##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211563_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2955055 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.79341467417696 31.0 31.0 34.0 30.0 34.0 2 32.082152447247175 33.0 31.0 34.0 30.0 34.0 3 32.16535259073012 33.0 31.0 34.0 30.0 34.0 4 35.875430744943834 37.0 35.0 37.0 35.0 37.0 5 35.75186993135492 37.0 35.0 37.0 35.0 37.0 6 35.78396882629934 37.0 35.0 37.0 35.0 37.0 7 35.6773058369472 37.0 35.0 37.0 33.0 37.0 8 35.653825055709625 37.0 35.0 37.0 33.0 37.0 9 37.24475280493933 39.0 37.0 39.0 34.0 39.0 10-11 37.18600195258633 39.0 37.0 39.0 33.0 39.0 12-13 37.079331179961116 39.0 37.0 39.0 33.0 39.0 14-15 38.21084616022375 40.0 38.0 41.0 33.0 41.0 16-17 38.12219535677001 40.0 37.0 41.0 33.0 41.0 18-19 38.10599092064276 40.0 37.0 41.0 33.0 41.0 20-21 38.02815260629667 40.0 37.0 41.0 33.0 41.0 22-23 37.90670292092702 40.0 37.0 41.0 33.0 41.0 24-25 37.772173445164306 39.5 37.0 41.0 32.5 41.0 26-27 37.534986996857924 39.0 37.0 41.0 32.0 41.0 28-29 37.53891365812142 39.0 37.0 41.0 32.0 41.0 30-31 37.39973029266798 39.0 36.5 41.0 32.0 41.0 32-33 37.18552920334816 39.0 36.0 40.5 31.0 41.0 34-35 37.01758038344464 39.0 36.0 40.0 31.0 41.0 36-37 36.90602814499222 39.0 36.0 40.0 31.0 41.0 38-39 36.81581933331191 39.0 35.0 40.0 30.0 41.0 40-41 36.628848194026844 39.0 35.0 40.0 30.0 41.0 42-43 36.41759950322414 39.0 35.0 40.0 30.0 41.0 44-45 36.18751969083486 38.0 35.0 40.0 29.0 41.0 46-47 35.90496826624208 38.0 35.0 40.0 28.0 41.0 48-49 35.61934617122185 38.0 34.0 40.0 27.0 41.0 50-51 35.38292468329693 38.0 34.0 40.0 26.0 41.0 52-53 35.14486853882585 38.0 34.0 40.0 26.0 41.0 54-55 34.896112931908206 38.0 33.0 40.0 25.5 41.0 56-57 34.65512249348997 37.5 33.0 40.0 25.0 41.0 58-59 34.35316432350667 37.0 33.0 40.0 24.0 41.0 60-61 33.99931744079214 36.5 32.0 39.5 23.0 41.0 62-63 33.61584319073587 36.0 31.5 39.0 22.5 40.0 64-65 33.264078671970566 36.0 31.0 39.0 22.0 40.0 66-67 32.86810719259032 35.0 31.0 38.5 22.0 40.0 68-69 32.45203947134656 35.0 30.5 38.0 20.5 40.0 70-71 32.063769540668446 35.0 30.0 37.5 20.0 39.5 72-73 31.619376627507776 34.0 29.5 37.0 20.0 39.0 74-75 31.213433252511376 34.0 29.0 36.0 19.0 39.0 76-77 29.84112986052713 32.5 28.0 35.0 18.5 37.0 78-79 30.751129166800617 34.0 29.0 35.0 19.0 37.5 80-81 30.73139890797295 34.0 29.0 35.0 19.0 37.0 82-83 30.44802804008724 34.0 29.0 35.0 18.0 37.0 84-85 30.117438930916684 34.0 29.0 35.0 18.0 36.0 86-87 29.802748510603017 33.5 29.0 35.0 17.5 36.0 88-89 29.50114955559203 33.0 27.5 35.0 16.5 35.5 90-91 29.19449570312566 33.0 27.0 35.0 15.0 35.0 92-93 28.93090619294734 33.0 26.5 35.0 12.5 35.0 94-95 28.676260509533662 32.5 26.0 34.5 11.5 35.0 96-97 28.412836816912037 32.0 25.0 34.0 10.5 35.0 98-99 28.17076653395622 32.0 25.0 34.0 10.0 35.0 100 26.3274084577106 30.0 20.0 34.0 8.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13432963624210004 1101 2 -0.0012614625435745097 1101 3 -0.00192582375628092 1101 4 0.05551535722264589 1101 5 -0.011706027424153831 1101 6 0.03898110317604875 1101 7 0.06541213605351714 1101 8 0.002307875149419658 1101 9 -0.045599137902762266 1101 10-11 0.007973715361210054 1101 12-13 -0.025297602619353654 1101 14-15 -0.12620103654071357 1101 16-17 -0.10300236194130008 1101 18-19 -0.0714088276304281 1101 20-21 -0.12234552878754101 1101 22-23 -0.20500359875077123 1101 24-25 -0.2920874514002847 1101 26-27 -0.38192900116336403 1101 28-29 -0.13387304941998224 1101 30-31 -0.0861991916569309 1101 32-33 -0.04473951248682084 1101 34-35 -0.26953810853905935 1101 36-37 -0.29689976641434157 1101 38-39 -0.46461879648663285 1101 40-41 -0.3447705776807979 1101 42-43 -0.32867288932319383 1101 44-45 -0.2862003675493483 1101 46-47 -0.300014968691805 1101 48-49 -0.43372977737765694 1101 50-51 -0.5479468050455338 1101 52-53 -0.5395380181350546 1101 54-55 -0.6276208485210901 1101 56-57 -0.6167528631293777 1101 58-59 -0.9298354215902478 1101 60-61 -0.7418145349693077 1101 62-63 -0.8910344841943072 1101 64-65 -0.9082889878706268 1101 66-67 -0.9083567323207227 1101 68-69 -0.9103066714551957 1101 70-71 -1.1237063165649381 1101 72-73 -1.2803653358461418 1101 74-75 -1.1218466899045652 1101 76-77 -0.8755802238926442 1101 78-79 -0.7362895574414736 1101 80-81 -0.7280710295954123 1101 82-83 -0.9046940273957276 1101 84-85 -1.0133067991374638 1101 86-87 -1.1394041529789547 1101 88-89 -1.3235090966314615 1101 90-91 -1.4184469493831884 1101 92-93 -1.3033175687256886 1101 94-95 -1.6384090964625315 1101 96-97 -1.649316800591997 1101 98-99 -1.5438526973507543 1101 100 -1.7843309603544135 1102 1 0.18833631796617212 1102 2 0.19081457735855167 1102 3 0.11293259680866186 1102 4 0.1203703391971942 1102 5 0.03542392595579713 1102 6 0.05663238305559304 1102 7 0.08852534530802814 1102 8 0.010124786498934668 1102 9 0.02390879981170002 1102 10-11 0.1553555454876019 1102 12-13 0.21199182896356206 1102 14-15 0.1902302676623293 1102 16-17 0.13879433773583827 1102 18-19 0.08018957396797077 1102 20-21 -0.04208249472015524 1102 22-23 0.08931010385097693 1102 24-25 0.07350144340057341 1102 26-27 0.05696702129243647 1102 28-29 0.09751965208140945 1102 30-31 0.054203204859597065 1102 32-33 0.12383330961860395 1102 34-35 0.07651212745683011 1102 36-37 0.022929860480502384 1102 38-39 0.1291370853127276 1102 40-41 0.04736084162453125 1102 42-43 -0.023618794866919757 1102 44-45 -0.016489779224635015 1102 46-47 -0.157850294597786 1102 48-49 -0.232706389533611 1102 50-51 -0.06129532247013714 1102 52-53 -0.182677034915379 1102 54-55 -0.18333845518978364 1102 56-57 -0.1828043388275944 1102 58-59 -0.16591772696602902 1102 60-61 -0.14858846755846145 1102 62-63 -0.21640137274054894 1102 64-65 -0.14781395565646704 1102 66-67 -0.4500107304964587 1102 68-69 -0.3701541156776429 1102 70-71 -0.4470401385400642 1102 72-73 -0.4623355395554718 1102 74-75 -0.5443947034851853 1102 76-77 -0.3497007591544836 1102 78-79 -0.3311575517659904 1102 80-81 -0.26420699507920276 1102 82-83 -0.3664750000462682 1102 84-85 -0.39495572086790887 1102 86-87 -0.4753793191171667 1102 88-89 -0.48477649770864417 1102 90-91 -0.584170265921724 1102 92-93 -0.6858609764049639 1102 94-95 -0.6402767939824052 1102 96-97 -0.6370622978483667 1102 98-99 -0.809290158440394 1102 100 -0.9077501136648713 1103 1 0.13100205642652085 1103 2 0.09208453625166158 1103 3 0.059183062087029725 1103 4 0.040034659279655216 1103 5 0.12797201469265929 1103 6 0.0963188655596312 1103 7 0.035163952109662944 1103 8 0.019274477716493266 1103 9 0.026388931196478893 1103 10-11 0.04986358331841956 1103 12-13 0.11064228663509112 1103 14-15 0.15269758065461048 1103 16-17 0.030005149117215524 1103 18-19 0.06201070450788393 1103 20-21 0.086799668376635 1103 22-23 -0.013708137198051418 1103 24-25 0.07986427207086422 1103 26-27 0.034399364154417356 1103 28-29 0.11152421063426488 