2316	100	0.19181589054171866
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	8.0
11	26.0
12	92.0
13	236.0
14	750.0
15	1980.0
16	4625.0
17	9279.0
18	16237.0
19	24019.0
20	32414.0
21	42530.0
22	53746.0
23	60477.0
24	64632.0
25	71767.0
26	83272.0
27	99385.0
28	116983.0
29	139481.0
30	164804.0
31	197816.0
32	238132.0
33	294602.0
34	378437.0
35	505140.0
36	706072.0
37	962289.0
38	763470.0
39	85684.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.11051235846097	20.47064334338188	13.888517905071573	23.530326393085577
2	14.994161006078302	21.868356836921286	39.97172100851048	23.165761148489928
3	17.08301340476288	27.81483413535274	31.067985065441267	24.03416739444311
4	11.7187362626413	17.92029528312098	38.79260119780976	31.56836725642796
5	12.081057868445567	38.39391774878243	35.673323997024056	13.851700385747955
6	29.94032472850721	38.46782802643342	18.495543892115613	13.09630335294375
7	26.327526301228982	33.051376291616585	23.249157157169673	17.37194024998476
8	25.674425801732355	36.28510259352909	20.59945443398405	17.441017170754506
9	26.455261730637282	15.288219425163216	20.683800693655353	37.572718150544155
10-11	24.450401846210596	26.593650017114808	29.3845157135779	19.571432423096702
12-13	25.015832836506757	24.422444597771708	29.307496202121847	21.254226363599685
14-15	22.29533656182187	25.781197645900832	27.02321882013312	24.900246972144178
16-17	21.763418827064072	28.5232535171725	27.662806661551777	22.050520994211652
18-19	21.548200128283852	27.577414304685295	29.661460353235825	21.212925213795028
20-21	22.431405096409893	26.836046181873634	29.651398889305362	21.081149832411107
22-23	22.094843867926464	26.828118445816273	29.44180114414862	21.635236542108643
24-25	21.79457159539281	26.973122317821918	29.653964531630688	21.578341555154584
26-27	21.902984928825138	27.03099418175282	29.485774795931523	21.58024609349052
28-29	21.8384438012644	27.069771749234807	29.64937895613364	21.442405493367154
30-31	22.165777550748672	26.972483857634778	29.611277133470352	21.250461458146198
32-33	21.958159322812108	27.0864411001861	29.519179012255226	21.436220564746566
34-35	22.173008851373016	27.194169656493735	29.44701729459385	21.185804197539404
36-37	22.156730731470386	27.17202192052958	29.287027917629793	21.384219430370237
38-39	22.079505923428684	27.35520154897147	29.20665033942365	21.358642188176198
40-41	22.20292782469003	27.183441948579794	29.264384242016416	21.349245984713757
42-43	22.002307656985646	27.242721159416895	29.18474836085034	21.57022282274713
44-45	22.054050832753674	27.404786282034248	29.009810549515823	21.531352335696255
46-47	22.018528863131777	27.378206507180735	28.75305119958328	21.85021343010421
48-49	22.00871168829088	27.40770367090775	28.792582120611364	21.79100252019
50-51	22.049726545713018	27.65211288533716	28.514673763266927	21.783486805682895
52-53	22.062614773855817	27.73030139206681	28.334642708583868	21.87244112549351
54-55	21.991637521736582	27.69739747171802	28.470174478810495	21.840790527734903
56-57	22.147238412759446	27.710280300792306	28.266411577784133	21.87606970866411
58-59	22.110929431189884	27.670676932352873	28.45180833174263	21.76658530471461
