##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211566_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6988614 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.91110655131332 33.0 31.0 34.0 30.0 34.0 2 32.19254719176077 33.0 31.0 34.0 30.0 34.0 3 32.28337779136178 34.0 31.0 34.0 30.0 34.0 4 35.95094077309177 37.0 35.0 37.0 35.0 37.0 5 35.83326450709683 37.0 35.0 37.0 35.0 37.0 6 35.86548634679208 37.0 35.0 37.0 35.0 37.0 7 35.76216328445096 37.0 35.0 37.0 33.0 37.0 8 35.74595434802952 37.0 35.0 37.0 33.0 37.0 9 37.35949903657578 39.0 37.0 39.0 34.0 39.0 10-11 37.31583587246341 39.0 37.0 39.0 34.0 39.0 12-13 37.2149980668556 39.0 37.0 39.0 33.0 39.0 14-15 38.39937089099498 40.0 38.0 41.0 34.0 41.0 16-17 38.31650081117658 40.0 38.0 41.0 33.0 41.0 18-19 38.29757617175595 40.0 38.0 41.0 33.5 41.0 20-21 38.22244160287004 40.0 38.0 41.0 33.0 41.0 22-23 38.11156933263162 40.0 37.5 41.0 33.0 41.0 24-25 37.98848813226772 40.0 37.0 41.0 33.0 41.0 26-27 37.766370127181155 40.0 37.0 41.0 32.5 41.0 28-29 37.78249442593338 40.0 37.0 41.0 33.0 41.0 30-31 37.66643858138395 40.0 37.0 41.0 32.5 41.0 32-33 37.48530001800071 39.5 36.5 41.0 32.0 41.0 34-35 37.345820644837445 39.0 36.0 41.0 31.5 41.0 36-37 37.24640064539263 39.0 36.0 41.0 31.0 41.0 38-39 37.162468194694966 39.0 36.0 41.0 31.0 41.0 40-41 36.99771714391437 39.0 36.0 41.0 30.5 41.0 42-43 36.79421019675718 39.0 35.0 41.0 30.0 41.0 44-45 36.57728370747047 39.0 35.0 40.0 30.0 41.0 46-47 36.31406627980884 39.0 35.0 40.0 29.5 41.0 48-49 36.04927085685374 39.0 35.0 40.0 28.0 41.0 50-51 35.82332355170854 38.0 34.5 40.0 27.5 41.0 52-53 35.59510991735986 38.0 34.0 40.0 27.0 41.0 54-55 35.35558617202209 38.0 34.0 40.0 26.0 41.0 56-57 35.12206991257494 38.0 34.0 40.0 26.0 41.0 58-59 34.82685107519173 37.5 33.0 40.0 26.0 41.0 60-61 34.48777032184064 37.0 33.0 40.0 25.5 41.0 62-63 34.12295492353705 36.5 32.5 39.5 24.5 41.0 64-65 33.77607240863496 36.0 32.0 39.0 23.5 40.5 66-67 33.393956154968635 35.5 31.5 39.0 23.0 40.0 68-69 32.97927142635149 35.0 31.0 38.5 22.0 40.0 70-71 32.591243557020036 35.0 31.0 38.0 22.0 40.0 72-73 32.15502673348392 35.0 30.0 37.0 21.0 39.0 74-75 31.75271484445986 34.0 30.0 36.5 20.0 39.0 76-77 30.339359277819607 32.5 28.5 35.0 19.5 37.5 78-79 31.219817477399666 34.0 30.0 36.0 20.0 38.0 80-81 31.190443770395675 34.0 30.0 35.0 20.0 37.0 82-83 30.911850618734988 34.0 29.5 35.0 20.0 37.0 84-85 30.587441300950374 34.0 29.0 35.0 19.0 36.0 86-87 30.282748625120803 34.0 29.0 35.0 18.0 36.0 88-89 29.986885954783023 34.0 29.0 35.0 18.0 35.5 90-91 29.696005459737798 33.5 29.0 35.0 17.5 35.0 92-93 29.44550528903156 33.0 27.5 35.0 17.0 35.0 94-95 29.210261505357145 33.0 27.0 35.0 15.5 35.0 96-97 28.95494449972484 33.0 27.0 35.0 14.0 35.0 98-99 28.723581313834188 33.0 26.0 34.5 11.5 35.0 100 26.869346053452087 31.0 23.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11153130256865751 1101 2 0.06187460393048383 1101 3 0.06164967980419789 1101 4 0.08157543393145517 1101 5 0.061782093069041366 1101 6 0.03361166853419206 1101 7 0.02486428065785873 1101 8 0.06020924922798088 1101 9 0.016238554606815114 1101 10-11 0.060411436010916475 1101 12-13 0.017142456715461662 1101 14-15 -0.007344997313516899 1101 16-17 0.05251403559361023 1101 18-19 0.008016578432105348 1101 20-21 -0.08272055154727553 1101 22-23 -0.14695851126771942 1101 