1103 30-31 0.22961240410216277 1103 32-33 0.27956518662528396 1103 34-35 0.11046083551072883 1103 36-37 0.19893056689166144 1103 38-39 0.14291792563004435 1103 40-41 0.142889062387205 1103 42-43 0.07679116292361954 1103 44-45 0.06969478639660309 1103 46-47 0.10618407168559685 1103 48-49 0.16669214602006122 1103 50-51 0.09819681576764339 1103 52-53 0.14907440536424588 1103 54-55 0.07075727655687558 1103 56-57 -0.07401333396733634 1103 58-59 0.02636547895260577 1103 60-61 -0.06759333166725412 1103 62-63 -0.02861170890764697 1103 64-65 -0.1599704883357802 1103 66-67 -0.16811870242975147 1103 68-69 -0.10790709142931831 1103 70-71 -0.05997805042627391 1103 72-73 -0.1274205268439168 1103 74-75 -0.1837845189422147 1103 76-77 -0.12111772037280844 1103 78-79 0.061832588819658696 1103 80-81 -0.0803297828434637 1103 82-83 -0.20533820158028604 1103 84-85 -0.26406885434628435 1103 86-87 -0.16179285939093546 1103 88-89 -0.30576072332944904 1103 90-91 -0.3691803001226184 1103 92-93 -0.47017223834248156 1103 94-95 -0.178219310575372 1103 96-97 -0.30213585349822125 1103 98-99 -0.4497621868233317 1103 100 -0.4101269329513926 1104 1 0.26833277974170855 1104 2 0.12872339358969498 1104 3 0.11684442984170573 1104 4 0.07299886752859663 1104 5 0.09868016324030293 1104 6 0.14305933501488255 1104 7 0.1883707807656947 1104 8 0.17541689951727335 1104 9 0.17930901489850015 1104 10-11 0.17267247728832302 1104 12-13 0.21342050347949737 1104 14-15 0.1946187134972277 1104 16-17 0.16433531363401244 1104 18-19 0.2073980545333427 1104 20-21 0.12315433354094552 1104 22-23 0.043260228852723515 1104 24-25 -0.008259475048014053 1104 26-27 -0.013658965104013987 1104 28-29 0.024965682519457744 1104 30-31 0.05663340639872416 1104 32-33 0.04521547510508839 1104 34-35 0.11699861004272094 1104 36-37 0.16001103479857903 1104 38-39 0.03743480362350482 1104 40-41 0.03912865029969481 1104 42-43 -0.013739699721270426 1104 44-45 0.07480164942661105 1104 46-47 0.18907086817450391 1104 48-49 0.07681318010655502 1104 50-51 -0.16042321997336018 1104 52-53 -0.1279422155277672 1104 54-55 -0.22578201818181753 1104 56-57 -0.11446015804294518 1104 58-59 -0.02016790271336788 1104 60-61 -0.10786687926812277 1104 62-63 -0.14666925357687433 1104 64-65 -0.19038321872810826 1104 66-67 -0.24084579802533312 1104 68-69 -0.3253688067580569 1104 70-71 -0.16157933135933078 1104 72-73 -0.16694209830629347 1104 74-75 -0.4032835613370196 1104 76-77 -0.23658174445688473 1104 78-79 -0.14190095134792102 1104 80-81 0.08718350385700191 1104 82-83 -0.0990451709518183 1104 84-85 -0.25887665551783456 1104 86-87 -0.2467081784587748 1104 88-89 -0.3853599555781706 1104 90-91 -0.2259628654459256 1104 92-93 -0.3479023456978325 1104 94-95 -0.3216009046543391 1104 96-97 -0.39770897820237394 1104 98-99 -0.4745258228118665 1104 100 -0.5144217617791362 1105 1 0.2697177136482516 1105 2 0.1887883118435525 1105 3 0.15218210633056373 1105 4 0.17710824048721463 1105 5 0.09784313596536975 1105 6 0.027806578823813766 1105 7 0.08023945095613527 1105 8 0.05215349314262596 1105 9 0.12967252343817393 1105 10-11 0.15711801680219395 1105 12-13 0.13920645608651938 1105 14-15 0.19965290640659816 1105 16-17 0.22350305831412243 1105 18-19 0.22969669335461163 1105 20-21 0.14975348502834862 1105 22-23 0.13466795872952275 1105 24-25 0.14373001284339182 1105 26-27 0.1637195307426964 1105 28-29 0.1321307489633341 1105 30-31 0.22567018591274746 