##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211568_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2887985 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.021181204196004 33.0 31.0 34.0 30.0 34.0 2 32.27781169223525 34.0 31.0 34.0 30.0 34.0 3 32.369605797814046 34.0 31.0 34.0 30.0 34.0 4 36.00768979063257 37.0 35.0 37.0 35.0 37.0 5 35.90651994383627 37.0 35.0 37.0 35.0 37.0 6 35.937469550569 37.0 35.0 37.0 35.0 37.0 7 35.84525646774481 37.0 35.0 37.0 35.0 37.0 8 35.827770573600624 37.0 35.0 37.0 35.0 37.0 9 37.45694385531781 39.0 37.0 39.0 34.0 39.0 10-11 37.41796356283014 39.0 37.0 39.0 34.0 39.0 12-13 37.3237476995206 39.0 37.0 39.0 34.0 39.0 14-15 38.537264909616916 40.0 38.0 41.0 34.0 41.0 16-17 38.46631838461765 40.0 38.0 41.0 34.0 41.0 18-19 38.457870626059346 40.0 38.0 41.0 34.0 41.0 20-21 38.39206055433114 40.0 38.0 41.0 34.0 41.0 22-23 38.277352894838444 40.0 38.0 41.0 34.0 41.0 24-25 38.155133077214735 40.0 37.5 41.0 33.0 41.0 26-27 37.943788835468325 40.0 37.0 41.0 33.0 41.0 28-29 37.96004878834204 40.0 37.0 41.0 33.0 41.0 30-31 37.839351139289164 40.0 37.0 41.0 33.0 41.0 32-33 37.63758208577953 40.0 37.0 41.0 32.5 41.0 34-35 37.485553075933566 40.0 36.0 41.0 32.0 41.0 36-37 37.38179699686806 40.0 36.0 41.0 32.0 41.0 38-39 37.29225827003949 40.0 36.0 41.0 31.0 41.0 40-41 37.1366679536078 39.5 36.0 41.0 31.0 41.0 42-43 36.941899282717884 39.0 36.0 41.0 30.5 41.0 44-45 36.73030919481923 39.0 35.0 41.0 30.0 41.0 46-47 36.47205387147094 39.0 35.0 41.0 29.5 41.0 48-49 36.2090403862901 39.0 35.0 40.0 28.5 41.0 50-51 35.99156747697789 39.0 35.0 40.0 28.0 41.0 52-53 35.770588836160854 38.5 35.0 40.0 27.0 41.0 54-55 35.544762351605016 38.0 34.0 40.0 27.0 41.0 56-57 35.32570131077551 38.0 34.0 40.0 26.0 41.0 58-59 35.04851063977133 38.0 34.0 40.0 26.0 41.0 60-61 34.72312148435674 37.5 33.0 40.0 25.5 41.0 62-63 34.37328985434481 37.0 33.0 40.0 25.0 41.0 64-65 34.03716535923836 36.0 32.5 39.0 24.0 41.0 66-67 33.667629506385936 36.0 32.0 39.0 23.0 40.5 68-69 33.26660370465913 35.0 31.5 39.0 22.5 40.0 70-71 32.88768276151019 35.0 31.0 38.0 22.0 40.0 72-73 32.45949857772807 35.0 31.0 37.5 21.5 39.5 74-75 32.0648071233057 35.0 30.5 37.0 20.5 39.0 76-77 30.58825876865704 33.5 28.5 35.5 20.0 37.5 78-79 31.46859955990076 34.0 30.0 36.0 20.0 38.0 80-81 31.435175217322804 34.0 30.0 35.5 20.0 37.0 82-83 31.161351946080053 34.0 30.0 35.0 20.0 37.0 84-85 30.842606176971138 34.0 30.0 35.0 19.5 36.5 86-87 30.549635818745596 34.0 29.0 35.0 19.0 36.0 88-89 30.267231478002827 34.0 29.0 35.0 18.0 36.0 90-91 29.985205601829648 34.0 29.0 35.0 18.0 35.0 92-93 29.7525046009588 34.0 29.0 35.0 17.0 35.0 94-95 29.52663725746498 33.5 29.0 35.0 16.5 35.0 96-97 29.288808459877735 33.0 27.0 35.0 15.0 35.0 98-99 29.08327345883029 33.0 27.0 35.0 14.0 35.0 100 27.313935841079505 31.0 24.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3007032369293192 1101 2 0.20076471768024362 1101 3 0.1773261678803948 1101 4 0.12415192728436608 1101 5 0.08055596744715388 1101 6 0.09371055833262432 1101 7 0.14833588705899103 1101 8 0.04666166929612814 1101 9 0.07686741362173422 1101 10-11 0.08111781616672431 1101 12-13 0.04640876684150186 1101 14-15 0.017149920068121105 1101 16-17 0.10884111286767251 1101 18-19 0.022833038705051933 1101 20-21 -0.04259654739065866 1101 22-23 0.013102298413521396 