FastQCFastQC Report
Mon 6 Jun 2016
ERR1211569_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211569_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5759809
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166990.2899228082042304No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140850.2445393588572121No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99460.1726793371099632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86750.047.8876341
GTATCAA147250.037.2427181
TCAACGC178300.030.2547154
TACCTGG48100.029.7261312
CAACGCA181350.029.7202285
ATCAACG180600.029.6615183
AACGCAG189950.028.5485536
GTACCTG51600.028.328851
TATCAAC191950.028.1296042
GTACATG187800.027.8955821
TACATGG191900.026.7153822
ACATGGG192200.025.6465173
ACGCAGA218600.024.6564677
CGCAGAG224550.024.0031348
CATGGGG121850.023.7551444
ACCTGGG65450.021.2514383
GCAGAGT253200.020.9528969
AGAGTAC232950.019.65771110-11
TATAACG5550.019.4914782
TAACGCA5550.019.4732044
Produced by FastQC (version 0.11.5)