1105 32-33 0.22564537574444898 1105 34-35 0.17957811429705117 1105 36-37 0.3264338561662683 1105 38-39 0.09054006288909733 1105 40-41 0.22537114155730364 1105 42-43 0.17579117274628686 1105 44-45 0.21757525990850723 1105 46-47 0.12200607136387731 1105 48-49 0.22527804258648843 1105 50-51 0.2775956006926563 1105 52-53 0.14081686590959208 1105 54-55 0.20125246698626142 1105 56-57 0.26938112560250005 1105 58-59 0.2239048364421805 1105 60-61 0.15505356558854544 1105 62-63 0.14481885267558425 1105 64-65 0.26134827815217676 1105 66-67 0.19705869237181162 1105 68-69 0.0489744871859088 1105 70-71 0.18300922245482099 1105 72-73 0.051157220074006915 1105 74-75 0.10746269941988373 1105 76-77 0.12120588848081937 1105 78-79 0.29019110533436887 1105 80-81 0.22941051523552858 1105 82-83 0.3574243898431675 1105 84-85 0.2089805347839082 1105 86-87 0.17511256402270092 1105 88-89 0.21391266123457342 1105 90-91 0.23787461695343737 1105 92-93 -0.03964487602437927 1105 94-95 -0.09093869808852162 1105 96-97 0.09702000937432942 1105 98-99 0.2023171617709103 1105 100 0.23828333108517796 1106 1 0.2487181927118094 1106 2 0.18745394508544422 1106 3 0.11639842398550115 1106 4 0.0788580158728962 1106 5 0.12521251868941619 1106 6 0.08219819367717207 1106 7 0.04038660530154914 1106 8 0.17632152511202293 1106 9 0.12428284529728018 1106 10-11 0.11022887185392705 1106 12-13 0.13518295085858512 1106 14-15 0.2271247933885192 1106 16-17 0.1868759534605715 1106 18-19 0.21398003089324646 1106 20-21 0.2523739925288453 1106 22-23 0.12814125050991976 1106 24-25 0.1514599207302041 1106 26-27 0.20928320613195694 1106 28-29 0.2512529502348073 1106 30-31 0.3019734178016691 1106 32-33 0.16018612470320903 1106 34-35 0.2352426146644504 1106 36-37 0.15308333294703402 1106 38-39 0.09287933365547474 1106 40-41 0.15836862604741242 1106 42-43 0.15236063866714034 1106 44-45 0.22788919145185815 1106 46-47 0.17099953709037408 1106 48-49 0.3350703851561505 1106 50-51 0.29215157824656757 1106 52-53 0.19032684867352856 1106 54-55 0.10014672095132227 1106 56-57 0.2092883240865504 1106 58-59 0.22427862535757015 1106 60-61 0.1320743379378868 1106 62-63 0.09225276193652121 1106 64-65 0.18360194188024792 1106 66-67 0.20570639695837656 1106 68-69 0.09481296349101598 1106 70-71 0.10244220768567658 1106 72-73 0.09727763636489684 1106 74-75 0.002838670546502442 1106 76-77 0.15257192500557792 1106 78-79 0.29494785697656667 1106 80-81 0.12753279066809142 1106 82-83 0.22792384556900203 1106 84-85 0.3028169494961013 1106 86-87 0.18704786359086256 1106 88-89 0.3510825911310391 1106 90-91 0.20079604584148214 1106 92-93 0.2441885083124582 1106 94-95 0.3522002614570354 1106 96-97 0.11812501806055309 1106 98-99 0.25788842801602385 1106 100 0.15764231506127047 1107 1 0.17762607622384152 1107 2 0.13497769765724854 1107 3 0.13005092553276398 1107 4 0.11067391671426918 1107 5 0.1257279244067604 1107 6 0.17219367734309543 1107 7 0.07030606401054484 1107 8 0.10642414625849739 1107 9 0.04476902029686869 1107 10-11 0.12485604429425479 1107 12-13 0.153870014906893 1107 14-15 0.1439278561321018 1107 16-17 0.20755020450184247 1107 18-19 0.10188576914659819 1107 20-21 0.11596460357211669 1107 22-23 0.0652811983211663 1107 24-25 0.09338425540728679 1107 26-27 0.04525706157581055 1107 28-29 0.16376633196539814 1107 30-31 0.14847576796811524 1107 32-33 0.07598754400300578 1107 34-35 0.13029095616620623 1107 36-37 0.13593955879719033 