1101 24-25 -0.05158124161075506 1101 26-27 -0.028520151461812304 1101 28-29 0.05358527988668271 1101 30-31 0.02772884219498195 1101 32-33 0.09111910021944425 1101 34-35 0.013237943407375496 1101 36-37 -0.07911571710476295 1101 38-39 -0.10143383082651525 1101 40-41 -0.04759856787589456 1101 42-43 -0.05091879768308161 1101 44-45 -0.09027213122951139 1101 46-47 -0.11468193504325086 1101 48-49 -0.20096156865383108 1101 50-51 -0.23731358729735064 1101 52-53 -0.19784882828597006 1101 54-55 -0.311808169515551 1101 56-57 -0.29238422295642863 1101 58-59 -0.2762891267047749 1101 60-61 -0.38596162733826134 1101 62-63 -0.5332256062097187 1101 64-65 -0.4284467635149696 1101 66-67 -0.5477236715884857 1101 68-69 -0.47330648486475013 1101 70-71 -0.5409802381200493 1101 72-73 -0.6058074775293001 1101 74-75 -0.8109560507738394 1101 76-77 -0.6989406891681433 1101 78-79 -0.5076606485780459 1101 80-81 -0.5930740891848139 1101 82-83 -0.6823009061553265 1101 84-85 -0.49447593005367096 1101 86-87 -0.4986771122528104 1101 88-89 -0.7129129989082621 1101 90-91 -0.8823689550572666 1101 92-93 -0.9682054515734002 1101 94-95 -1.1188169534514607 1101 96-97 -1.23546991770122 1101 98-99 -1.2497762825376704 1101 100 -1.3790652976566236 1102 1 0.2414125333583037 1102 2 0.18859140681232844 1102 3 0.08759264479063233 1102 4 0.13250966176713774 1102 5 0.12988786670175756 1102 6 0.13299436414854426 1102 7 0.16819283361638782 1102 8 0.09409600430345932 1102 9 0.1656569257623275 1102 10-11 0.1686514870265725 1102 12-13 0.19797398470696947 1102 14-15 0.18898853986276976 1102 16-17 0.21044054687006764 1102 18-19 0.17752133106080947 1102 20-21 0.12669085725401175 1102 22-23 0.11483668849383122 1102 24-25 0.09417473053649417 1102 26-27 0.09004762375316488 1102 28-29 0.141861209175147 1102 30-31 0.14501862424773293 1102 32-33 0.1759747790076176 1102 34-35 0.11012652421471358 1102 36-37 0.1693704573948125 1102 38-39 0.1166834773586416 1102 40-41 0.021627084139176134 1102 42-43 -0.018915463313724956 1102 44-45 -0.09521847596458599 1102 46-47 -0.13743021333086602 1102 48-49 -0.03391354882755593 1102 50-51 -0.033871048596310516 1102 52-53 -0.038104794980448276 1102 54-55 -0.05745583600226922 1102 56-57 0.077082577848401 1102 58-59 0.0859019738279656 1102 60-61 0.05624240989325813 1102 62-63 -0.10521240505154594 1102 64-65 -0.04716789790941078 1102 66-67 -0.05332468962080128 1102 68-69 -0.22944971279602555 1102 70-71 -0.2871118380175304 1102 72-73 -0.4945167657791245 1102 74-75 -0.6286838948027835 1102 76-77 -0.3184589504976394 1102 78-79 -0.29802819259553814 1102 80-81 -0.3155372557178886 1102 82-83 -0.4701284813626678 1102 84-85 -0.44470303795855415 1102 86-87 -0.5019467299590445 1102 88-89 -0.5091991848586517 1102 90-91 -0.47291903057814366 1102 92-93 -0.5931117320748349 1102 94-95 -0.6232200658930473 1102 96-97 -0.6781861745921596 1102 98-99 -0.7962836465046692 1102 100 -0.907051643918475 1103 1 0.29733556567072483 1103 2 0.19224793732110612 1103 3 0.1892268677277471 1103 4 0.13667682995295394 1103 5 0.1393971256570694 1103 6 0.14731820691591224 1103 7 0.10926205420091861 1103 8 0.13571799017339004 1103 9 0.2098986652860475 1103 10-11 0.16404731329304667 1103 12-13 0.20493507046856507 1103 14-15 0.26224498575158606 1103 16-17 0.2623204230039491 1103 18-19 0.2106571570354916 1103 20-21 0.13570498003319642 1103 