60-61	22.251428666332224	27.827171795080965	28.352213693996987	21.569185844589825
62-63	22.10445042003934	27.78543506711993	28.4337732182278	21.67634129461294
64-65	22.04646154513477	27.57209353035411	28.412278609053192	21.969166315457933
66-67	21.956454733604826	27.670240811487673	28.327000302363764	22.04630415254374
68-69	21.949844792771092	27.689854805132175	28.369538583348607	21.990761818748126
70-71	21.947126755230304	27.515116145256453	28.381592133020124	22.156164966493122
72-73	21.90886768450199	27.776546226847643	28.14355274620823	22.171033342442144
74-75	22.002184442781473	27.81713986490843	27.998440800232906	22.182234892077194
76-77	21.821077575852428	27.75985950379879	28.245191544476413	22.173871375872377
78-79	21.82212278276332	27.696535956971807	28.19822865321954	22.28311260704533
80-81	21.840410664819608	27.89036042588548	27.96806591582622	22.301162993468697
82-83	21.96542764938049	27.9346679903225	28.01602377812683	22.083880582170178
84-85	21.90161994979116	27.94461022977974	27.919479428944566	22.234290391484528
86-87	21.790110568549345	27.898966985367835	28.08021424909691	22.23070819698591
88-89	21.734245346709354	27.815290911879853	28.10681342773929	22.343650313671507
90-91	21.957367488434876	27.902928593582082	27.96993214436964	22.1697717736134
92-93	21.850911523345857	27.899743277724177	27.98556164373389	22.26378355519607
94-95	21.69736754034238	27.792014141146666	28.029790994985817	22.480827323525144
96-97	21.69654875530297	27.84057662186869	28.04439563183844	22.418478990989893
98-99	21.6803444784148	27.766029497801085	27.926643965628756	22.626982058155352
100	20.70546656567859	28.296045128849684	28.20943925825355	22.789049047218178
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1004.0
1	820.0
2	1632.5
3	2446.0
4	1731.0
5	1366.5
6	2033.0
7	3878.5
8	6156.0
9	7198.0
10	6445.5
11	5485.0
12	5572.5
13	5969.5
14	6414.0
15	6871.0
16	7355.0
17	7933.0
18	8518.5
19	9137.0
20	10102.0
21	11562.5
22	13623.0
23	16527.5
24	20521.5
25	25682.5
26	31627.0
27	38590.5
28	46823.5
29	56220.5
30	67180.0
31	77602.0
32	88828.5
33	101634.5
34	114051.0
35	125710.5
36	139127.5
37	151640.0
38	160567.0
39	169006.5
40	171616.0
41	171978.0
42	174846.0
43	178214.5
44	181957.5
45	185607.5
46	187636.0
47	188464.0
48	189662.5
49	188952.5
50	186665.5
51	179618.0
52	169211.5
53	160490.5
54	151786.0
55	140064.5
56	126787.5
57	113843.0
58	99947.5
59	85664.0
60	70830.5
61	57027.5
62	45828.5
63	35483.0
64	26576.0
65	20050.0
66	15126.5
67	11708.0
68	8961.0
69	6887.5
70	5367.0
71	4069.0
72	3253.0
73	2673.0
74	2133.5
75	1606.5
76	1135.5
77	770.5
78	532.5
79	393.5
80	279.0
81	170.5
82	122.5
83	92.5
84	60.5
85	45.5
86	34.0
87	23.5
88	16.0
89	7.5
90	5.5
91	3.5
92	3.5
93	3.0
94	2.0
95	1.5
96	0.5
97	1.0
98	1.5
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.23151802362929608
2	0.07113562228137274
3	0.0012113179295372453
4	9.768692980139076E-4
5	4.688972630466756E-4
6	4.688972630466756E-4
7	0.0
8	7.424206664905697E-4
9	0.0
10-11	3.125981753644504E-4
12-13	0.001435997868080444
14-15	1.953738596027815E-5
16-17	9.768692980139076E-5
18-19	0.0010745562278152983
20-21	0.0012406240084776624
22-23	1.953738596027815E-5
24-25	9.768692980139076E-6
26-27	2.344486315233378E-4