24-25 -0.11758795887188711 1101 26-27 -0.21960361156900632 1101 28-29 -0.08501176710591096 1101 30-31 -0.03854222280692454 1101 32-33 -0.035006257787820516 1101 34-35 -0.13982815454714626 1101 36-37 -0.16361341893195203 1101 38-39 -0.20805628012588784 1101 40-41 -0.20763483127833382 1101 42-43 -0.2665684450004946 1101 44-45 -0.2866922626074455 1101 46-47 -0.35354195071995775 1101 48-49 -0.24623076978959801 1101 50-51 -0.4177908531624297 1101 52-53 -0.3798255271678599 1101 54-55 -0.5612824894790336 1101 56-57 -0.6074152970344286 1101 58-59 -0.6052807494890189 1101 60-61 -0.6356142424265343 1101 62-63 -0.8133058232027466 1101 64-65 -0.6947028998137483 1101 66-67 -0.7551028918647091 1101 68-69 -0.838430536292627 1101 70-71 -0.7914137288633931 1101 72-73 -1.0763705494963425 1101 74-75 -1.050482914569077 1101 76-77 -0.9571427539408397 1101 78-79 -0.8981604540373773 1101 80-81 -0.7945211375707331 1101 82-83 -0.8751393876909574 1101 84-85 -0.9231395300354386 1101 86-87 -1.0581072738926274 1101 88-89 -1.1720593876135403 1101 90-91 -1.2125516074469083 1101 92-93 -1.3324451211085062 1101 94-95 -1.4291386075419652 1101 96-97 -1.574987023124418 1101 98-99 -1.5857862889705139 1101 100 -1.6124578444798168 1102 1 0.2284438982566499 1102 2 0.1522154060393106 1102 3 0.14701517199630132 1102 4 0.14236345149694074 1102 5 0.10241101701063116 1102 6 0.11140549469737948 1102 7 0.11014988185480945 1102 8 0.10551999604479079 1102 9 0.1250960080965271 1102 10-11 0.09445064860073416 1102 12-13 0.14773289287656155 1102 14-15 0.16846167700617087 1102 16-17 0.13787304904307263 1102 18-19 0.062404003192654045 1102 20-21 0.04111767829826363 1102 22-23 0.05718950370627596 1102 24-25 0.05041442206603364 1102 26-27 0.012485256533913969 1102 28-29 0.11114413168803594 1102 30-31 0.05946461226655941 1102 32-33 0.12008981994089396 1102 34-35 0.05879363467910537 1102 36-37 0.07125061857995973 1102 38-39 0.05466740352520816 1102 40-41 -0.067959638991276 1102 42-43 -0.1326773324181687 1102 44-45 -0.10223589997602289 1102 46-47 -0.08778599280748978 1102 48-49 -0.15694304680443594 1102 50-51 -0.08146268055252648 1102 52-53 -0.14243336280215857 1102 54-55 -0.15734252312800123 1102 56-57 -0.176095739127625 1102 58-59 -0.19078391496217506 1102 60-61 -0.20934002559516074 1102 62-63 -0.22225529508399688 1102 64-65 -0.2174815110547712 1102 66-67 -0.3136541842726288 1102 68-69 -0.3162436569612268 1102 70-71 -0.30198069982850484 1102 72-73 -0.36869779483620135 1102 74-75 -0.45479174408298917 1102 76-77 -0.3748997525013458 1102 78-79 -0.28456509605647895 1102 80-81 -0.33052591969264355 1102 82-83 -0.36649328293373884 1102 84-85 -0.5285517906509618 1102 86-87 -0.5454232320480479 1102 88-89 -0.510989261298306 1102 90-91 -0.48209732611155687 1102 92-93 -0.6075286665194284 1102 94-95 -0.5856734722880894 1102 96-97 -0.7754172318816579 1102 98-99 -0.7518103251056978 1102 100 -0.8278210893580642 1103 1 0.22001063461213022 1103 2 0.17103167135005748 1103 3 0.14974485111230962 1103 4 0.12166730904498735 1103 5 0.06833531144728511 1103 6 0.08533511186778497 1103 7 0.06367685257117017 1103 8 0.1063378501456782 1103 9 0.11633414594271585 1103 10-11 0.10454190365890526 1103 12-13 0.17942432344729298 1103 14-15 0.1435712719793898 1103 16-17 0.13847750625901512 1103 18-19 0.15738167696298433 1103 20-21 0.1350375447710661 1103 22-23 0.09945906460941245 