FastQCFastQC Report
Mon 6 Jun 2016
ERR1211564_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211564_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5120082
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176140.3440179278378745No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152320.2974952354278701No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105120.20530921184465406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81750.050.0065961
GTATCAA143400.038.503811
TCAACGC177950.030.1292364
ATCAACG179200.029.9726873
CAACGCA180800.029.65435
GTACATG181000.029.6455571
AACGCAG188400.028.5836016
TATCAAC190600.028.4509832
TACATGG186250.028.1811522
TACCTGG42550.027.4121422
ACATGGG186300.027.3170033
GTACCTG44600.026.6416821
CATGGGG116400.025.513184
ACGCAGA217400.024.554317
CGCAGAG223050.023.9323358
ATGGGGG66250.021.9875225
GCAGAGT251000.020.8927299
TATAACG5050.020.4890652
AGAGTAC227800.020.15327310-11
GAGTACT155300.019.75806212-13
Produced by FastQC (version 0.11.5)