28-29	0.0014457665610605832
30-31	0.002061194218809345
32-33	0.0032627434553664505
34-35	0.003966089349936464
36-37	0.0016606778066236426
38-39	0.0030673695957636696
40-41	0.006369187823050677
42-43	0.012640688716299963
44-45	0.008440150734840161
46-47	0.0063105756651698425
48-49	0.006359419130070538
50-51	0.005089489042652458
52-53	0.003419042543048676
54-55	0.00570491670040122
56-57	0.003438579929008954
58-59	0.0021686498415908746
60-61	0.008195933410336684
62-63	0.010511113646629645
64-65	0.006896697243978188
66-67	0.007512124901726948
68-69	0.014027843119479712
70-71	0.0036925659464925706
72-73	0.012416008777756763
74-75	0.0074632814368262535
76-77	0.004815965639208564
78-79	0.01596204432954725
80-81	0.0068283163931172125
82-83	0.014604196005307919
84-85	0.022770823336704184
86-87	0.015581065303321826
88-89	0.015180548891136122
90-91	0.029286541554456945
92-93	0.02322995190677072
94-95	0.02753794551101205
96-97	0.016831458004779625
98-99	0.016763077153918653
100	0.01867774097802591
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5118392.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.947756564484955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.45446189711531	46.867413426393355
2	16.309925203274105	20.533464025613128
3	4.895687607851105	9.245176552643313
4	1.8057834667755748	4.546800722990425
5	0.8487548541483958	2.6713606970929082
6	0.46051755286400703	1.7393128086813512
7	0.27369121576836686	1.2059773616817562
8	0.17274645971315472	0.8699201674720217
9	0.13068768334561334	0.7403846829494547
>10	0.6006882574949609	6.417725135590596
>50	0.030019622855807976	1.3009548627244185
>100	0.015401453053531626	1.792671270749073
>500	0.0012758835670381946	0.5393838324286158
>1k	2.3922811529336147E-4	0.3758694741950752
>5k	0.0	0.0
>10k+	1.19614057646677E-4	1.153584978794605
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18578	0.36296555637004746	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15877	0.3101950768913362	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11508	0.22483623763088095	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	9.768692980139076E-6	0.0
12-13	0.0	0.0	0.0	1.953738596027815E-5	0.0
14-15	0.0	0.0	0.0	1.953738596027815E-5	9.768692980139076E-6
16-17	0.0	0.0	0.0	1.953738596027815E-5	1.953738596027815E-5
18-19	0.0	0.0	0.0	2.9306078940417225E-5	2.9306078940417225E-5
20-21	0.0	0.0	0.0	5.861215788083445E-5	3.90747719205563E-5
22-23	0.0	0.0	0.0	7.81495438411126E-5	5.861215788083445E-5
24-25	1.953738596027815E-5	0.0	0.0	7.81495438411126E-5	7.81495438411126E-5
26-27	1.953738596027815E-5	0.0	0.0	1.5629908768222518E-4	7.81495438411126E-5
28-29	1.953738596027815E-5	0.0	0.0	6.740398156295962E-4	7.81495438411126E-5
30-31	1.953738596027815E-5	1.953738596027815E-5	0.0	0.0026570844905978285	7.81495438411126E-5
32-33	1.953738596027815E-5	1.953738596027815E-5	0.0	0.0076000431385482	9.768692980139075E-5
34-35	1.953738596027815E-5	1.953738596027815E-5	0.0	0.014750726400010003	9.768692980139075E-5
36-37	3.90747719205563E-5	1.953738596027815E-5	0.0	0.0259749546341898	9.768692980139075E-5
38-39	3.90747719205563E-5	2.9306078940417225E-5	0.0	0.04561002752426934	9.768692980139075E-5