1103 24-25 0.019943233943529037 1103 26-27 -0.05567851503592891 1103 28-29 0.11242302171450547 1103 30-31 0.24375844667103763 1103 32-33 0.18864706083761718 1103 34-35 0.1509107118788151 1103 36-37 0.11845833424962393 1103 38-39 0.14576166122468237 1103 40-41 0.11450419022238378 1103 42-43 0.0036133301417322627 1103 44-45 -0.017869680981092984 1103 46-47 0.0742550289509083 1103 48-49 0.04519419139650438 1103 50-51 0.14584466430383713 1103 52-53 -0.028132100660393178 1103 54-55 -0.056648187422958074 1103 56-57 -0.20049298828376294 1103 58-59 -0.17186130969049174 1103 60-61 -0.11997772121853956 1103 62-63 -0.13593638417955844 1103 64-65 -0.17208389768139654 1103 66-67 -0.18824865562709192 1103 68-69 -0.20810199653214312 1103 70-71 -0.23358867589914212 1103 72-73 -0.278551448336561 1103 74-75 -0.23720957294677802 1103 76-77 -0.09882340453910032 1103 78-79 -0.09513344701276338 1103 80-81 -0.13671018493456089 1103 82-83 -0.15779224792586888 1103 84-85 -0.1929431320314876 1103 86-87 -0.31965167942152917 1103 88-89 -0.39841054592404035 1103 90-91 -0.4535829022487299 1103 92-93 -0.40137901625401184 1103 94-95 -0.4196091406267115 1103 96-97 -0.4992604406641661 1103 98-99 -0.42869388743703496 1103 100 -0.4514075680083387 1104 1 0.22926421856496404 1104 2 0.10969039944284731 1104 3 0.14577647593738874 1104 4 0.11159874138156312 1104 5 0.08278961408259988 1104 6 0.09873216760070136 1104 7 0.1196991234613094 1104 8 0.12746759213328573 1104 9 0.14308996333652146 1104 10-11 0.13668001366105642 1104 12-13 0.10541123728395974 1104 14-15 0.10103659684912714 1104 16-17 0.06291776507527658 1104 18-19 0.10543004123626076 1104 20-21 0.016959027335182952 1104 22-23 0.012855966482227643 1104 24-25 -0.07184672595862196 1104 26-27 0.04989324585164212 1104 28-29 0.052306048900405244 1104 30-31 0.10909644960175058 1104 32-33 0.10246062918713505 1104 34-35 0.04424009537617479 1104 36-37 0.11322062265777788 1104 38-39 0.06540500816912242 1104 40-41 -0.002612445087216031 1104 42-43 -0.12961577341378927 1104 44-45 -0.03933279603973716 1104 46-47 0.10985021418556329 1104 48-49 -0.026128983142562845 1104 50-51 -0.11608366370555245 1104 52-53 -0.12781309926341322 1104 54-55 -0.13379482469436965 1104 56-57 -0.07273743917640729 1104 58-59 -0.1710276443232388 1104 60-61 -0.20230677541726294 1104 62-63 -0.2198454867412707 1104 64-65 -0.1199906061797833 1104 66-67 -0.0625097376959971 1104 68-69 -0.27993863645222206 1104 70-71 -0.10033214345472175 1104 72-73 -0.06347159607873465 1104 74-75 -0.14802491110546612 1104 76-77 -0.09399903528577269 1104 78-79 0.04759084835731642 1104 80-81 0.12304434664460118 1104 82-83 0.05888539409536975 1104 84-85 -0.2384774904758551 1104 86-87 -0.20003094231534746 1104 88-89 -0.2404814334940113 1104 90-91 -0.14780244816613575 1104 92-93 -0.14908801071094402 1104 94-95 -0.25977421429037406 1104 96-97 -0.3927459636814099 1104 98-99 -0.41254928902104027 1104 100 -0.23804069165123565 1105 1 0.29065659408110633 1105 2 0.155356487314684 1105 3 0.09944215332631501 1105 4 0.05369080599290754 1105 5 0.07135659673284067 1105 6 0.004466010700852507 1105 7 0.02114182532761788 1105 8 0.07336057207041335 1105 9 0.0704250048733357 1105 10-11 0.11503687272823271 1105 12-13 0.1326336108630457 1105 14-15 0.19623027905112878 1105 16-17 0.15588328845956312 1105 18-19 0.13041678493001996 1105 20-21 0.06667472417985465 