FastQCFastQC Report
Mon 6 Jun 2016
ERR1211566_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211566_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6988614
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193540.27693617074859195No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167830.2401477603427518No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111370.1593592091364611No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104400.044.7595561
GTATCAA169950.036.790441
TCAACGC199050.030.501094
CAACGCA201500.030.1045425
ATCAACG203100.029.8459533
AACGCAG210850.028.8991496
GTACATG229450.028.7913911
TATCAAC216700.028.3540782
TACATGG238200.027.3551622
ACATGGG240450.026.206543
ACGCAGA241350.024.974577
CATGGGG157300.024.7950174
CGCAGAG252200.023.789558
TACCTGG56250.022.8334432
GTACCTG61000.022.1852681
ATGGGGG90350.020.802365
GCAGAGT284850.020.2873929
GAGTACA169650.019.7062951
GAGTACT169800.019.52299112-13
AGAGTAC257550.018.52628310-11
Produced by FastQC (version 0.11.5)