FastQCFastQC Report
Mon 6 Jun 2016
ERR1211566_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211566_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6988614
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236520.3384362049470754No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207780.29731217091114204No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145050.20755188367822286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121300.044.9698371
GTATCAA196150.035.7654231
TCAACGC245250.028.1841624
CAACGCA246550.027.9976395
ATCAACG249050.027.7733963
GTACATG252750.027.513741
AACGCAG254950.027.0759586
TATCAAC263600.026.4231242
TACATGG263150.026.2181022
ACATGGG264200.025.2381363
CATGGGG168650.024.0729264
TACCTGG56000.023.5989572
ACGCAGA293400.023.2872527
CGCAGAG301600.022.6541088
GTACCTG60950.022.122851
ATGGGGG89650.020.9135235
GCAGAGT344050.019.6949359
AGAGTAC310600.019.37264410-11
GAGTACT205800.019.1568612-13
TAACGCA6950.018.931054
Produced by FastQC (version 0.11.5)