FastQCFastQC Report
Mon 6 Jun 2016
ERR1211567_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211567_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5873669
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154820.2635831198523444No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130240.22173534123220087No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85160.14498603853911413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78150.049.482871
GTATCAA139100.039.6330381
TCAACGC164700.031.9522344
CAACGCA165950.031.792985
ATCAACG166400.031.6543063
AACGCAG172900.030.674396
TATCAAC178550.029.8775082
GTACATG178750.027.7812821
ACGCAGA199300.026.4699527
TACATGG182700.026.4437522
CGCAGAG206150.025.5229078
ACATGGG182750.024.91423
GTACCTG48800.024.2562621
CATGGGG118400.023.2156584
TACCTGG46650.022.7901922
GCAGAGT234600.022.1472029
AGAGTAC211700.020.12893110-11
GAGTACT147600.019.6412112-13
CAGAGTA223400.019.6320410-11
ATGGGGG69750.018.9947535
Produced by FastQC (version 0.11.5)