1107 38-39 0.15788234454485917 1107 40-41 0.1470499819512625 1107 42-43 0.19734855329170387 1107 44-45 0.15824313822602676 1107 46-47 0.19839120779943897 1107 48-49 0.22262631660823473 1107 50-51 -0.02218077794164941 1107 52-53 0.13396174488148205 1107 54-55 0.1691389511839958 1107 56-57 0.14444976212076455 1107 58-59 0.08555775153055833 1107 60-61 0.0017041867243321462 1107 62-63 0.23213251454177453 1107 64-65 0.24453199306537243 1107 66-67 0.2746366321422897 1107 68-69 0.23272612442304563 1107 70-71 0.020571629929463597 1107 72-73 0.021397996667992913 1107 74-75 0.15177858657614252 1107 76-77 0.038300204784111 1107 78-79 0.13471376032701698 1107 80-81 0.14850447158072555 1107 82-83 0.0884992808934264 1107 84-85 0.07534728838842852 1107 86-87 0.05020346384511498 1107 88-89 0.06340601501408116 1107 90-91 0.008841915904877595 1107 92-93 -0.08188757100775135 1107 94-95 0.017885008793236068 1107 96-97 0.015383854184534584 1107 98-99 0.11558187765890082 1107 100 0.09395740463522984 1108 1 0.22007505168244634 1108 2 0.10258730358728485 1108 3 0.12777247912941903 1108 4 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2316 82-83 0.021899867908459214 2316 84-85 0.08925723070181846 2316 86-87 0.40788428761475615 2316 88-89 0.45062950698220305 2316 90-91 0.33342804188983166 2316 92-93 0.3603889796496951 2316 94-95 0.5636797930469406 2316 96-97 0.5378022668081996 2316 98-99 0.45016583176942504 2316 100 0.2647085484214351 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 1.0 10 5.0 11 19.0 12 53.0 13 160.0 14 468.0 15 1204.0 16 2586.0 17 5337.0 18 9373.0 19 14225.0 20 19242.0 21 25572.0 22 32581.0 23 37219.0 24 40731.0 25 45476.0 26 51683.0 27 60869.0 28 72945.0 29 85914.0 30 101295.0 31 120602.0 32 146096.0 33 180109.0 34 231814.0 35 308172.0 36 420614.0 37 537912.0 38 368419.0 39 34358.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.73841342631536 19.897328575692615 13.693322117602365 23.670935880389656 2 15.4580510054423 21.094156104733557 39.25314064204317 24.194652247780972 3 17.502736023943 27.00964934900566 30.6847538661355 24.802860760915838 4 12.250899482645195 17.14066040570796 37.06051237347007 33.54792773817678 5 12.566885636897455 37.776696387600325 34.84624932826719 14.810168647235034 6 31.276349068945986 37.142635729219045 17.845624997038957 13.735390204796014 7 27.61160113771148 32.639223297028316 22.013803465586935 17.73537209967327 8 26.818940308917234 35.74409202475371 19.685681808414195 17.75128585791486 9 26.907688689381416 15.228447524665361 19.997529656808418 37.8663341291448 10-11 25.42642314197478 26.125193990211997 28.295921620171054 20.15246124764217 12-13 25.562433502898124 23.897975510115682 28.591571077787737 21.94801990919846 14-15 22.833982052443037 25.13055598983978 26.270416716581153 25.76504524113603 16-17 22.463090328021302 27.99867819584901 26.909255742369336 22.628975733760353 18-19 22.315346018835022 27.000768520896763 28.98734935416834 21.696536106099877 20-21 23.034911112246657 26.234061439198868 29.042435936960135 21.68859151159434 22-23 22.81245574320203 26.099400197187567 28.955929577610156 22.132214482000247 24-25 22.46139622805603 26.27503147573082 29.049007387173404 22.21456490903975 26-27 22.467138051570338 26.355378239600903 28.98966397032336 22.187819738505404 28-29 22.54511662912588 26.397982678896305 29.016638872043664 22.04026181993416 30-31 22.806592751344077 26.37396645621003 