22-23 0.11565051729161269 1103 24-25 0.1060310991001785 1103 26-27 0.027167609017617167 1103 28-29 0.205513335433956 1103 30-31 0.33781626218372907 1103 32-33 0.3393238965162908 1103 34-35 0.3105141985154134 1103 36-37 0.24788419172461573 1103 38-39 0.2819087324039202 1103 40-41 0.19745012265220652 1103 42-43 0.20575166475055795 1103 44-45 0.253536571141737 1103 46-47 0.2606373878027455 1103 48-49 0.27600408349142924 1103 50-51 0.23487824471761343 1103 52-53 0.17177481306866582 1103 54-55 0.19378268832779355 1103 56-57 0.1985496034204033 1103 58-59 0.1373703762979872 1103 60-61 0.08548143209505099 1103 62-63 0.08364238053533768 1103 64-65 0.13761819961509048 1103 66-67 0.06656141082162748 1103 68-69 0.023762289377827983 1103 70-71 0.03499772476719443 1103 72-73 0.07953686759031342 1103 74-75 -2.7105700684160183E-4 1103 76-77 0.008235409144479178 1103 78-79 0.04682959481140614 1103 80-81 0.07138180273918238 1103 82-83 -0.014363154025677005 1103 84-85 -0.024822545412039432 1103 86-87 0.010727114814397254 1103 88-89 0.0024148587716901204 1103 90-91 -0.01749724385690854 1103 92-93 -0.15912327795745185 1103 94-95 -0.24785441284216958 1103 96-97 -0.16838808620077472 1103 98-99 -0.22636777406195918 1103 100 -0.14887414626193873 1104 1 0.2695685014851499 1104 2 0.14322878900704694 1104 3 0.1412673606422672 1104 4 0.04851702333329655 1104 5 0.08036009903602093 1104 6 0.021716404354023666 1104 7 0.08114534746988511 1104 8 0.06900701833897926 1104 9 0.11245039742838259 1104 10-11 0.08413959526586012 1104 12-13 0.0893565030097605 1104 14-15 0.08368578534268778 1104 16-17 0.10316493306914509 1104 18-19 0.13060954851015083 1104 20-21 0.024315538724707153 1104 22-23 0.042736821972788164 1104 24-25 -0.05541168696947807 1104 26-27 -0.11913815467178068 1104 28-29 0.11574130058800591 1104 30-31 0.13973103778977958 1104 32-33 0.08161881492559075 1104 34-35 0.08653260357401393 1104 36-37 0.12407356785875123 1104 38-39 0.007713591017981969 1104 40-41 -0.019601731134052613 1104 42-43 -0.01347973544897485 1104 44-45 0.11460739468218151 1104 46-47 0.2770653980431632 1104 48-49 0.053055568997947944 1104 50-51 -0.06028825120117176 1104 52-53 -0.009696531420203769 1104 54-55 0.09383597728899673 1104 56-57 0.13126997419998077 1104 58-59 0.07067374505313495 1104 60-61 -0.13233806173235507 1104 62-63 -0.11863007281031912 1104 64-65 -0.0701677361167441 1104 66-67 -0.13572114231666887 1104 68-69 -0.13954254133624744 1104 70-71 -0.06980659593526894 1104 72-73 -0.0897322533817615 1104 74-75 -0.30855941557291544 1104 76-77 -0.18245654383177978 1104 78-79 -0.16509630730653768 1104 80-81 -0.16331210100365823 1104 82-83 -0.21719851736153473 1104 84-85 -0.264765328133695 1104 86-87 -0.5253712739059786 1104 88-89 -0.2566939265971122 1104 90-91 -0.33675230323922634 1104 92-93 -0.3324025746101249 1104 94-95 -0.2797463774248179 1104 96-97 -0.49424662437576217 1104 98-99 -0.3896208224137432 1104 100 -0.38076438104584653 1105 1 0.2802907920515132 1105 2 0.1612798774904789 1105 3 0.12008067572853776 1105 4 0.1291920988000541 1105 5 0.08535037440528725 1105 6 0.09958426465529868 1105 7 0.09388271985983465 1105 8 0.06657758984424333 1105 9 0.0954246358028854 1105 10-11 0.14918494119428516 1105 12-13 0.14912419879103567 1105 14-15 0.25626018576691223 1105 16-17 0.17620357962452005 1105 18-19 0.2228059646419993 1105 20-21 