FastQCFastQC Report
Mon 6 Jun 2016
ERR1211568_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211568_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2887985
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100990.34969018190883955No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85140.2948076253858659No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56150.19442621758769524No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35250.1220574206583483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48600.050.5644651
TACCTGG29050.041.460732
TAACGCA2450.040.2809374
GTACCTG31300.038.879381
GTATCAA83500.037.5646361
TATAACG2500.035.756642
ACCTGGG36600.032.742593
TCAACGC101350.030.1394984
CAACGCA101800.030.0031495
ATCAACG102850.029.7004473
AACGCAG105350.029.03126
TATCAAC107950.028.5470872
GTACATG97550.027.1739651
TACATGG101100.026.0603372
ACGCAGA120250.025.3172197
ACATGGG102000.025.1102053
CGCAGAG122250.024.8274578
CATGGGG63500.023.6082254
GTATACG2154.7690264E-821.938431
ATAACGC4350.021.607013
Produced by FastQC (version 0.11.5)