FastQCFastQC Report
Mon 6 Jun 2016
ERR1211569_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211569_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5759809
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135960.23604949400231845No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113270.19665582660813927No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75690.13141060753924305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75250.047.709521
GTATCAA135600.038.722411
TCAACGC158150.032.265194
ATCAACG159550.032.0112463
CAACGCA160100.031.8107435
AACGCAG167500.030.4583026
TATCAAC171750.029.997912
GTACATG174550.028.6762521
TACATGG176950.027.3614062
GTACCTG51100.027.1427141
ACGCAGA187400.026.9481857
TACCTGG51300.026.0480122
ACATGGG178550.025.9469833
CGCAGAG194050.025.8079228
CATGGGG112250.023.8595434
GCAGAGT219350.022.3814479
TAACGCA5750.020.4289384
AGAGTAC199250.019.77188910-11
TATAACG5800.019.4695912
GAGTACT136600.019.3993412-13
Produced by FastQC (version 0.11.5)