1105 22-23 0.027986126802815647 1105 24-25 -0.013707322959632506 1105 26-27 0.06652339719146738 1105 28-29 0.06438313615024072 1105 30-31 0.10083439722127707 1105 32-33 0.12083372640686463 1105 34-35 0.10531641182861051 1105 36-37 0.14868182250040007 1105 38-39 -0.03409153477821292 1105 40-41 0.045910445489745655 1105 42-43 0.0798574783834809 1105 44-45 0.13225547512735147 1105 46-47 0.19835640053922532 1105 48-49 0.18502733954067452 1105 50-51 0.19650802139103973 1105 52-53 0.06499211359078316 1105 54-55 0.025018124082599513 1105 56-57 0.08969265795366965 1105 58-59 0.14513358526429698 1105 60-61 0.09429341848984762 1105 62-63 0.043655980839638175 1105 64-65 0.08686109444423806 1105 66-67 0.029227375718832604 1105 68-69 0.021545342094896114 1105 70-71 0.026367872819051286 1105 72-73 0.02735225674076247 1105 74-75 -0.0017235134506528027 1105 76-77 0.042885710683219 1105 78-79 0.04800703134009865 1105 80-81 -0.010608998063336372 1105 82-83 0.025058935460386067 1105 84-85 0.03885363939576436 1105 86-87 0.10191752953777566 1105 88-89 -0.008015651757883546 1105 90-91 -0.08186670546382047 1105 92-93 -0.06465824014007637 1105 94-95 -0.050061898007022876 1105 96-97 2.5877113774441796E-5 1105 98-99 -0.008092166213565122 1105 100 -0.012635426878922829 1106 1 0.2375269048789761 1106 2 0.13499002683428785 1106 3 0.08616058409268135 1106 4 0.05896037086907313 1106 5 0.10209737443981481 1106 6 0.09187977630024591 1106 7 0.10634358844629332 1106 8 0.09497097470946159 1106 9 0.08125946638764958 1106 10-11 0.0841170362782222 1106 12-13 0.13126930096431266 1106 14-15 0.12531218285759138 1106 16-17 0.15146525496645324 1106 18-19 0.08597344947254726 1106 20-21 0.14019407728215327 1106 22-23 0.13451208861442154 1106 24-25 0.1686678029743618 1106 26-27 0.18752556312833235 1106 28-29 0.1370422565870797 1106 30-31 0.19283715092124964 1106 32-33 0.12975356456301057 1106 34-35 0.1968783005528607 1106 36-37 0.15334391843459372 1106 38-39 0.055262531493141864 1106 40-41 0.1764269519077999 1106 42-43 0.25423220166990745 1106 44-45 0.2118796309110138 1106 46-47 0.25675198642984043 1106 48-49 0.19559854460857196 1106 50-51 0.14347131648138145 1106 52-53 0.15030631092734126 1106 54-55 0.18477324269728967 1106 56-57 0.1747397600427476 1106 58-59 0.10172860284173879 1106 60-61 0.19742340626540766 1106 62-63 0.22688204100986553 1106 64-65 0.22379317581658853 1106 66-67 0.13790639308813013 1106 68-69 0.1205012735117279 1106 70-71 0.124031663797183 1106 72-73 0.07538836876892674 1106 74-75 0.043689673769382154 1106 76-77 0.07775857385725615 1106 78-79 0.1710079330318166 1106 80-81 0.2214687060947682 1106 82-83 0.237366899437081 1106 84-85 0.2302942220063393 1106 86-87 0.15175838990692725 1106 88-89 0.16636871118454977 1106 90-91 0.19290494985279238 1106 92-93 0.15964810983403055 1106 94-95 0.14186672417902813 1106 96-97 0.12862869854725645 1106 98-99 0.012291696831660204 1106 100 -0.05861914524400191 1107 1 0.22081695021596914 1107 2 0.15903687469133132 1107 3 0.15848266705027214 1107 4 0.10176437475541888 1107 5 0.09390141998171231 1107 6 0.12950205503891254 1107 7 0.09700767922387143 1107 8 0.09924438372079436 1107 9 0.13101735758110067 1107 10-11 0.17692348883536368 1107 12-13 0.14795682355119055 1107 14-15 0.15018555561734814 1107 16-17 0.17255013996899038 1107 18-19 0.12819976407102018 1107 20-21 