FastQCFastQC Report
Mon 6 Jun 2016
ERR1211570_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211570_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4641404
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165470.3565085047541649No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148230.31936457158221954No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103520.22303596067052125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82350.046.7078671
GTACCTG45400.037.585141
TACCTGG45400.036.8788872
GTATCAA138300.036.2986831
ACCTGGG56350.029.2712443
TCAACGC170400.028.6253684
ATCAACG171450.028.368143
CAACGCA173650.028.089625
TATAACG4950.027.5534822
AACGCAG180050.027.2216516
TATCAAC179850.027.1176472
ACGCAGA202900.023.9939087
GTACATG156300.023.764611
CGCAGAG209050.023.2655568
TAACGCA5150.022.8116174
TACATGG161150.022.7055992
ACATGGG158750.022.0827453
CCTGGGG55000.020.505574
GTACCGT5300.020.3927256
GCAGAGT235950.020.2745449
Produced by FastQC (version 0.11.5)