FastQCFastQC Report
Mon 6 Jun 2016
ERR1211570_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211570_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4641404
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134070.2888565615059581No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120900.26048152671045227No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78640.16943149098850263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74650.044.979411
GTACCTG41300.036.882131
GTATCAA121650.034.5793081
TACCTGG44750.033.9597362
TCAACGC141500.028.6583964
ATCAACG142150.028.296573
CAACGCA144500.028.0935085
GTACATG143350.027.4038031
AACGCAG150200.027.2115486
ACCTGGG54400.026.8638553
TATCAAC152300.026.8148042
TACATGG149350.025.4857922
ACATGGG151450.024.666343
ACGCAGA172250.023.428657
CGCAGAG176300.022.7312878
CATGGGG89100.022.7298774
TAACGCA4750.020.7741494
GCAGAGT200200.019.4778639
GAGTACT125300.019.36796612-13
ATGGGGG54800.018.8626275
Produced by FastQC (version 0.11.5)