FastQCFastQC Report
Mon 6 Jun 2016
ERR1211563_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211563_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2955055
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97260.3291309298811697No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84390.2855784410104042No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56590.19150235782413524No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32260.10916886487730346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46350.047.3138661
TACCTGG34750.046.4313932
GTACCTG37350.043.8151251
GTATCAA75350.035.801231
ACCTGGG44800.035.556253
TATAACG3800.032.1856422
TAACGCA3300.031.3263934
ATAACGC3850.029.2917143
TCAACGC92800.028.6089654
ATCAACG93350.028.4399223
CAACGCA93950.028.3549655
TATCAAC97650.027.5547332
AACGCAG98050.027.308426
GTACATG94950.026.672551
TACATGG95400.025.6405542
ACATGGG94350.024.50293
CCTGGGG42600.024.4875324
GTATAAC8550.023.7185171
ACGCAGA112700.023.592247
CATGGGG63650.022.885744
Produced by FastQC (version 0.11.5)