0.17520072528262887 1107 22-23 0.14482935191239932 1107 24-25 0.19584163158091883 1107 26-27 0.22872639128568295 1107 28-29 0.26634544368692303 1107 30-31 0.3025178442497136 1107 32-33 0.21965775117086395 1107 34-35 0.24505328087905554 1107 36-37 0.23089450459571736 1107 38-39 0.2428053832320316 1107 40-41 0.24692773041623894 1107 42-43 0.2713607664561053 1107 44-45 0.3122624397784648 1107 46-47 0.3970785265311818 1107 48-49 0.278571050820311 1107 50-51 0.30505063033567836 1107 52-53 0.3268584586086476 1107 54-55 0.2801143509825792 1107 56-57 0.2767547346497494 1107 58-59 0.11318945169175976 1107 60-61 0.04452204097663781 1107 62-63 0.26627107794482185 1107 64-65 0.29883291746211427 1107 66-67 0.2726286012375283 1107 68-69 0.23173211618097156 1107 70-71 0.20418355456507697 1107 72-73 0.19747369059181352 1107 74-75 0.20990119727042966 1107 76-77 0.1563379113538943 1107 78-79 0.27427313702579426 1107 80-81 0.27000009467687036 1107 82-83 0.23187672620628774 1107 84-85 0.21689225271210333 1107 86-87 0.23108730212292627 1107 88-89 0.239436906947617 1107 90-91 0.09944768563164175 1107 92-93 0.08768919008626597 1107 94-95 0.19178068295354223 1107 96-97 0.24536204223818103 1107 98-99 0.2692321586678581 1107 100 0.4310477454619672 1108 1 0.27377133677558163 1108 2 0.17532779896554018 1108 3 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0.1924906882442201 2316 70-71 0.0036200056970088212 2316 72-73 -0.2202526885668945 2316 74-75 0.060570316112130484 2316 76-77 0.039996621071001215 2316 78-79 -0.1879581072996075 2316 80-81 -0.0035486531871988802 2316 82-83 0.10029947463090849 2316 84-85 0.22688947065587328 2316 86-87 0.35037415481731315 2316 88-89 0.398650606197787 2316 90-91 0.36146302009609954 2316 92-93 0.4084525661988394 2316 94-95 0.4447820880353852 2316 96-97 0.49944393172391344 2316 98-99 0.5482117083575062 2316 100 0.23065041728181157 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 7.0 11 48.0 12 86.0 13 280.0 14 832.0 15 2315.0 16 4997.0 17 9805.0 18 17090.0 19 26965.0 20 38400.0 21 52467.0 22 67128.0 23 78318.0 24 86563.0 25 96444.0 26 111983.0 27 131752.0 28 156651.0 29 185514.0 30 220874.0 31 263828.0 32 319257.0 33 397669.0 34 512487.0 35 693547.0 36 980460.0 37 1348754.0 38 1061115.0 39 122968.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.70299987177461 19.91364722641592 13.440100813270266 23.943252088539204 2 15.436674172427248 21.14496618827884 38.96622407738212 24.452135561911785 3 17.56097777359312 26.39094087434351 30.580415881029506 25.467665471033868 4 12.223282371748459 16.987830920714106 37.528897342102745 33.25998936543469 5 12.765451453130726 38.322854788807895 35.275634515006374 13.636059243055012 6 31.94209397780517 37.5078574786097 17.12684936034794 13.42319918323719 7 27.592209270679426 32.735890120702045 22.03010496788061 17.64179564073792 8 26.566299953695832 36.07429386146412 19.650380650009758 17.709025534830293 9 26.718588263710085 14.846277101582661 19.74966710137375 38.68546753333351 10-11 25.21429551596694 26.178172814944116 28.421520084789552 20.186011584299397 12-13 25.469734784971575 23.81248785507886 28.665626961252727 22.052150398696835 14-15 22.70506484656289 25.199766047981626 25.94552924489661 26.14963986055887 16-17 22.516036808464058 28.31898617893973 26.582310359284605 22.582666653311602 18-19 22.43568215656071 27.287135286580096 