29.050342757689247 21.76909803475665 32-33 22.6310218120953 26.47372254029319 28.93170344430359 21.963552203307923 34-35 22.843937857991435 26.481522561444955 28.883092947863776 21.791446632699834 36-37 22.808090953514785 26.51448187548985 28.813040523803014 21.864386647192354 38-39 22.823390503147493 26.64378655813082 28.642065050778037 21.890757887943646 40-41 22.805789420694126 26.552482382911613 28.706416266691708 21.93531192970255 42-43 22.62722906816574 26.640753238050436 28.61658166114441 22.115436032639412 44-45 22.608098840187278 26.835498685965238 28.473308367434274 22.08309410641321 46-47 22.615316097652762 26.713979392719427 28.237663280852882 22.43304122877493 48-49 22.658583535428537 26.817097017203416 28.233365303068346 22.290954144299697 50-51 22.588501411464073 27.09051593948446 28.02726173939955 22.293720909651917 52-53 22.759066813944862 27.091370688931665 27.790603705237853 22.358958791885623 54-55 22.593356670808934 27.06741065022755 28.003532432864148 22.335700246099364 56-57 22.65757074441687 27.122011011690383 27.753544433316225 22.466873810576523 58-59 22.701524302442884 27.08815356798041 27.919120565849436 22.291201563727267 60-61 22.84688894010449 27.20059265696932 27.846772182078894 22.105746220847298 62-63 22.763399432507363 27.22050784634379 27.84277665265985 22.173316068488997 64-65 22.64935802438218 26.944328721839216 27.90021060198053 22.50610265179807 66-67 22.57971830156525 27.09544875139031 27.80567657694961 22.519156370094827 68-69 22.428970748948355 27.08043184620378 27.87042041681234 22.620176988035524 70-71 22.43160953304022 26.955840787507313 27.874741450260988 22.737808229191483 72-73 22.40736441244717 27.293535443240184 27.56706612118472 22.732034023127927 74-75 22.451401418924075 27.27322876043054 27.478383380242178 22.79698644040321 76-77 22.37812965007887 27.15082592676696 27.77124818480888 22.699796238345296 78-79 22.307540654585576 27.19873215399286 27.711319654743804 22.78240753667776 80-81 22.340697171700594 27.326470645468877 27.477814149026987 22.855018033803546 82-83 22.446796916830646 27.349403747755215 27.525957635189886 22.677841700224256 84-85 22.351008872322332 27.452987692138453 27.3885924866619 22.807410948877315 86-87 22.313250867157276 27.340723892080398 27.49135211890644 22.854673121855885 88-89 22.15386948116298 27.27028424752116 27.569596372852168 23.006249898463686 90-91 22.411725360718098 27.33406425869748 27.467837817162145 22.786372563422276 92-93 22.304161612797373 27.33367756339274 27.401305061789632 22.960855762020255 94-95 22.129306087411575 27.207959849398005 27.47838134338791 23.184352719802508 96-97 22.1076346939822 27.248250457724904 27.48018020289664 23.16393464539626 98-99 22.087056940903793 27.179723492945847 27.382697053921856 23.350522512228505 100 21.129670859307467 27.735601660088093 27.68737083446436 23.447356646140076 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 685.0 1 587.0 2 1439.5 3 2168.0 4 1434.5 5 1014.0 6 1532.0 7 2982.0 8 4659.0 9 5451.5 10 4659.0 11 3601.0 12 3528.5 13 3726.5 14 4046.0 15 4304.5 16 4554.0 17 4827.5 18 5114.5 19 5393.5 20 5750.0 21 6361.5 22 7261.5 23 8569.0 24 10415.0 25 12587.5 26 15227.0 27 18503.0 28 22074.5 29 26163.5 30 31143.5 31 36036.0 32 41654.0 33 47930.5 34 53669.0 35 59109.0 36 65707.0 37 72922.5 38 78634.0 39 83124.5 40 86715.0 41 90179.0 42 93627.0 43 96984.0 44 99857.0 45 103755.0 46 107644.5 47 109973.5 48 112696.0 49 114532.5 50 115050.0 51 113229.5 52 109056.5 53 105527.5 54 101379.0 55 94631.5 56 86978.0 57 79578.5 58 71446.5 59 61955.5 60 51390.5 61 41400.5 62 33207.0 63 26017.5 64 19507.5 65 14612.0 66 11088.5 67 8444.0 68 6465.5 69 4865.5 70 3712.5 71 2837.0 72 2196.0 73 1709.0 74 1288.5 75 988.5 76 715.5 77 483.0 78 344.5 79 239.0 80 161.5 81 112.5 82 84.5 83 58.5 84 37.5 85 30.5 86 16.0 87 11.0 88 10.0 89 5.0 90 3.0 91 2.0 92 2.0 93 0.5 94 0.5 95 1.5 96 1.