0.08292237215108855 1105 22-23 0.21673821520523262 1105 24-25 0.02071166844807948 1105 26-27 0.13645313746073384 1105 28-29 0.16294717785509505 1105 30-31 0.2498535467280405 1105 32-33 0.1843193144142461 1105 34-35 0.13190076442484155 1105 36-37 0.11631938939654418 1105 38-39 0.027829002046260598 1105 40-41 0.03563203054415709 1105 42-43 0.04526922086181884 1105 44-45 0.10368089510459555 1105 46-47 0.027671190662317713 1105 48-49 0.05386680576347658 1105 50-51 0.12873040922064405 1105 52-53 -0.10054121564554919 1105 54-55 -0.15217643005528458 1105 56-57 -0.060578792558743544 1105 58-59 -0.1431691556370609 1105 60-61 0.047271668497657515 1105 62-63 -0.03040980374847635 1105 64-65 -0.08428780113426626 1105 66-67 0.042807432946702306 1105 68-69 0.05983659739577263 1105 70-71 -0.04938651823702145 1105 72-73 -0.07884264317394951 1105 74-75 -0.022868538610488542 1105 76-77 -0.0839247078116152 1105 78-79 0.006793004821997073 1105 80-81 0.05531209018867855 1105 82-83 0.03832706558108612 1105 84-85 0.12074801873671959 1105 86-87 -0.0611572562748357 1105 88-89 -0.07596236428289771 1105 90-91 0.01517985984472503 1105 92-93 -0.0986621407318502 1105 94-95 -0.03146263888481471 1105 96-97 -0.0123425070939156 1105 98-99 -0.08607993697005512 1105 100 -0.030041662299293392 1106 1 0.26341809410177675 1106 2 0.1401283491772105 1106 3 0.14790085981308465 1106 4 0.05983002354960121 1106 5 0.10626679742811973 1106 6 0.08747457004729853 1106 7 0.11468056990841546 1106 8 0.09249104746869108 1106 9 0.020836005619315756 1106 10-11 0.15423116639572498 1106 12-13 0.1598410368622396 1106 14-15 0.17184643138782718 1106 16-17 0.1450497120726837 1106 18-19 0.08395153423370516 1106 20-21 0.13014148147472326 1106 22-23 0.1329163590699025 1106 24-25 0.17887340928837148 1106 26-27 0.16079190517391595 1106 28-29 0.13557690517377097 1106 30-31 0.22961134639169245 1106 32-33 0.17267440653277788 1106 34-35 0.2656551069989206 1106 36-37 0.19605063902465503 1106 38-39 0.20309537773656672 1106 40-41 0.1857411198285277 1106 42-43 0.13410841077641322 1106 44-45 0.15470040787707262 1106 46-47 0.07010382907640178 1106 48-49 0.0806999557597976 1106 50-51 0.016566604952835462 1106 52-53 0.17408023691205443 1106 54-55 0.10118329865318998 1106 56-57 0.14140769141101828 1106 58-59 0.10221170741524332 1106 60-61 0.07630711816525348 1106 62-63 0.09921548202420638 1106 64-65 -0.04896938007088636 1106 66-67 0.12227871347682395 1106 68-69 0.0500926162093549 1106 70-71 -0.012123994449936504 1106 72-73 -0.0386218633031703 1106 74-75 0.13613739025303317 1106 76-77 0.13078882353428511 1106 78-79 0.03599605695909247 1106 80-81 0.1153525467719625 1106 82-83 0.04024614339266819 1106 84-85 0.10072245036511518 1106 86-87 0.07335610369875312 1106 88-89 0.056378392964500534 1106 90-91 0.00845283051230794 1106 92-93 0.12380551537473039 1106 94-95 0.31466562055629055 1106 96-97 0.20704402383247 1106 98-99 0.023519995237133884 1106 100 -0.0342505732731162 1107 1 0.31613065435911736 1107 2 0.19336506086602867 1107 3 0.17414055032178766 1107 4 0.12353528921651247 1107 5 0.104686313400002 1107 6 0.09017256056418432 1107 7 0.07771854357553565 1107 8 0.1367916164598455 1107 9 0.0994063157635523 1107 10-11 0.11605707289454159 1107 12-13 0.20860428882830462 1107 14-15 0.21195273238323864 1107 16-17 0.25481944678085 1107 18-19 0.19054772801315067 