28.54058826349156 21.736594293367638 20-21 23.080620904885304 26.483678208945825 28.61030462859751 21.825396257571356 22-23 22.910096753103943 26.344984333801285 28.463337431905302 22.281581481189473 24-25 22.684795496901707 26.54821727437808 28.531873396006258 22.235113832713957 26-27 22.705546444690018 26.64466703860557 28.471455597062818 22.178330919641592 28-29 22.68191830743505 26.538986797441623 28.6323634247951 22.14673147032823 30-31 22.84298937158845 26.53949128176627 28.571261970851914 22.046257375793367 32-33 22.64658577070574 26.691345658519833 28.50514423902467 22.156924331749757 34-35 22.85402950347222 26.694532170060658 28.47880681078312 21.972631515684004 36-37 22.946848981369683 26.635472094019576 28.319423819296325 22.098255105314415 38-39 22.836527918675674 26.728176573982065 28.320204578621457 22.115090928720804 40-41 22.79819415767163 26.69903739256722 28.39112378897763 22.111644660783515 42-43 22.727168649180854 26.711356715170524 28.27820360172645 22.283271033922173 44-45 22.738315579732646 26.878495423483407 28.139216200877325 22.243972795906622 46-47 22.77665787518887 26.863163898837 27.96739564240155 22.392782583572583 48-49 22.82055758477714 26.952029678707245 27.86106529959539 22.366347436920226 50-51 22.751462251694104 27.15056300330246 27.678926167889223 22.41904857711421 52-53 22.79358030464619 27.16274713026745 27.57727876132471 22.46639380376165 54-55 22.753015998033266 27.14516285516484 27.621756798641833 22.480064348160063 56-57 22.764682406297226 27.203757912994302 27.509924736135744 22.521634944572728 58-59 22.760305385893147 27.109691184886763 27.629611891416282 22.500391537803807 60-61 22.84948610046395 27.230543504408033 27.593470786296415 22.326499608831604 62-63 22.657282483027345 27.26528319054039 27.658028777635263 22.419405548797005 64-65 22.643163718208747 27.090045147177484 27.651267085275638 22.61552404933813 66-67 22.61458449528129 27.174209588591896 27.53559612285797 22.675609793268844 68-69 22.586930687288234 27.156573410521094 27.605215841237296 22.651280060953376 70-71 22.565469674529755 27.06279736990849 27.566578663060664 22.80515429250109 72-73 22.605114971697223 27.252331815651736 27.354432043600923 22.788121169050115 74-75 22.551369903444666 27.30441636530167 27.334932679151336 22.80928105210233 76-77 22.526646734263593 27.253292152038426 27.452081955432277 22.767979158265703 78-79 22.491949435521317 27.23128345278501 27.406967259714005 22.869799851979668 80-81 22.443585179817365 27.42650911987242 27.248550617001992 22.881355083308218 82-83 22.527814577023655 27.342770700094583 27.303687253429747 22.825727469452016 84-85 22.47344353799914 27.3678044940604 27.251874910548164 22.9068770573923 86-87 22.387616588752998 27.30949791138986 27.373633487543508 22.92925201231363 88-89 22.32752558788065 27.259685870692767 27.38618252655997 23.026606014866612 90-91 22.431255792809935 27.365250232571185 27.24528436376617 22.958209610852705 92-93 22.39014385237018 27.275466780077455 27.252352390110218 23.082036977442144 94-95 22.22456160640216 27.16780760548269 27.37557545511121 23.23205533300394 96-97 22.267906030589483 27.20808300481946 27.32828505704159 23.195725907549466 98-99 22.246806066125497 27.131044187877208 27.271439244683183 23.35071050131411 100 21.210165722957743 27.67359864806613 27.531741765517097 23.58449386345903 