FastQCFastQC Report
Mon 6 Jun 2016
ERR1211564_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211564_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5120082
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146670.28646025590996393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123910.24200784284314197No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80730.15767325601425916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78000.048.6795431
GTATCAA133700.037.5366441
TCAACGC159700.030.6584634
ATCAACG160100.030.5516173
CAACGCA160850.030.4085715
GTACCTG43800.029.2913931
TACCTGG42900.029.064442
AACGCAG168950.029.0307166
TATCAAC171900.028.7400042
GTACATG181300.028.5400071
TACATGG186900.027.4907592
ACATGGG184450.026.6712173
ACGCAGA192550.025.3017567
CATGGGG114600.024.5190754
CGCAGAG198650.024.289028
ACCTGGG56250.021.8853533
GCAGAGT220900.021.1619439
ATGGGGG68350.020.3478975
GAGTACT133400.019.63687512-13
GAGTACA133850.019.4168361
Produced by FastQC (version 0.11.5)