1107 20-21 0.18733896495618296 1107 22-23 0.22111844041913997 1107 24-25 0.21324373342124403 1107 26-27 0.15508915661720835 1107 28-29 0.22884786156365067 1107 30-31 0.2794838638040673 1107 32-33 0.21525930670311055 1107 34-35 0.19948191325052989 1107 36-37 0.24624762107038833 1107 38-39 0.23315833084862447 1107 40-41 0.1471315189295339 1107 42-43 0.20338303633197086 1107 44-45 0.30794824497321827 1107 46-47 0.23660548119410407 1107 48-49 0.3314796323347977 1107 50-51 0.18862203293658553 1107 52-53 0.2938017699741664 1107 54-55 0.35057129559597655 1107 56-57 0.37271103763349345 1107 58-59 0.2976852197538804 1107 60-61 0.20868308889138376 1107 62-63 0.1876627872138812 1107 64-65 0.228498489900673 1107 66-67 0.29360428093070823 1107 68-69 0.3108626021636667 1107 70-71 0.334490976227805 1107 72-73 0.264346803196851 1107 74-75 0.12181287363771531 1107 76-77 0.09895135210587469 1107 78-79 0.2593336432338269 1107 80-81 0.36979145750900244 1107 82-83 0.42009639308568936 1107 84-85 0.2644111599890273 1107 86-87 0.2619873848786831 1107 88-89 0.12344515872089801 1107 90-91 0.15605544786739145 1107 92-93 0.17732670151008634 1107 94-95 0.3248150982749394 1107 96-97 0.2532086532359621 1107 98-99 0.26428305448959577 1107 100 0.38054391520220676 1108 1 0.259357236151633 1108 2 0.12170540721302814 1108 3 0.11709186974397312 1108 4 0.0902722549572772 1108 5 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-0.07163311273154704 2316 66-67 -0.013364580351520772 2316 68-69 0.12804341468510927 2316 70-71 -0.08706068725268068 2316 72-73 -0.2525038264849755 2316 74-75 0.13456873792785728 2316 76-77 0.05924803125508049 2316 78-79 -0.2105787675128603 2316 80-81 -0.02773391695387062 2316 82-83 -0.0819428462781353 2316 84-85 0.11585884749031194 2316 86-87 0.12637679624777576 2316 88-89 0.22562939197402798 2316 90-91 0.2240644585440954 2316 92-93 0.3072243967768067 2316 94-95 0.2957612863918726 2316 96-97 0.46857471623512126 2316 98-99 0.39270829694533305 2316 100 0.08242983638178103 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 3.0 10 11.0 11 14.0 12 43.0 13 128.0 14 393.0 15 1043.0 16 2376.0 17 4987.0 18 8695.0 19 12944.0 20 17512.0 21 22640.0 22 28419.0 23 31916.0 24 34098.0 25 37514.0 26 42273.0 27 49957.0 28 59277.0 29 70275.0 30 82946.0 31 98646.0 32 119801.0 33 149711.0 34 194958.0 35 265257.0 36 383432.0 37 570663.0 38 522503.0 39 75543.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.438743273737195 19.611386911994448 13.399965283804896 23.54990453046346 2 15.347812405999925 21.12823654819943 39.74659312539766 23.777357920402988 3 17.406945076862613 27.246086750267057 30.456847343006878 24.89012082986345 4 12.007597071284005 17.23998469505238 37.876871350142224 32.87554688352138 5 12.507176311144075 38.00127701980697 35.19602323289614 14.295523436152818 6 30.68225691569721 37.52338589294377 18.200708528064283 13.593648663294736 7 27.13268940108761 32.63552269142672 22.70389216010471 17.52789574738096 8 26.335508337361546 35.60300613165538 20.263445112196692 17.798040418786385 9 26.828844332640234 15.14131825476933 20.461186605886112 37.568650806704326 10-11 24.88631738265002 26.210723120997088 28.847259558559124 20.055699937793772 12-13 25.322997505495255 24.046104708256518 28.926334546189892 21.704563240058338 14-15 22.696970845930785 25.299668332707466 26.604003587969395 