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1656.0 1 1304.0 2 1829.0 3 2697.5 4 2017.5 5 1639.5 6 2707.5 7 5200.0 8 8258.5 9 9801.5 10 8439.0 11 6764.0 12 6889.5 13 7402.5 14 7839.0 15 8210.0 16 8692.5 17 9176.0 18 9854.0 19 10571.5 20 11459.5 21 13020.0 22 15431.0 23 18807.0 24 23318.5 25 29132.0 26 36137.5 27 44128.0 28 52771.0 29 63087.5 30 75628.5 31 87958.5 32 99760.5 33 113456.5 34 128922.5 35 142907.5 36 159698.5 37 177086.5 38 189412.5 39 199908.5 40 208209.0 41 215192.0 42 222535.5 43 231438.5 44 239901.0 45 248698.5 46 256276.5 47 260412.0 48 265671.5 49 269298.0 50 269836.5 51 265070.0 52 255947.5 53 248312.5 54 238644.0 55 223269.0 56 204722.0 57 184667.5 58 164533.5 59 144031.0 60 121884.5 61 100879.5 62 82243.0 63 64595.5 64 48519.0 65 36315.5 66 27471.0 67 20909.0 68 15889.0 69 12111.5 70 9424.0 71 7278.5 72 5614.0 73 4505.0 74 3498.0 75 2551.5 76 1809.5 77 1259.5 78 879.5 79 603.5 80 419.5 81 294.0 82 225.0 83 173.0 84 134.0 85 97.5 86 66.0 87 49.5 88 34.0 89 17.5 90 13.5 91 11.0 92 7.0 93 7.0 94 4.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23632726031227366 2 0.0728184444011359 3 0.0017886236097744129 4 0.001173337088012015 5 6.868314661533746E-4 6 7.0114045503157E-4 7 0.0 8 6.009775328842027E-4 9 0.0 10-11 2.432528109293202E-4 12-13 0.0013736629323067493 14-15 2.1463483317292957E-5 16-17 5.723595551278121E-5 18-19 0.001130410121377429 20-21 0.0014881348433323117 22-23 1.4308988878195303E-5 24-25 5.0081461073683564E-5 26-27 1.5739887766014833E-4 28-29 0.0017170786653834364 30-31 0.0018387050708480964 32-33 0.003062123619933795 34-35 0.004063752841407467 36-37 0.001602606754357874 38-39 0.0028689522700781588 40-41 0.005823758473425489 42-43 0.01221987650197879 44-45 0.007841325905251027 46-47 0.005823758473425489 48-49 0.0062816461175277385 50-51 0.0047720477908781336 52-53 0.0035057022751578495 54-55 0.005437415773714216 56-57 0.0038205000304781466 58-59 0.0020676488928992216 60-61 0.008227668604962301 62-63 0.010831904580793847 64-65 0.006718070278312695 66-67 0.007562300622126219 68-69 0.013994191122875007 70-71 0.003777573063843561 72-73 0.012484592796225403 74-75 0.007848480399690124 76-77 0.00469334835204806 78-79 0.01517468270532612 80-81 0.006696606794995402 82-83 0.0143232978670735 84-85 0.023094708049407223 86-87 0.015346390571864465 88-89 0.01541793551625544 90-91 0.029240418772592102 92-93 0.023244952432628273 94-95 0.02818870809004475 96-97 0.017349649014811806 98-99 0.01700623328173512 100 0.018458595652871942 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6988614.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.40664581663547 #Duplication Level Percentage of deduplicated Percentage of total 1 70.93042849041072 39.300171289903766 2 16.569442192245347 18.36114429849908 3 5.776603481725668 9.601866694053522 4 2.499115632134952 5.538704587380734 5 1.3037499942195807 3.611820708158242 6 0.7633755434520729 2.5377647016678373 7 0.4797657496970141 1.860754767789053 8 0.3246466919754189 1.439006742225951 9 0.23565183234144244 1.1751009849525118 >10 1.039197920320737 9.804772595902488 >50 0.04978525209949725 1.8895800756816197 >100 0.02617883923676211 2.545027617145602 >500 0.0014484903746265652 0.5310833248384575 >1k 4.955354348492634E-4 0.5584963916486058 >5k 0.0 0.0 >10k+ 1.1435433111840882E-4 1.2447052201525273 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23652 