FastQCFastQC Report
Mon 6 Jun 2016
ERR1211567_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211567_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5873669
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182540.3107767904524412No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157970.2689460369659918No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109640.18666356582231652No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86450.048.2683031
GTATCAA148800.038.1828081
TCAACGC184250.030.2725724
ATCAACG184350.030.181483
CAACGCA185900.030.003885
AACGCAG192400.029.040076
TATCAAC198400.028.3517762
GTACATG193250.027.2287981
TACATGG194850.026.382172
ACATGGG195200.025.4464453
TACCTGG46450.025.2118322
ACGCAGA223250.024.9006397
GTACCTG49800.024.1431871
CGCAGAG230400.024.0055248
CATGGGG123850.023.9408444
GCAGAGT260100.021.1195169
GAGTACT165450.019.88062112-13
AGAGTAC237700.019.52170410-11
ATGGGGG73850.019.1523515
TATAACG4800.018.6167182
Produced by FastQC (version 0.11.5)