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23062176507713056 2 0.07025249953046558 3 0.0012520917546373926 4 7.783273069367575E-4 5 3.7224349462192753E-4 6 5.752854007793425E-4 7 0.0 8 8.1216762462966E-4 9 0.0 10-11 3.045628592361225E-4 12-13 0.0013197723900231974 14-15 3.38403176929025E-5 16-17 1.015209530787075E-4 18-19 9.4752889540127E-4 20-21 0.00121825143694449 22-23 1.692015884645125E-5 24-25 1.692015884645125E-5 26-27 1.1844111192515874E-4 28-29 0.0015904949315664176 30-31 0.00175969652003093 32-33 0.0030117882746683224 34-35 0.0038408760581444337 36-37 0.0017258562023380274 38-39 0.0029271874804360663 40-41 0.006175857978954706 42-43 0.011674909604051362 44-45 0.007597151322056611 46-47 0.005989736231643742 48-49 0.005989736231643742 50-51 0.004889925906624411 52-53 0.0034855527223689575 54-55 0.005109887971628277 56-57 0.0035363131989083114 58-59 0.00216578033234576 60-61 0.007647911798595965 62-63 0.010253616260949458 64-65 0.00649734099703728 66-67 0.007732512592828222 68-69 0.01326540453561778 70-71 0.003756275263912178 72-73 0.012148674051751998 74-75 0.007123386874355977 76-77 0.00446692193546313 78-79 0.014805138990644845 80-81 0.006345059567419219 82-83 0.014652857561026782 84-85 0.021742404117689854 86-87 0.014534416449101624 88-89 0.015126622008727417 90-91 0.02737681701355812 92-93 0.02150552189383954 94-95 0.026327767165078144 96-97 0.01680171773452609 98-99 0.01634487344567191 100 0.017360082976458982 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2955055.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.04801415082561 #Duplication Level Percentage of deduplicated Percentage of total 1 78.27367328660412 52.48094354157333 2 14.24240342702836 19.098497330343296 3 3.775662980492864 7.594521148345018 4 1.4588673331455506 3.9125663038768037 5 0.7049673747656615 2.3633331259579227 6 0.3966764166691334 1.595781959887851 7 0.2588971259734967 1.215097671611536 8 0.17354171417122297 0.9308501846008552 9 0.12497362092240595 0.754130979367685 >10 0.5593155562858234 6.353698854290703 >50 0.02181117190121525 0.9735440008443768 >100 0.008499196384671982 1.0492548239896324 >500 2.961648564286257E-4 0.1279999333146511 >1k 2.369318851382163E-4 0.3765731826463729 >5k 5.923297128455407E-5 0.2984944147616683 >10k+ 1.1846594256910814E-4 0.8747125445883108 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11694 0.3957286751008019 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10331 0.34960432208537573 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7516 0.2543438277798552 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3581 0.12118217765828385 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.6920158846451252E-5 1.015209530787075E-4 14-15 0.0 0.0 0.0 3.3840317692902504E-5 1.015209530787075E-4 16-17 0.0 0.0 0.0 3.3840317692902504E-5 1.015209530787075E-4 18-19 0.0 0.0 0.0 3.3840317692902504E-5 1.3536127077161002E-4 20-21 0.0 