0.3384362049470754 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20778 0.29731217091114204 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14505 0.20755188367822286 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 1.4308988878195305E-5 7 0.0 0.0 0.0 0.0 1.4308988878195305E-5 8 0.0 0.0 0.0 0.0 5.723595551278122E-5 9 0.0 0.0 0.0 0.0 7.154494439097652E-5 10-11 0.0 0.0 0.0 0.0 7.154494439097652E-5 12-13 0.0 0.0 0.0 0.0 7.154494439097652E-5 14-15 0.0 0.0 0.0 0.0 7.154494439097652E-5 16-17 0.0 0.0 0.0 0.0 1.1447191102556244E-4 18-19 0.0 0.0 0.0 0.0 1.1447191102556244E-4 20-21 0.0 0.0 0.0 1.4308988878195305E-5 1.1447191102556244E-4 22-23 0.0 0.0 0.0 2.1463483317292957E-5 1.359353943428554E-4 24-25 0.0 0.0 0.0 8.585393326917182E-5 1.7170786653834366E-4 26-27 0.0 0.0 0.0 1.931713498556366E-4 1.7170786653834366E-4 28-29 0.0 0.0 0.0 9.515477603999877E-4 1.7170786653834366E-4 30-31 0.0 0.0 0.0 0.004178224752433029 1.7170786653834366E-4 32-33 0.0 0.0 0.0 0.01011645513688408 1.8601685541653896E-4 34-35 0.0 0.0 0.0 0.01892363779141329 2.0032584429473425E-4 36-37 0.0 0.0 0.0 0.03427718285771685 2.0032584429473425E-4 38-39 0.0 0.0 0.0 0.05702847517404738 2.0032584429473425E-4 40-41 0.0 0.0 0.0 0.08112481244492828 2.0032584429473425E-4 42-43 0.0 0.0 0.0 0.10477041656614602 2.074803387338319E-4 44-45 0.0 0.0 0.0 0.13327392241151106 2.1463483317292958E-4 46-47 0.0 0.0 0.0 0.16918948449578128 2.2894382205112488E-4 48-49 0.0 0.0 0.0 0.20690082468426502 2.2894382205112488E-4 50-51 0.0 0.0 0.0 0.24724501882633665 2.2894382205112488E-4 52-53 0.0 0.0 0.0 0.28632286745268803 2.9333427200300375E-4 54-55 0.0 0.0 0.0 0.3289851177930273 3.6487921639398024E-4 56-57 0.0 0.0 0.0 0.37943861257754397 3.720337108330779E-4 58-59 0.0 0.0 0.0 0.43764757933404247 3.720337108330779E-4 60-61 0.0 0.0 0.0 0.49655768654557253 3.863426997112732E-4 62-63 0.0 0.0 0.0 0.5547451898187538 4.006516885894685E-4 64-65 0.0 0.0 0.0 0.6137697689413094 4.149606774676638E-4 66-67 0.0 0.0 0.0 0.6801634773361356 4.2926966634585916E-4 68-69 0.0 0.0 0.0 0.7576752700893196 4.2926966634585916E-4 70-71 0.0 0.0 0.0 0.8415688718821785 4.7219663298044505E-4 72-73 0.0 0.0 0.0 0.9228439287103279 6.009775328842027E-4 74-75 0.0 0.0 0.0 1.0058146007205435 6.009775328842027E-4 76-77 0.0 0.0 0.0 1.0966981435804009 6.009775328842027E-4 78-79 0.0 0.0 0.0 1.1966965123556688 6.009775328842027E-4 80-81 0.0 0.0 0.0 1.3035417323091532 6.009775328842027E-4 82-83 0.0 0.0 0.0 1.4157528230919607 6.439044995187887E-4 84-85 0.0 0.0 0.0 1.5247730093549308 6.439044995187887E-4 86-87 0.0 0.0 0.0 1.6385366254310225 6.439044995187887E-4 88 0.0 0.0 0.0 1.7302572441402544 6.725224772751793E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12130 0.0 44.969837 1 GTATCAA 19615 0.0 35.765423 1 TCAACGC 24525 0.0 28.184162 4 CAACGCA 24655 0.0 27.997639 5 ATCAACG 24905 0.0 27.773396 3 GTACATG 25275 0.0 27.51374 1 AACGCAG 25495 0.0 27.075958 6 TATCAAC 26360 0.0 26.423124 2 TACATGG 26315 0.0 26.218102 2 ACATGGG 26420 0.0 25.238136 3 CATGGGG 16865 0.0 24.072926 4 TACCTGG 5600 0.0 23.598957 2 ACGCAGA 29340 0.0 23.287252 7 CGCAGAG 30160 0.0 22.654108 8 GTACCTG 6095 0.0 22.12285 1 ATGGGGG 8965 0.0 20.913523 5 GCAGAGT 34405 0.0 19.694935 9 AGAGTAC 31060 0.0 19.372644 10-11 GAGTACT 20580 0.0 19.15686 12-13 TAACGCA 695 0.0 18.93105 4 >>END_MODULE