25.399357233392355 16-17 22.147387126536046 28.18513774076305 27.25149191675356 22.415983215947342 18-19 22.00670787468892 27.109169864149262 29.364022702616978 21.520099558544835 20-21 22.769396178590966 26.460868300587965 29.329019993490167 21.4407155273309 22-23 22.58006880229641 26.298613046812918 29.141650666468145 21.97966748442253 24-25 22.28826020361259 26.469792341337012 29.314076997296905 21.927870457753496 26-27 22.2448639688862 26.55317091987165 29.249537479323777 21.952427631918376 28-29 22.2597788213907 26.514649295561277 29.386924059125242 21.838647823922784 30-31 22.52443277593178 26.51470398805509 29.300043906972622 21.660819329040514 32-33 22.35715328243466 26.608797516668286 29.231885029041056 21.802164171856 34-35 22.515191594062532 26.70917444678393 29.12525124542763 21.650382713725907 36-37 22.58297688008385 26.634320691232478 29.004744899743518 21.77795752894015 38-39 22.46264686180313 26.828224821940967 28.936746429426602 21.7723818868293 40-41 22.48684540866238 26.664525456450033 29.071188025382867 21.77744110950472 42-43 22.387285679160275 26.755794584448733 28.855887741073065 22.001031995317927 44-45 22.282696492174907 26.993802570496996 28.729197107970123 21.99430382935798 46-47 22.324136253336256 26.869128821162953 28.52586716624261 22.280867759258186 48-49 22.3954276176534 26.98453565853958 28.433195481984818 22.1868412418222 50-51 22.40150605111058 27.17003882171204 28.24569562406482 22.18275950311256 52-53 22.515986657326472 27.216446545334165 28.039784935438732 22.227781861900628 54-55 22.379296648697988 27.190739811545317 28.225938542206652 22.20402499755004 56-57 22.47856344570806 27.24465070860743 27.98538375435981 22.2914020913247 58-59 22.485182718339463 27.182667437923065 28.162314267822076 22.1698355759154 60-61 22.616996450321732 27.275141880645553 28.10321545340253 22.004646215630185 62-63 22.432874718000832 27.35336086560607 28.128924591227822 22.08483982516528 64-65 22.35854721395357 27.096824238126043 28.120805555055355 22.423822992865027 66-67 22.30019574181473 27.217340941378747 27.967168011580018 22.515295305226505 68-69 22.254929801838244 27.262548743913506 28.062364504041504 22.420156950206753 70-71 22.288753920958058 27.061306755743423 28.08282919469577 22.56711012860275 72-73 22.25894459137625 27.40889179635702 27.75186029284699 22.580303319419738 74-75 22.274989507336553 27.43249719849349 27.68473667223967 22.607776621930288 76-77 22.197399829873408 27.319879377370597 27.902267760144706 22.58045303261129 78-79 22.21901112083869 27.2465396294162 27.89409506737887 22.64035418236624 80-81 22.140166392900348 27.560670246434704 27.534214152728882 22.764949207936066 82-83 22.336086047736227 27.469343433372696 27.644909712110437 22.549660806780643 84-85 22.175049960343436 27.566766760526306 27.58003179452012 22.678151484610137 86-87 22.11608540961008 27.507735442917202 27.69822759699888 22.67795155047384 88-89 21.996937820650906 27.402297361788076 27.748359645726424 22.8524051718346 90-91 22.226249763600833 27.435933227712837 27.648727116040988 22.689089892645338 92-93 22.12119333425232 27.519813053167073 27.59755170858793 22.761441903992676 94-95 21.998613144576908 27.300391014257524 27.639987018146538 23.06100882301903 96-97 22.038376775074333 27.362625735965253 27.630024351941046 22.968973137019365 98-99 21.949257276796452 