FastQCFastQC Report
Mon 6 Jun 2016
ERR1211563_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211563_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2955055
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116940.3957286751008019No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103310.34960432208537573No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75160.2543438277798552No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35810.12118217765828385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55150.047.9445151
TACCTGG36450.044.899982
GTACCTG37750.044.073681
TAACGCA3700.038.1005334
ACCTGGG42700.037.8566443
GTATCAA92450.037.522561
TATAACG4050.034.836192
ATAACGC4700.030.9938413
CCTGGGG37750.029.62594
ATCAACG118050.029.0581823
TCAACGC118550.029.0148984
CAACGCA119950.028.676255
TATCAAC120850.028.3690812
AACGCAG126850.027.2655076
GTACATG104250.025.6799321
TACATGG107100.024.7219852
CATGGGG72850.023.995234
ACGCAGA144600.023.6261187
ACATGGG106700.023.517353
GTATAAC9100.023.3073371
Produced by FastQC (version 0.11.5)