FastQCFastQC Report
Mon 6 Jun 2016
ERR1211562_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211562_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3852735
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128350.333139964207245No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111090.2883406203644943No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74420.19316148138919495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67150.044.287841
GTACCTG43650.042.6495061
TACCTGG43300.042.462692
GTATCAA114400.037.613691
ACCTGGG51850.034.7107623
TCAACGC138750.030.2770144
ATCAACG139650.030.0822773
CAACGCA141100.029.8726625
AACGCAG146300.028.939746
TATCAAC147100.028.6746672
GTACATG135100.027.9084551
CCTGGGG46450.026.9094244
TACATGG138100.026.7637832
ACATGGG135600.026.0598723
CATGGGG84850.025.751924
ACGCAGA167550.025.2687197
CGCAGAG170600.024.7343318
ATGGGGG53100.022.5659795
TAACGCA5000.022.5553594
GCAGAGT192100.021.5502059
Produced by FastQC (version 0.11.5)