27.259464541099177 27.575071901715305 23.21620628038907 100 21.149259288120035 27.684424703671954 27.848515545858334 23.317800462349673 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 961.0 1 758.0 2 1543.0 3 2217.0 4 1401.0 5 1006.0 6 1489.0 7 2787.5 8 4347.5 9 5070.5 10 4328.5 11 3393.0 12 3432.0 13 3631.5 14 3874.0 15 4134.0 16 4342.0 17 4592.5 18 4861.5 19 5198.5 20 5653.5 21 6391.5 22 7340.5 23 8699.5 24 10530.0 25 12751.0 26 15598.5 27 19107.0 28 23126.0 29 27543.0 30 32532.0 31 37753.0 32 43569.0 33 50342.5 34 57308.5 35 63394.5 36 70877.0 37 77656.0 38 82188.5 39 87272.0 40 89661.5 41 90824.0 42 92902.0 43 95459.0 44 98097.5 45 100979.0 46 103391.0 47 104589.5 48 106712.5 49 108554.0 50 108719.5 51 106210.5 52 102032.0 53 98342.5 54 94205.0 55 88107.5 56 80658.5 57 72558.5 58 64596.5 59 56182.5 60 46892.5 61 38944.5 62 32159.0 63 25282.5 64 18873.5 65 14167.0 66 10820.5 67 8239.0 68 6345.5 69 4886.0 70 3755.5 71 2852.0 72 2218.0 73 1806.5 74 1476.5 75 1133.0 76 831.5 77 574.0 78 409.5 79 318.0 80 214.5 81 148.5 82 102.5 83 69.5 84 51.0 85 40.0 86 26.0 87 14.0 88 10.0 89 7.5 90 5.0 91 2.0 92 1.5 93 1.5 94 0.5 95 0.5 96 0.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2591772464192162 2 0.08133698755360572 3 0.0013850487450592713 4 0.0010387865587944535 5 3.8088840489129965E-4 6 9.002816842885264E-4 7 0.0 8 7.271505911561175E-4 9 0.0 10-11 3.6357529557805876E-4 12-13 0.001281170089179826 14-15 1.7313109313240895E-5 16-17 1.9044420244564982E-4 18-19 0.0012465438705533442 20-21 0.0015581798381916804 22-23 0.0 24-25 1.7313109313240895E-5 26-27 2.0775731175889074E-4 28-29 0.0016447453847578848 30-31 0.002112199336215389 32-33 0.0031336727856966016 34-35 0.004207085563117537 36-37 0.001454301182312235 38-39 0.003756944720973274 40-41 0.007271505911561175 42-43 0.012984831984930669 44-45 0.008846998859066096 46-47 0.007011809271862562 48-49 0.007358071458127379 50-51 0.00581720472924894 52-53 0.003739631611660033 54-55 0.006301971790019685 56-57 0.003549187409214383 58-59 0.0019563813523962212 60-61 0.008950877514945542 62-63 0.011894106098196494 64-65 0.008206413814476184 66-67 0.008743120203186652 68-69 0.015408667288784395 70-71 0.004795731279767727 72-73 0.013919739887845678 74-75 0.008760433312499892 76-77 0.004691852623888282 78-79 0.014993152665266612 80-81 0.006284658680706444 82-83 0.01632626208238616 84-85 0.024065221945404842 86-87 0.01608387855200079 88-89 0.01727848309461441 90-91 0.03182149491773676 92-93 0.025865785313981895 94-95 0.03040181995405101 96-97 0.018542340074480995 98-99 0.018576966293107476 100 0.019390682430829802 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2887985.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.35268342723423 #Duplication Level Percentage of deduplicated Percentage of total 1 78.60950757460576 52.159517704685875 2 13.984821065984793 18.558608099556107 3 3.7893314042917394 7.542969212095402 4 1.3903757728053028 3.690206539913856 5 0.6834124018116362 2.267312337382665 6 0.39867274017063503 1.5871803671766094 7 0.2609770304961694 1.2121568400404386 8 0.15320874714979668 0.8132649198330908 9 0.1176895652904684 0.7028116621566538 >10 0.5673457908117009 6.461882465824548 >50 0.029126295002389065 1.328146543405479 >100 0.014353695512834904 1.7750611057028718 >500 7.439469896260789E-4 0.31098613826122984 >1k 2.4798232986972425E-4 0.3901724234595001 >5k 6.199558246743106E-5 0.2993447259372464 >10k+ 1.2399116493486213E-4 0.9003789145684279 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11681 0.4044688597759337 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10207 0.3534298135204996 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7277 0.251974992944908 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3802 0.13164888321788376 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 1.2119176519268624E-4 16-17 0.0 0.0 0.0 0.0 1.3850487450592713E-4 18-19 0.0 0.0 0.0 3.462621862648178E-5 1.3850487450592713E-4 20-21 0.0 0.0 0.0 6.925243725296357E-5 1.3850487450592713E-4 22-23 0.0 0.0 0.0 1.3850487450592716E-4 1.9044420244564982E-4 24-25 0.0 0.0 0.0 2.423835303853725E-4 2.423835303853725E-4 26-27 0.0 0.0 0.0 4.328277328310223E-4 2.423835303853725E-4 28-29 0.0 0.0 0.0 0.0013677356357460306 2.423835303853725E-4 30-31 0.0 0.0 0.0 0.00444946909350291 2.770097490118543E-4 32-33 0.0 0.0 0.0 0.011011137523221208 3.462621862648179E-4 34-35 0.0 0.0 0.0 0.019650379070528412 3.462621862648179E-4 36-37 0.0 0.0 0.0 0.0361151460274205 3.462621862648179E-4 38-39 0.0 0.0 0.0 0.061582729827197855 3.6357529557805876E-4 40-41 0.0 0.0 0.0 0.09435644575716287 3.8088840489129965E-4 42-43 0.0 0.0 0.0 0.12410036755731071 3.8088840489129965E-4 44-45 0.0 0.0 0.0 0.15683945726864923 3.8088840489129965E-4 46-47 0.0 0.0 0.0 0.19665960868910332 3.8088840489129965E-4 48-49 0.0 0.0 0.0 0.23725885002865318 4.155146235177814E-4 50-51 0.0 0.0 0.0 0.28466214332830675 4.155146235177814E-4 52-53 0.0 0.0 0.0 0.3314075384740572 4.501408421442632E-4 54-55 0.0 0.0 0.0 0.38457609717501995 4.84767060770745E-4 56-57 0.0 0.0 0.0 0.4425923264836902 4.84767060770745E-4 58-59 0.0 0.0 0.0 0.5065815785054285 5.020801700839859E-4 60-61 0.0 0.0 0.0 0.5734101804545384 5.193932793972268E-4 62-63 0.0 0.0 0.0 0.6421951637560444 5.193932793972268E-4 64-65 0.0 0.0 0.0 0.709006452595841 5.193932793972268E-4 66-67 0.0 0.0 0.0 0.7846474271853905 5.193932793972268E-4 68-69 0.0 0.0 0.0 0.8704165707231859 5.193932793972268E-4 70-71 0.0 0.0 0.0 0.9614662126015198 5.540194980237085E-4 72-73 0.0 0.0 0.0 1.0507845435485295 6.57898153903154E-4 74-75 0.0 0.0 0.0 1.1427171540018386 6.57898153903154E-4 76-77 0.0 0.0 0.0 1.2467862540837298 6.57898153903154E-4 78-79 0.0 0.0 0.0 1.359944736555072 6.752112632163949E-4 80-81 0.0 0.0 0.0 1.4798899578772051 7.098374818428766E-4 82-83 0.0 0.0 0.0 1.6014799245840958 7.617768097825993E-4 84-85 0.0 0.0 0.0 1.7240740516311546 8.483423563488037E-4 86-87 0.0 0.0 0.0 1.8513773444114148 8.656554656620446E-4 88 0.0 0.0 0.0 1.9506680263228513 8.656554656620446E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5575 0.0 49.392525 1 TACCTGG 3205 0.0 40.345936 2 GTACCTG 3310 0.0 39.601357 1 GTATCAA 9555 0.0 37.849308 1 TATAACG 295 0.0 35.066772 2 ACCTGGG 3895 0.0 32.33118 3 TCAACGC 11875 0.0 30.150919 4 CAACGCA 12020 0.0 29.825262 5 ATCAACG 12050 0.0 29.753067 3 TATCAAC 12370 0.0 29.155521 2 AACGCAG 12540 0.0 28.814795 6 GTACATG 10685 0.0 25.682762 1 ACGCAGA 14115 0.0 25.433096 7 TAACGCA 370 0.0 25.398464 4 CGCAGAG 14605 0.0 24.611982 8 TACATGG 11110 0.0 24.589924 2 ACATGGG 10935 0.0 24.192673 3 CCTGGGG 3535 0.0 23.526812 4 GTATAAC 790 0.0 22.680351 1 CATGGGG 6805 0.0 22.164408 4 >>END_MODULE