40-41	3.90747719205563E-5	3.90747719205563E-5	0.0	0.06887905420296062	1.172243157616689E-4
42-43	5.861215788083445E-5	3.90747719205563E-5	0.0	0.09072185170655159	1.172243157616689E-4
44-45	5.861215788083445E-5	3.90747719205563E-5	0.0	0.11670657503372153	1.172243157616689E-4
46-47	5.861215788083445E-5	3.90747719205563E-5	0.0	0.14681368679851015	1.172243157616689E-4
48-49	7.814954384111259E-5	3.90747719205563E-5	0.0	0.18033007241336732	1.3676170172194704E-4
50-51	9.768692980139075E-5	3.90747719205563E-5	0.0	0.21731434403617386	1.3676170172194704E-4
52-53	9.768692980139075E-5	4.884346490069538E-5	0.0	0.2529505360277212	1.5629908768222518E-4
54-55	9.768692980139075E-5	8.791823682125168E-5	0.0	0.29585463559649205	1.7583647364250335E-4
56-57	9.768692980139075E-5	9.768692980139075E-5	0.0	0.3458312688828835	1.953738596027815E-4
58-59	9.768692980139075E-5	9.768692980139075E-5	0.0	0.40124906415921247	2.1491124556305964E-4
60-61	9.768692980139075E-5	1.172243157616689E-4	0.0	0.45653986642679967	2.1491124556305964E-4
62-63	1.0745562278152983E-4	1.172243157616689E-4	9.768692980139076E-6	0.5140090872289579	2.344486315233378E-4
64-65	1.172243157616689E-4	1.172243157616689E-4	1.953738596027815E-5	0.5708921864523077	2.344486315233378E-4
66-67	1.172243157616689E-4	1.172243157616689E-4	1.953738596027815E-5	0.6365768780507628	2.344486315233378E-4
68-69	1.2699300874180797E-4	1.172243157616689E-4	1.953738596027815E-5	0.709070348656375	2.344486315233378E-4
70-71	1.3676170172194704E-4	1.172243157616689E-4	1.953738596027815E-5	0.7914301991719275	2.5398601748361595E-4
72-73	1.3676170172194704E-4	1.172243157616689E-4	1.953738596027815E-5	0.8728131803894661	3.516729472850067E-4
74-75	1.3676170172194704E-4	1.172243157616689E-4	1.953738596027815E-5	0.9524377968705797	3.516729472850067E-4
76-77	1.3676170172194704E-4	1.172243157616689E-4	1.953738596027815E-5	1.0373472762539486	3.809790262254239E-4
78-79	1.3676170172194704E-4	1.2699300874180797E-4	1.953738596027815E-5	1.1386290850720304	3.90747719205563E-4
80-81	1.3676170172194704E-4	1.3676170172194704E-4	1.953738596027815E-5	1.242821964398194	4.2005379814598023E-4
82-83	1.3676170172194704E-4	1.3676170172194704E-4	1.953738596027815E-5	1.3494277108904515	4.688972630466756E-4
84-85	1.3676170172194704E-4	1.3676170172194704E-4	1.953738596027815E-5	1.4557013218213846	4.7866595602681465E-4
86-87	1.3676170172194704E-4	1.3676170172194704E-4	1.953738596027815E-5	1.5677189242246392	4.884346490069537E-4
88	1.3676170172194704E-4	1.3676170172194704E-4	1.953738596027815E-5	1.6563600443264213	4.884346490069537E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9475	0.0	45.62598	1
TACCTGG	5665	0.0	40.50802	2
GTACCTG	5800	0.0	39.99072	1
GTATCAA	15945	0.0	36.070934	1
ACCTGGG	6565	0.0	33.999645	3
TCAACGC	19605	0.0	28.858025	4
ATCAACG	19745	0.0	28.65397	3
CAACGCA	19920	0.0	28.425278	5
AACGCAG	20630	0.0	27.720327	6
TATCAAC	20650	0.0	27.508566	2
TAACGCA	680	0.0	26.950243	4
TATAACG	685	0.0	26.77288	2
GTACATG	17415	0.0	24.36358	1
ACGCAGA	23420	0.0	24.277584	7
ATAACGC	775	0.0	23.647127	3
CGCAGAG	24005	0.0	23.607641	8
TACATGG	17665	0.0	23.345705	2
CCTGGGG	5910	0.0	23.296309	4
ACATGGG	17330	0.0	23.021023	3
CATGGGG	10235	0.0	22.266958	4
>>END_MODULE