FastQCFastQC Report
Mon 6 Jun 2016
ERR1211565_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211565_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3604494
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124070.3442092010695537No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102770.2851163020385108No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70210.19478462164176166No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42990.1192677807204007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54750.047.8946571
TACCTGG36800.039.762142
GTATCAA94800.039.7000431
GTACCTG39000.038.8183331
ATAACGC4700.032.9918253
TAACGCA4600.032.6857384
TCAACGC114500.031.8846534
ATCAACG115450.031.6647383
ACCTGGG45200.031.602793
CAACGCA115950.031.5229455
TATAACG4950.030.4159932
TATCAAC122150.030.1210752
AACGCAG123650.029.7466056
GTACATG107900.027.7117671
TACATGG110550.026.5998062
ACGCAGA138100.026.5664257
ACATGGG109000.026.0806943
CGCAGAG142550.025.8384698
CATGGGG70900.023.9899024
GCAGAGT158050.022.4127319
Produced by FastQC (version 0.11.5)