FastQCFastQC Report
Mon 6 Jun 2016
ERR1211565_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211565_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3604494
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147070.4080184347650461No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128420.3562774691815273No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92700.25717895493791915No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46930.1301985798838894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62600.052.268631
GTACCTG41400.042.9335671
TACCTGG41900.041.979082
GTATCAA108550.039.090271
ACCTGGG47400.035.5911373
TCAACGC140600.030.0456814
ATCAACG141050.029.9177533
CAACGCA142200.029.7080255
TATCAAC146450.028.9341532
AACGCAG148550.028.6599396
GTACATG113400.027.0659891
CCTGGGG46650.025.9881734
TACATGG113600.025.87482
TATAACG4050.025.5471822
ACGCAGA166100.025.5185897
ACATGGG114800.025.1334573
CGCAGAG170550.024.7700948
ATAACGC4450.024.2881033
CATGGGG78850.023.2418464
TAACGCA4050.023.2050574
Produced by FastQC (version 0.11.5)