FastQCFastQC Report
Mon 6 Jun 2016
ERR1211568_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211568_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2887985
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116810.4044688597759337No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102070.3534298135204996No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72770.251974992944908No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38020.13164888321788376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55750.049.3925251
TACCTGG32050.040.3459362
GTACCTG33100.039.6013571
GTATCAA95550.037.8493081
TATAACG2950.035.0667722
ACCTGGG38950.032.331183
TCAACGC118750.030.1509194
CAACGCA120200.029.8252625
ATCAACG120500.029.7530673
TATCAAC123700.029.1555212
AACGCAG125400.028.8147956
GTACATG106850.025.6827621
ACGCAGA141150.025.4330967
TAACGCA3700.025.3984644
CGCAGAG146050.024.6119828
TACATGG111100.024.5899242
ACATGGG109350.024.1926733
CCTGGGG35350.023.5268124
GTATAAC7900.022.6803511
CATGGGG68050.022.1644084
Produced by FastQC (version 0.11.5)