0.0 0.0 3.3840317692902504E-5 1.3536127077161002E-4 22-23 0.0 0.0 0.0 3.3840317692902504E-5 1.3536127077161002E-4 24-25 0.0 0.0 0.0 1.5228142961806127E-4 1.3536127077161002E-4 26-27 0.0 0.0 0.0 3.5532333577547627E-4 1.3536127077161002E-4 28-29 0.0 0.0 0.0 0.0010659700073264288 1.3536127077161002E-4 30-31 0.0 0.0 0.0 0.003180989863132835 1.3536127077161002E-4 32-33 0.0 0.0 0.0 0.008612360852843686 1.3536127077161002E-4 34-35 0.0 0.0 0.0 0.016717116940293833 1.3536127077161002E-4 36-37 0.0 1.015209530787075E-4 0.0 0.028239745114727138 1.3536127077161002E-4 38-39 0.0 1.015209530787075E-4 0.0 0.04842549461854348 1.3536127077161002E-4 40-41 0.0 1.015209530787075E-4 0.0 0.07461790051285001 1.3536127077161002E-4 42-43 0.0 1.015209530787075E-4 0.0 0.0993044122698224 1.3536127077161002E-4 44-45 0.0 1.015209530787075E-4 0.0 0.12945613533419853 1.3536127077161002E-4 46-47 0.0 1.015209530787075E-4 0.0 0.16461622541712423 1.5228142961806127E-4 48-49 0.0 1.015209530787075E-4 0.0 0.2009945669369944 2.03041906157415E-4 50-51 0.0 1.015209530787075E-4 0.0 0.24148450705655222 2.03041906157415E-4 52-53 0.0 1.015209530787075E-4 0.0 0.28447863068538487 3.045628592361225E-4 54-55 0.0 1.015209530787075E-4 0.0 0.3339531751524083 4.0608381231483E-4 56-57 0.0 1.3536127077161002E-4 0.0 0.3846967315329156 4.0608381231483E-4 58-59 0.0 1.3536127077161002E-4 0.0 0.445304740520904 4.0608381231483E-4 60-61 0.0 1.3536127077161002E-4 0.0 0.5055912664908098 4.0608381231483E-4 62-63 3.3840317692902504E-5 1.3536127077161002E-4 0.0 0.5715629658331233 4.0608381231483E-4 64-65 3.3840317692902504E-5 2.3688222385031751E-4 0.0 0.636164132308874 4.2300397116128125E-4 66-67 3.3840317692902504E-5 2.3688222385031751E-4 0.0 0.7094622604317009 4.399241300077325E-4 68-69 3.3840317692902504E-5 2.3688222385031751E-4 0.0 0.7940292143462643 4.399241300077325E-4 70-71 3.3840317692902504E-5 2.3688222385031751E-4 0.0 0.8823355233658934 4.399241300077325E-4 72-73 3.3840317692902504E-5 2.3688222385031751E-4 0.0 0.9683914512589444 5.414450830864401E-4 74-75 3.3840317692902504E-5 2.3688222385031751E-4 0.0 1.053787492956984 5.414450830864401E-4 76-77 3.3840317692902504E-5 2.5380238269676877E-4 0.0 1.148015857572871 5.414450830864401E-4 78-79 3.3840317692902504E-5 2.7072254154322003E-4 0.0 1.2591813011940556 5.414450830864401E-4 80-81 3.3840317692902504E-5 2.7072254154322003E-4 0.0 1.3730201299129796 5.583652419328913E-4 82-83 3.3840317692902504E-5 2.8764270038967124E-4 0.0 1.4904121919896585 6.09125718472245E-4 84-85 3.3840317692902504E-5 3.045628592361225E-4 0.0 1.6072289686655579 6.09125718472245E-4 86-87 3.3840317692902504E-5 3.045628592361225E-4 0.0 1.7308307290388842 6.260458773186962E-4 88 3.3840317692902504E-5 3.045628592361225E-4 0.0 1.8280201214529002 6.429660361651475E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5515 0.0 47.944515 1 TACCTGG 3645 0.0 44.89998 2 GTACCTG 3775 0.0 44.07368 1 TAACGCA 370 0.0 38.100533 4 ACCTGGG 4270 0.0 37.856644 3 GTATCAA 9245 0.0 37.52256 1 TATAACG 405 0.0 34.83619 2 ATAACGC 470 0.0 30.993841 3 CCTGGGG 3775 0.0 29.6259 4 ATCAACG 11805 0.0 29.058182 3 TCAACGC 11855 0.0 29.014898 4 CAACGCA 11995 0.0 28.67625 5 TATCAAC 12085 0.0 28.369081 2 AACGCAG 12685 0.0 27.265507 6 GTACATG 10425 0.0 25.679932 1 TACATGG 10710 0.0 24.721985 2 CATGGGG 7285 0.0 23.99523 4 ACGCAGA 14460 0.0 23.626118 7 ACATGGG 10670 0.0 23.51735 3 GTATAAC 910 0.0 23.307337 1 >>END_MODULE