FastQCFastQC Report
Mon 6 Jun 2016
ERR1211562_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211562_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3852735
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114810.2979960988752146No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94200.2445016332553368No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60010.15575947995385098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63350.044.5088961
TACCTGG37000.041.9607962
GTACCTG39200.041.016691
GTATCAA102000.035.6869431
ACCTGGG48350.030.7096463
TAACGCA4050.030.165734
GTACATG130550.029.7245461
TATAACG4150.029.4751992
TCAACGC119600.029.0734144
CAACGCA121650.028.541895
ATCAACG122300.028.5076683
TACATGG133950.028.1684632
AACGCAG127250.027.5769086
TATCAAC128450.027.3967462
CATGGGG86550.027.1455384
ACATGGG133750.027.0157363
ACGCAGA144050.024.1327537
CGCAGAG147350.023.3703278
ATGGGGG53350.023.3379045
ATAACGC5500.023.066693
Produced by FastQC (version 0.11.5)